FastQCFastQC Report
Wed 30 Apr 2025
1G2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19974557
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC71

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3602281.8034342388669748Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGTGGTGGACTGCTTGCAGTGCTAGATCGGAAGAGCACACGTCTGAACTC3268971.636566958656455Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2562451.2828569865153956Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA2350301.1766468713173464Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1906060.9542439414300903Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1885270.9438357005865011Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA1729720.8659616330915374Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGGCTGAGACTTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG1398140.699960454692437Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATGGGGGACAAGAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1240900.6212403108614625Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT1229760.6156632159601837Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT1180670.5910869512650518Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACTGCTGCCGGTCGTTGGGGCAGATCGGAAGAGCACACGTCTGAACTCCA916840.4590039218391677Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT884680.4429034396107008Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAATCGGCCGTGGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC852690.4268880656527201Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAACTGGGCGTCGGAGGTGCCGTGGAAGGTAGGTAGATCGGAAGAGCACA800380.40069975018720067No Hit
CGACTGCTGCCGGTCGTTGGGGCAGATCGGAAGAGCACACGTCTGAACTC796750.39888243829387554Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACTGCTGCCGGTCGTTGGGGCGAGATCGGAAGAGCACACGTCTGAACT789400.3952027571875561Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGTTAGGCGAACAAGAATAGAGGCCAGATCGGAAGAGCACACGTCTGAA776640.3888146305322316Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGGGGGACAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA718030.3594723026898669Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGTGTAGAATAAGTGGGAGCAGCTAGATCGGAAGAGCACACGTCTGAA706400.35364989571483363Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTGAACTCCAG697540.3492142529118418Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTG656020.3284278094377763Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGG655320.3280773636181268TruSeq Adapter, Index 15 (97% over 43bp)
CGAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC623320.312056983291294Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGACTGCTGCCGGTCGTTGGGGCGGTAGATCGGAAGAGCACACGTCTGAA592870.29681259013654215Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAATCGGCCGTGGATTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT589900.29532569858745805Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGCGACTGCTGCCGGTCGTTGGGGCGGTAGATCGGAAGAGCACACGTCT537540.2691123512776779Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTCTATTTTGTTGGTTTCTGGAACCGAAGTAATAGATCGGAAGAGCACA488040.24433082545960846No Hit
GACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCTG479880.24024562847626607Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGCTGTGACCCTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC458030.2293067125343506Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTGACTGGCGCCGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC451970.22627285300995661Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAGGAATCGGCCGTGGATTAGATCGGAAGAGCACACGTCTGAACTCCA451760.22616771926406176Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGTTAGGCGAACAAGAATAAGATCGGAAGAGCACACGTCTGAACTCCAG451760.22616771926406176Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA446730.22364951573143774Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGT443150.2218572356823733TruSeq Adapter, Index 15 (100% over 37bp)
GGAATCGGCCGTGGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA439420.2199898601005269Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGGACTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATG437050.21880335068257084TruSeq Adapter, Index 15 (100% over 36bp)
ACGGTTAGGCGAACAAGAATAGAGGCCAGATCGGAAGAGCACACGTCTGA414470.20749896981444946Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCT412810.20666791258499498Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGAACGGACCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA399060.19978415541330902Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA395680.19809200274128733Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTAGATCGGAAGA389050.19477278019232167No Hit
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA384270.19237973588100102Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAATCGGCCGTGGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA378430.18945601647135404Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAATCGGCCGTGGATTGGCAGATCGGAAGAGCACACGTCTGAACTCCAG377740.1891105770205567Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACGGACCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT361620.1810403104309147TruSeq Adapter, Index 1 (100% over 35bp)
GTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTC351480.17596385241484955TruSeq Adapter, Index 15 (100% over 38bp)
GCCGGGAAGTTGTTCAAACTTGATCAAGATCGGAAGAGCACACGTCTGAA342130.1712828975381031Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTAGTTGGATTTCGGGATGGCCCGAGATCGGAAGAGCACACGTCTGAAC330140.16528026128439294Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGGTGGACTGCTTGCAGTAGATCGGAAGAGCACACGTCTGAACTCCAG309030.15471181663753544Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGTTAGGCGAACAAGAATAGAGGCAGATCGGAAGAGCACACGTCTGAAC300580.15048143495748115Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGAGCTGTTCTTCCTCGCCGAGACCAGATCGGAAGAGCACACGTCTGAA298540.14946013571164557Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTGCTGCCGGTCGTTGGGGCAGATCGGAAGAGCACACGTCTGAACTCC291470.14592063293318594Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTAGATCGGAAGAG290390.14537994509715535No Hit
GGGGACAAGAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATG290120.1452447731381477TruSeq Adapter, Index 15 (100% over 36bp)
CCGCGACTGCTGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA285150.14275660781863647Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTGGTGGACTGCTTGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC280460.14040862082698505Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAGACCGAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTC269280.13481150045029786Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGACCGAGTGTGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA267460.13390034131920925Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGAACGGACCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC265110.13272384463895745Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAGGAATCGGCCGTGGATTGGCAGATCGGAAGAGCACACGTCTGAACT255200.12776253310649144Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGAAGGAATCGGCCGTGGATAGATCGGAAGAGCACACGTCTGAACTCCAG254000.1271617688442352Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GATAGGAATAGAACGATGTTTCCTTAGACGTAGATCGGAAGAGCACACGT253440.12688141218851562No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACCAGATCGGAAGAGCACACGTCTGA252090.12620555239347736Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTAGTACTGTGAAACTGCGAATGGCTAGATCGGAAGAGCACACGTCTGAA246820.12356719600840207Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGGCCGTGGATTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA235980.11814029217268747Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGTGGACTGCTTGCAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA235700.11800011384482768Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGCTGCCGGTCGTTGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAG235500.11789998646778499Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTGAACTCCAGT223550.11191737568948337Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGCGACTGCTGCCGGTCGTTGGGGCGGTGTTCTAGATCGGAAGAGCACA219380.10982971987814297No Hit
GACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC215210.10774206406680258Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAGGTGTAGAATAAGTGGGAGCAGCTCGCAGATCGGAAGAGCACACGTCT212240.1062551725177184Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTAACTGAGTGTGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC206180.10322131299332446Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)