FastQCFastQC Report
Wed 30 Apr 2025
1G12_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G12_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10878910
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC70

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG3355763.0846472670515706Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA2971602.7315236544837673Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG2695082.477343778007172Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT1982321.8221678458595576Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC1936681.7802151134626538Illumina RNA PCR Primer (100% over 30bp)
CAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT1864851.7141882780535918Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC1753681.6119997315907568Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG1424131.3090741627607914Illumina DpnII expression Sequencing Primer (96% over 29bp)
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA1288071.1840064859439043Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG1103441.0142927922006892Illumina Small RNA Adapter 1 (100% over 26bp)
ATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCT1070590.9840967523400782Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG789550.7257620478522204Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG719860.6617023212803489Illumina Small RNA Adapter 1 (100% over 26bp)
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAG658380.6051893066492875No Hit
GCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGT553940.5091870417164954Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC549290.5049127164394227Illumina DpnII expression Sequencing Primer (100% over 21bp)
CAGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAG547580.5033408677891443Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCTCTATTCTTGTTCGCCTAACCGGATCGTCGGACTGTAGAACTCTGA421270.3872354859080551Illumina DpnII expression Sequencing Primer (100% over 24bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA380020.34931808425660293Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCCTCTATTCTTGTTCGCCTAACCGTGATCGTCGGACTGTAGAACTCTG364820.3353460962541284Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT359640.330584589816443Illumina RNA PCR Primer (100% over 44bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC355860.32710997701056443No Hit
AGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA350810.32246796783868975Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTA350460.32214624443073797No Hit
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCGATCGTCGGACTGT321640.29565461981025676No Hit
TCAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA313450.28812629206418655Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCAATCCACGGCCGATTCCTTCCCGGGATCGTCGGACTGTAGAACTCT299970.2757353448093605Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCTCTTGTCCCCCATGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT285010.26198396714376715Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCGCGGTCCGTTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA283320.26043050268822887Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA269590.2478097529991516Illumina DpnII expression Sequencing Primer (100% over 25bp)
GACCGCGGTCCGTTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT267670.24604487030410216Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT259840.23884745806335375No Hit
CCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGT259220.23827754802641074Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCTCTATTCTTGTTCGCCTAACCGGATCGTCGGACTGTAGAACTCTGAA256900.23614498143655935Illumina DpnII expression Sequencing Primer (100% over 25bp)
TGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG255940.23526254008903466Illumina DpnII expression Sequencing Primer (96% over 29bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA253290.23282663428597167Illumina RNA PCR Primer (100% over 45bp)
GCCTCGACCACGGCCAGTCCGATCGTCGGACTGTAGAACTCTGAACGTGT246710.2267782342164794Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC245920.22605205852424554Illumina RNA PCR Primer (100% over 30bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGAC228930.21043468509253224No Hit
CAGTTAAGAGCACACTCGGTCTCGATCGTCGGACTGTAGAACTCTGAACG222980.20496538715735307Illumina Small RNA Adapter 1 (100% over 26bp)
GGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT219530.20179411356468618Illumina RNA PCR Primer (100% over 31bp)
TCCAACCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGTA208260.1914346198286409Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAA204560.1880335438017228Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTA196180.1803305662056217No Hit
GACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG180610.16601847060045538Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGCACGAGACAGAGTGTCGGCGCCAGTCAGGATCGTCGGACTGTAGAACT179380.16488784262393935No Hit
AGAACACCGCCCCAACGACCGGCAGCAGTCGCGGGATCGTCGGACTGTAG176650.16237840004191595No Hit
GACAGGGTCGAGTTCGACTATCTTGGTCGGGATCGTCGGACTGTAGAACT174520.16042048330209552No Hit
AACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC169330.15564978476704008Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAG168800.15516260360642747Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCTCGACCACGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG168190.15460188566685448Illumina RNA PCR Primer (100% over 33bp)
ACCTACCTTCCACGGCACCTCCGACGCCCAGTTCGATCGTCGGACTGTAG164680.15137545948996728No Hit
AACATTCTTGGCAAATGCTTTCGCAGTAGTTCGTCTTTCGATCGTCGGAC163790.15055736282403293No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC162340.14922450870537582No Hit
GGCCTCTATTCTTGTTCGCCTAACCGTAAACGATCGTCGGACTGTAGAAC157850.1450972569862238No Hit
AATCCACGGCCGATTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT155330.14278084844897143Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT153500.14109869463025249Illumina RNA PCR Primer (100% over 50bp)
TTCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCT149280.1372196295400918Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACG148770.1367508325742193Illumina Small RNA Adapter 1 (100% over 26bp)
GCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGAACG147550.13562939669507332Illumina Small RNA Adapter 1 (100% over 26bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCT144130.13248569939451654Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG140220.12889158932282738Illumina DpnII expression Sequencing Primer (96% over 29bp)
ATCCAACCGCTTCAAGTCTGATCGTCGGACTGTAGAACTCTGAACGTGTA139410.1281470294358534Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT138340.12716347501725817Illumina RNA PCR Primer (100% over 31bp)
ATCCAACCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGT136090.12509525310899713Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGATCGTCGGACTGTAGAACTCT134800.1239094725482608Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCCAGTGATCGTCGGA134720.12383593576930042No Hit
GGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC128060.11771399892084777Illumina RNA PCR Primer (100% over 32bp)
GCTCTTGTCCCCCATGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC127690.11737389131815595Illumina RNA PCR Primer (100% over 30bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT121490.11167479094872557No Hit
GCCTCGACCACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG117050.10759349971642379Illumina RNA PCR Primer (100% over 34bp)
ACCGCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGA115550.10621468511091645Illumina DpnII expression Sequencing Primer (100% over 24bp)
GGCCAATCCACGGCCGATTCCTTCCGATCGTCGGACTGTAGAACTCTGAA114530.10527709117917143Illumina DpnII expression Sequencing Primer (100% over 25bp)
AACATTCTTGGCAAATGCTTTCGCAGTAGTTCGTCTTTGATCGTCGGACT111570.10255623035763693No Hit
ACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGACT110740.10179328627592286No Hit
TGATCAAGTTTGAACAACTTCCCGGCGATCGTCGGACTGTAGAACTCTGA109930.10104872638894888Illumina DpnII expression Sequencing Primer (100% over 24bp)
TCCAACCGCTTCAAGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA109540.10069023459151698Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACGT109080.10026739811249473Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCTCGACCACGGCCAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA109050.10023982182038459Illumina DpnII expression Sequencing Primer (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)