FastQCFastQC Report
Wed 30 Apr 2025
1G12_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G12_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10878910
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA3096532.8463605269277896Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2703392.484982410921682Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA2542972.337522784911356Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1834681.686455720288154Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1798931.6535939721902286Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1701031.5636033389374489Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT1665171.5306404777684528Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA1338171.2300588937678498Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT1198111.1013143780029433Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTGGTGGACTGCTTGCAGTGCTAGATCGGAAGAGCACACGTCTGAACTC1042070.9578808906406984Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTG1019960.9375571633555201Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGCTGTGACCCTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC738270.6786249725386091Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC682250.6271308430715945Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCAGATCGGAAGAGCACA655230.602293795977722No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCT534090.4909407284369482Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGGGCTGAGACTTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG519250.4772996559397954Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC500980.4605056940447159Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGTTAGGCGAACAAGAATAGAGGCCAGATCGGAAGAGCACACGTCTGAA406910.3740356340846647Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC356460.3276615028527674Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGTTAGGCGAACAAGAATAGAGGCCAGATCGGAAGAGCACACGTCTGA354770.3261080383972291Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCAGATCGGAAGAGCAC351570.3231665672388135No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG349360.3211351137200326No Hit
CGAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA328360.3018317092429297Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCA316250.2907000793278003No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTG289070.26571595867600706Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGTGCTCTTAACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA288770.2654401957549056Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGGGGGACAAGAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC269810.24801197914129264Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGAACGGACCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA269070.247331763935909Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCG255090.2344812118125805No Hit
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC253180.23272552121490114Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGAACGGACCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC251700.23136509080413387Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGGGCTGAGACTTGAAGCAAGATCGGAAGAGCACACGTCTGAACTCCA247090.22712753391654128Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGTTAGGCGAACAAGAATAGAGGCAGATCGGAAGAGCACACGTCTGAAC245460.2256292220452233Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCC239700.22033457396007505Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAG230380.21176753921118935Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTTTCCGAGGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT227610.20922132823968578Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGA223910.20582025221276765No Hit
GAGACCGAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTC207770.19098420705750851Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTGGATCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC206530.18984438698362244TruSeq Adapter, Index 5 (97% over 35bp)
CCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCAC193730.1780785023499597No Hit
GAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT191370.17590916737062812Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCGGGCTGAGACTTGAAGCGGTTGAGATCGGAAGAGCACACGTCTGAAC191020.17558744396267642Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCGACTGCTGCCGGTCGTTGGGGCGGTGTTCTAGATCGGAAGAGCACA174090.1600252231151834No Hit
CTGACTGGCGCCGACACTCTGTCTCGTGCAAGATCGGAAGAGCACACGTC172600.1586556006070461No Hit
CTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC171220.15738709116997934Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTC170540.15676202854881602No Hit
CGACCAAGATAGTCGAACTCGACCCTGTTAGATCGGAAGAGCACACGTCT161950.1488660169079439Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAACTGGGCGTCGGAGGTGCCGTGGAAGGTAGGTAGATCGGAAGAGCACA161480.14843398833155158No Hit
GAAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTAGATCGGAAGA161110.1480938807288598No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT159910.1469908290444539No Hit
CGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGA156910.14423319983343919TruSeq Adapter, Index 10 (97% over 36bp)
GAGTGTGCTCTTAACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC156440.1438011712570469Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTTTACGGTTAGGCGAACAAGAATAGAGGCCAGATCGGAAGAGCACACGT153350.14096081316970174No Hit
GAATCGGCCGTGGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC143330.13175033160491262Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGGGCTGAGACTTGAAGCGGTAGATCGGAAGAGCACACGTCTGAACTC141290.12987514374142262Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTTCCGAGGTCGCGGTCGGACCGGGAAAGATCGGAAGAGCACACGTCTG141210.12980160696246223Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCTGCCGGTCGTTGGGGCAGATCGGAAGAGCACACGTCTGAACTC140470.1291213917570786Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTG136100.12510444520636718Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGGCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAG135170.12424958015095262No Hit
GACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCTG130720.12015909682128081Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGACTGGCCGTGGTCGAGGTAGATCGGAAGAGCACACGTCTGAACTCCA129910.11941453693430684Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTGAACTCCAGT129870.11937776854482664Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGTGTGCTCTTAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC128280.11791622506298885TruSeq Adapter, Index 5 (97% over 35bp)
GAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTGAACTCCAG125830.11566416120732682Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTGGATCCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCG120040.11034193683006845TruSeq Adapter, Index 10 (97% over 36bp)
ATGGGGGACAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT119540.109882331961566Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG118510.10893554593245096No Hit
AAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTAGATCGGAAGAG110420.1014991391600813No Hit
GGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTGAAC109110.10029497440460487Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGACTGCTGCCGGTCGTTGGGGCGGTAGATCGGAAGAGCACACGTCTGAA108930.10012951665194399Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)