FastQCFastQC Report
Wed 30 Apr 2025
1G11_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G11_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10305459
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC70

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG4109273.9874691656140695Illumina RNA PCR Primer (100% over 37bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA3370873.2709557138600034Illumina DpnII expression Sequencing Primer (100% over 25bp)
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG3258723.162129896397628Illumina RNA PCR Primer (100% over 36bp)
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG3213603.11834727594375Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC2473062.399757254868512Illumina DpnII expression Sequencing Primer (100% over 26bp)
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA2161212.097150646079908Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC1481281.4373741140496508No Hit
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG1326121.286813134669693Illumina Small RNA Adapter 1 (100% over 26bp)
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG1127681.0942549963082673Illumina DpnII expression Sequencing Primer (96% over 29bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA840970.8160432252459594Illumina RNA PCR Primer (100% over 45bp)
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA798590.7749193898107789Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC721430.7000464511090676No Hit
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG668020.6482195504343863Illumina DpnII expression Sequencing Primer (100% over 23bp)
ATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCT551520.5351726691649542Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT503250.48833341629907023Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGT483830.46948903488917865Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGAAC472900.45888300559926537Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG470250.4563115529351968Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAG447590.43432320675867037No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT437720.4247457585343845Illumina RNA PCR Primer (100% over 50bp)
CAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA426670.4140232861049663Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAA416700.404348801931093Illumina DpnII expression Sequencing Primer (100% over 25bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC409690.3975465818650096Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTA400970.38908504706098No Hit
GGTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTAGAACTCTGA397220.38544619895144894Illumina DpnII expression Sequencing Primer (100% over 24bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT376030.3648842812338587Illumina DpnII expression Sequencing Primer (100% over 22bp)
TTCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCT364730.3539192189304717Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT332090.3222466849851132No Hit
GGCCAATCCACGGCCGATTCCTTCCCGGGATCGTCGGACTGTAGAACTCT331950.32211083465569074Illumina DpnII expression Sequencing Primer (100% over 22bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGAC320750.31124280830189127No Hit
CGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACGT310530.30132573425404924Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACG303120.29413537038961585Illumina Small RNA Adapter 1 (100% over 26bp)
CGCGACCTCGGAAAGGGAGAGAGAACCCCCCCTCGCCTCGACCACGGCCA295220.2864695303722037No Hit
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCGATCGTCGGACTGT288950.28038537633306776No Hit
GCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGT268780.26081322530126994Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGT268230.2602795275785387Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAA261710.253952783665434Illumina DpnII expression Sequencing Primer (100% over 25bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT257120.24949883357936797No Hit
TGACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACT244370.23712675000696232No Hit
CGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC240980.23383723131594625Illumina RNA PCR Primer (100% over 30bp)
GACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG240950.23380812053107Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGCTAGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC226740.2200193120946869Illumina RNA PCR Primer (100% over 32bp)
AGCCGGGCTGTAACAGTCCGGAGCCGAGACTCCAGGATCGTCGGACTGTA222630.21603113456664086No Hit
GGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTA210540.20429948826151267No Hit
GGCCAGTCCGGGCCGGATCCGATCGTCGGACTGTAGAACTCTGAACGTGT206020.19991346334015786Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATGATCGTCGGACTG201140.19517810900028812No Hit
AGATTAAGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGG195940.19013223962173834No Hit
AGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC192570.18686212812063976Illumina RNA PCR Primer (100% over 30bp)
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC191190.18552303201633233No Hit
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCCAGTAACGTAGATC187840.18227232770515123No Hit
AGCTGCTCCCACTTATTCTACACCTCGATCGTCGGACTGTAGAACTCTGA187790.18222380973035748Illumina DpnII expression Sequencing Primer (100% over 24bp)
TGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG185420.17992405772513384Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTC184720.17924480607802137Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG179090.1737816821162454Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT172470.16735790225355318Illumina RNA PCR Primer (100% over 31bp)
AGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG168330.16334061394063087Illumina Small RNA Adapter 1 (100% over 26bp)
CAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT167140.1621858861405397Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGGAAACGTTCGAGTTGGCTATCGGTAGTGGATCGTCGGACTGTAGAACT159700.15496641149123005No Hit
GCGAGCTGCTCCCACTTATTCTACACCTCGATCGTCGGACTGTAGAACTC156430.15179333593971894Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGTCTCGGCGAGGAAGAACAGCTCGGCGATCGTCGGACTGTAGAACTCTG152280.1477663440318379Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCCAATCCACGGCCGATTCCTTCCGATCGTCGGACTGTAGAACTCTGAA146080.14175011515741318Illumina DpnII expression Sequencing Primer (100% over 25bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGGATCGTCGGACTGTAGAACT144120.13984821054549826No Hit
TCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGA143580.1393242164177258Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG139010.13488967352157727No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT131230.12734027664367012No Hit
GCCTCGACCACGGCCAGTCCGATCGTCGGACTGTAGAACTCTGAACGTGT129230.1253995576519202Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG124970.12126582619949292Illumina RNA PCR Primer (100% over 37bp)
GGCCAGTCCGGGCCGGATCCAAGATCGTCGGACTGTAGAACTCTGAACGT120590.11701565160756061Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA119870.11631699277053063Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTA119320.11578329504779941Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTCT117610.11412398030985327Illumina DpnII expression Sequencing Primer (100% over 22bp)
TTTTCCTCCAATCCTCACGGCATAAACAAGATCGTCGGACTGTAGAACTC114020.11064038971966217Illumina DpnII expression Sequencing Primer (100% over 21bp)
AACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC113200.1098446949330447Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGTTAAGAGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGGATCGTCG108100.10489586150408245No Hit
ATCCAACCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGT106970.10379935527374375Illumina DpnII expression Sequencing Primer (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)