FastQCFastQC Report
Wed 30 Apr 2025
1G11_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G11_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10305459
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACAT3845593.7316047737417617TruSeq Adapter, Index 2 (97% over 37bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC3182323.087994430912781Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACA3067122.9762090169879865TruSeq Adapter, Index 2 (97% over 37bp)
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA2971662.883578499511764Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT2354952.285148094810721Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC2042741.982192156603602Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG1466131.4226731676871451No Hit
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC1260241.222885851081451Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA1056781.0254565080507332Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT740030.7180951377323417Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT709680.6886447270325369No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA634320.6155184354234003Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTG526400.5107972386285754Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC466830.4529929234593044Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGGGCTGAGACTTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG454360.4408925405457438Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA448060.43477927572173153Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCAGATCGGAAGAGCACA445410.4322078230576629No Hit
CGACTGCTGCCGGTCGTTGGGGCGAGATCGGAAGAGCACACGTCTGAACT442910.42978192431797557Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCT399480.3876392114121263Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAAC397070.3853006450270677Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCAC396160.3844176178858215No Hit
GAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA389670.378119984757593Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGAGCTGTTCTTCCTCGCCGAGACCAGATCGGAAGAGCACACGTCTGAA387420.3759366758918744Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGACTGGCCGTGGTCGAGGCGAGGGGGGGTTCTCTCTCCCTTTCCGAGG380870.36958082119389346No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG362110.35137687705127935Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTCCGAGGTCGCGGTCGGACCGGGAAAGATCGGAAGAGCACACGTCTG347050.33676326304340254Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG327150.31745310907549096No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTG321080.31156302693553Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGA309000.2998410842253606No Hit
CTCGGGCTGAGACTTGAAGCGGTAGATCGGAAGAGCACACGTCTGAACTC288280.2797352354708315Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCC287330.2788133939497503Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCA283180.27478640204186927No Hit
GGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCG251920.24445296420081822No Hit
GTTGGATCCGGCCCGGACTGGCAGATCGGAAGAGCACACGTCTGAACTCC251390.2439386736680045Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCC248670.24129929583922463Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGGGCTGAGACTTGAAGCGGTTGAGATCGGAAGAGCACACGTCTGAAC246080.23878606474490852Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGACCAAGATAGTCGAACTCGACCCTGTCAAGATCGGAAGAGCACACGTC235810.2288204727222727No Hit
CTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC226620.21990286895518193Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTTTCCGAGGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC222590.21599232018680586Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGAGTCTCGGCTCCGGACTGTTACAGCCCGGCTAGATCGGAAGAGCAC217320.21087852564354487No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCAGATCGGAAGAGCAC211090.2048331859842439No Hit
GGGGGACTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTA209660.2034455719051427TruSeq Adapter, Index 2 (97% over 36bp)
ATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGAGC194090.18833707455436968No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAATCTAGATCGGAA192580.18687183171559849No Hit
GGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG191170.18550362482641483Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TACGTTACTGGCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGAT190060.1844265257859936No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT185110.1796232462814126No Hit
GAGGTGTAGAATAAGTGGGAGCAGCTAGATCGGAAGAGCACACGTCTGAA178510.1732188736086379Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC178360.17307331968425668Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGGGCTGAGACTTGAAGCAAGATCGGAAGAGCACACGTCTGAACTCCA177620.1723552536573092Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT175000.1698129117781168Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGCTGTGACCCTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC166880.1619335926716122Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTTGGATCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT162180.15737290304099993TruSeq Adapter, Index 12 (100% over 35bp)
CGTGGTGGACTGCTTGCAGTGCTAGATCGGAAGAGCACACGTCTGAACTC160560.1558009206576825Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACTACCGATAGCCAACTCGAACGTTTCCGAGATCGGAAGAGCACACGTC154370.14979439537821654No Hit
AGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC153310.1487658143125891Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCTGTGAATAGGCGGTTGCTCACAGCCCGACCAAGATAGTCGAACTCGAC150890.1464175443325717No Hit
GAGGTGTAGAATAAGTGGGAGCAGCTCGCAGATCGGAAGAGCACACGTCT148250.14385579526346182Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAGCTGTTCTTCCTCGCCGAGACCAGATCGGAAGAGCACACGTCTGA146170.14183744751204191Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTC140550.13638402714522468No Hit
GGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTGAAC140490.1363258055754722Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGGATCCGGCCCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA132570.12864055836814256Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACATCTGGGGGG130580.1267095429713514TruSeq Adapter, Index 2 (97% over 37bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC127900.12410897952240653No Hit
GGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAG120500.11692831925293187Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC119380.11584151661755192No Hit
CGACCAAGATAGTCGAACTCGACCCTGTAGATCGGAAGAGCACACGTCTG113890.11051424298519842Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCC113640.11027165311122969Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACAT112760.10941773675485972TruSeq Adapter, Index 2 (97% over 37bp)
AGTGTGCTCTTAACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA110750.10746731416815107Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTTGGATCCGGCCCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGT110490.10721502069922359Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGACCAAGATAGTCGAACTCGACCCTGTTAGATCGGAAGAGCACACGTCT109060.10582740662012241Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGTTTATGCCGTGAGGATTGGAGGAAAAAGATCGGAAGAGCACACGTCT108550.10533252327722617Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTCTTAACTAGATCGGA108050.1048473435292887No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)