FastQCFastQC Report
Wed 30 Apr 2025
1F9_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F9_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13133636
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC70

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG175681913.376486145953795Illumina RNA PCR Primer (100% over 37bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA133626710.174387351682352Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC3605822.7454849517681166No Hit
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG3124802.3792345090118228Illumina RNA PCR Primer (100% over 36bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC2141231.6303406002724607No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1910691.4548065745083845Illumina RNA PCR Primer (100% over 50bp)
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG1352261.02961586570543Illumina DpnII expression Sequencing Primer (96% over 29bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1194740.9096795434257504Illumina RNA PCR Primer (100% over 45bp)
AAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG999180.7607794216316031Illumina RNA PCR Primer (100% over 37bp)
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG901920.6867252906963465Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC711980.5421042581049147Illumina RNA PCR Primer (100% over 30bp)
ACTCGTTCTCAAAGTAGTTCAGCAGAAGATCGTCGGACTGTAGAACTCTG695940.529891341590402Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA673940.5131404585904468Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT630200.47983665757144484Illumina DpnII expression Sequencing Primer (100% over 22bp)
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA592570.4511850336037941Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT500000.38070188636261887Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCTTCCTTAGGGAGTTACACAAGATCGTCGGACTGTAGAACTCTGAACGT478280.3641641964190267Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT463190.35267461348860285No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC458530.34912647190770324No Hit
TAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG451290.34361390859317253Illumina RNA PCR Primer (100% over 37bp)
GAGCTAGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC415530.316386109680518Illumina RNA PCR Primer (100% over 32bp)
CAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT390630.2974271557396596Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC365200.2780646577992568Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT359500.273724656294723No Hit
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG343940.26187721359111826Illumina DpnII expression Sequencing Primer (100% over 23bp)
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG336720.256379878352042No Hit
CCACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGT311280.237009766373912Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA301470.22954039536347742No Hit
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC282460.21506610964397063Illumina DpnII expression Sequencing Primer (100% over 26bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT277720.211457055761253No Hit
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG259090.19727210347538185Illumina Small RNA Adapter 1 (100% over 26bp)
ACAAAATGGACAGGCTTCTTGTAGACAAGATCGTCGGACTGTAGAACTCT258720.1969903840794735Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGAAC231980.17663044719680063Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTA224520.17095037505227037No Hit
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGAC219900.16743268962227975No Hit
GCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGT209480.1594988623104828Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGTGT202360.1540776674486791Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCT188040.14317436542325368Illumina DpnII expression Sequencing Primer (100% over 22bp)
ATCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA185260.14105766293507754Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGCCGGGCTGTAACAGTCCGGAGCCGAGACTCCAGGATCGTCGGACTGTA182780.13916938157871894No Hit
AAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG182700.13910846927690093Illumina RNA PCR Primer (100% over 36bp)
ACTCATCCTAAGTAGACTTTTCACATTGAAGATCGTCGGACTGTAGAACT179280.13650446837418062No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG177970.13550702943191054No Hit
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAG177290.1349892748664574No Hit
CCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAACG176780.1346009589423675Illumina Small RNA Adapter 1 (100% over 26bp)
AAACTAACCAGCCTAGACTTGAGATCGTCGGACTGTAGAACTCTGAACGT172600.13141829117237602Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGGTCCGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT166830.1270249914037514Illumina RNA PCR Primer (100% over 35bp)
CTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA160940.12254032318239977Illumina DpnII expression Sequencing Primer (100% over 25bp)
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC156980.11952516424240782No Hit
ACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG156400.11908355005422717Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC156250.1189693394883184No Hit
TGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG147020.11194158266606445Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGAACG146830.11179691594924665Illumina Small RNA Adapter 1 (100% over 26bp)
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACCCATCCGATCGTCGGA134880.10269814086518007No Hit
AACATTCTTGGCAAATGCTTTCGCAGTAGTTCGTCTTTCGATCGTCGGAC131850.10039108743382259No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)