FastQCFastQC Report
Wed 30 Apr 2025
1F9_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F9_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13133636
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC73

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGAT165794612.62366339374717TruSeq Adapter, Index 22 (97% over 41bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC12570119.570929177571237Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT3487732.6555707802469932No Hit
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGA2963972.256777940244423TruSeq Adapter, Index 22 (97% over 39bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG2107131.60437673162253No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTGGGGGGGGGGG1458011.1101343146711238TruSeq Adapter, Index 22 (97% over 39bp)
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA1246720.9492573115320084Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTGGGGGG1051900.8009206285296775TruSeq Adapter, Index 22 (97% over 43bp)
CGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGAT934710.7116917204040069TruSeq Adapter, Index 22 (97% over 41bp)
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA851210.6481145053814497Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC665160.5064553334659191Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCTGCTGAACTACTTTGAGAACGAGTAGATCGGAAGAGCACACGTCTGA659170.501894524867295Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA607330.46242335328921863Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG599080.4561417721642354Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT555410.42289126940932426Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC465930.35476085982587Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC446000.339586082635456No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT445100.3389008192400033No Hit
TTGTGTAACTCCCTAAGGAAGGAGATCGGAAGAGCACACGTCTGAACTCC440800.33562678301728477Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGACCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGAT413230.31463488100325TruSeq Adapter, Index 22 (97% over 41bp)
GGGGGACTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGT389380.296475401023753TruSeq Adapter, Index 22 (100% over 36bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG354070.2695902338088249No Hit
AGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC351820.2678770753201931Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT347510.26459542505974737Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA323480.24629889240115987Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC305190.2323728173980153No Hit
ACCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC292890.22300755099349487Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG289850.22069288352441016No Hit
GGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCG272080.2071627384830827No Hit
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT263230.20042431509446432Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC246000.18730532809040848Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGTCTACAAGAAGCCTGTCCATTTTGTAGATCGGAAGAGCACACGTCTG244450.18612515224268436Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCAGATCGGAAGAGCAC219280.16696061928319011No Hit
CGACTGCTGCCGGTCGTTGGGGCGAGATCGGAAGAGCACACGTCTGAACT211550.16107496812002403Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGA210430.1602221958945718No Hit
CTCGGGCTGAGACTTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG196970.14997370111369007Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG192570.14662352451369903Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAAAATCTTTGCTCTGAAGTGGATAGATCGGAAGAGCACACGTCTGAAC181220.13798159169326757Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTG178370.13581159094100065Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGGAGTCTCGGCTCCGGACTGTTACAGCCCGGCTAGATCGGAAGAGCAC174460.132834502189645No Hit
CCGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGA173200.13187513343601115TruSeq Adapter, Index 22 (97% over 39bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCAGATCGGAAGAGCACA172690.13148681751192132No Hit
CGGACCTCAGATCGGAGAGCACACGTCTGAACTCCAGTCACCGTACGATC172110.13104520332374067TruSeq Adapter, Index 22 (97% over 35bp)
TTCAATGTGAAAAGTCTACTTAGGATGAGTAGATCGGAAGAGCACACGTC170370.12972036075919874No Hit
TCAAGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC163170.12423825359557704Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAAAATCTTTGCTCTGAAGTGGAGATCGGAAGAGCACACGTCTGAACTC162620.12381948152057816Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACG158930.12100990159922202TruSeq Adapter, Index 22 (100% over 39bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCGG156170.11890842718650038No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT150950.11493389949287464No Hit
CAGCTGTGACCCTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC148130.11278674085378945Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAAAATCTTTGCTCTGAAGTGGAGAGATCGGAAGAGCACACGTCTGAAC147670.11243649511833585Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGGGCTGAGACTTGAAGCAAGATCGGAAGAGCACACGTCTGAACTCCA144020.10965737134788874Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGACTGCTGCCGGTCGTTGGGGCAGATCGGAAGAGCACACGTCTGAACTC140140.10670312470971481Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)