FastQCFastQC Report
Wed 30 Apr 2025
1F5_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F5_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17824415
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA229586612.88045638524462Illumina DpnII expression Sequencing Primer (100% over 25bp)
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG10025745.624723167632711Illumina RNA PCR Primer (100% over 37bp)
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG7997974.4870869534848685Illumina RNA PCR Primer (100% over 36bp)
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA7965254.46873010979603Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC4631352.5983180934689862No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC4575212.5668219686312286No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT2021671.1342139419442379No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1686630.9462470437318701Illumina RNA PCR Primer (100% over 50bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1370310.7687825939869555Illumina RNA PCR Primer (100% over 45bp)
ACTCGTTCTCAAAGTAGTTCAGCAGAAGATCGTCGGACTGTAGAACTCTG1261700.7078493179159036Illumina DpnII expression Sequencing Primer (100% over 23bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT1177360.6605321969893542Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1064960.597472623926227No Hit
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC984030.5520686092643153No Hit
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG947130.5313666675736622No Hit
AAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG881420.4945015025738572Illumina RNA PCR Primer (100% over 37bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC878800.49303160861099793No Hit
AAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG730340.4097413575705009Illumina RNA PCR Primer (100% over 36bp)
CCACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGT717140.40233578493319416Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA686420.38510099770455297No Hit
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC487160.273310512575027Illumina DpnII expression Sequencing Primer (100% over 26bp)
AATGAGGTCAAAGTAGCGGATGAGGGGTGGATCGTCGGACTGTAGAACTC476780.2674870395465994Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCTTCCTTAGGGAGTTACACAAGATCGTCGGACTGTAGAACTCTGAACGT474670.26630326998109055Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCATCCTAAGTAGACTTTTCACATTGAAGATCGTCGGACTGTAGAACT468100.26261731450934017No Hit
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC448550.2516492126109048No Hit
ATCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA438660.2461006434152257Illumina DpnII expression Sequencing Primer (100% over 25bp)
TTCCACTTCAGAGTAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA426850.23947490001775654Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCTTTTGCTTCTGCTACAATCTTCAACTTAGATCGTCGGACTGTAGAACT410320.23020110337422015No Hit
ACAGACCGAAAAACACAGGATGAATCTCAGTGGATCGTATCGATCGTCGG408600.22923613481844987No Hit
GAGGTCCGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT392140.22000161015102038Illumina RNA PCR Primer (100% over 35bp)
TAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG379240.21276434598274332Illumina RNA PCR Primer (100% over 37bp)
ACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGTGT378670.21244455989158692Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG371110.2082031864720385Illumina Small RNA Adapter 1 (100% over 26bp)
AAACTAACCAGCCTAGACTTGAGATCGTCGGACTGTAGAACTCTGAACGT357890.20078639327012976Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC310560.17423292713954427No Hit
TAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG308510.17308281926784133Illumina RNA PCR Primer (100% over 36bp)
GAGCTAGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC288920.16209227624020198Illumina RNA PCR Primer (100% over 32bp)
TTTCCTCCAATCCTCACGGCATAAACAAGATCGTCGGACTGTAGAACTCT282070.15824923286402387Illumina DpnII expression Sequencing Primer (100% over 22bp)
AAGCTCAGAGCATGTTTCTTATCACTGGACATCAGCGTATATTAAAGACA278720.15636978829319223No Hit
TTTTTTTTTCCTTCACGAGTAAACATTCTAGATCGTCGGACTGTAGAACT266040.14925595033553696No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG259130.1453792452655529No Hit
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG254200.14261337609116484Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAACG242860.13625131596184223Illumina Small RNA Adapter 1 (100% over 26bp)
TTTTCCTCCAATCCTCACGGCATAAACAAGATCGTCGGACTGTAGAACTC239570.13440553308481654Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAACTCTGAAC236910.1329131979927532Illumina DpnII expression Sequencing Primer (100% over 26bp)
TTTCTGAATCACTGTCCATCCTGATCGTCGGACTGTAGAACTCTGAACGT232650.13052321773253148Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACAGATCG231430.1298387632918107No Hit
CCTTGAGAGATCGGAGCTAGTCCCCGTTGTAGATCGTCGGACTGTAGAAC225950.1267643285908682No Hit
TCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC221630.12434068663684053Illumina DpnII expression Sequencing Primer (100% over 26bp)
CTTGAGAGATCGGAGCTAGTCCCCGTTGTAGATCGTCGGACTGTAGAACT216480.1214513912518307No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT206780.11600941741987045No Hit
AATGGAGGTCCCACCGAGAGATCGTCGGACTGTAGAACTCTGAACGTGTA203870.11437682527028235Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAGATCGTCG198090.11113408210031016No Hit
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT196300.11012984156843295No Hit
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG181860.10202859392580345Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCCATTACCAGTTTAGAATCCATGATCAGATCGTCGGACTGTAGAACTC180550.10129364694437376Illumina DpnII expression Sequencing Primer (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)