FastQCFastQC Report
Wed 30 Apr 2025
1F5_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F5_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17824415
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC70

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC216430912.142384476573284Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTAT9239295.183502516071354TruSeq Adapter, Index 3 (97% over 37bp)
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC7502964.209372369303565Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTA7433874.170610928886025TruSeq Adapter, Index 3 (97% over 37bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG4592542.5765445878588444No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT4497172.523039325554303No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG2001031.1226343192749944No Hit
TTCTGCTGAACTACTTTGAGAACGAGTAGATCGGAAGAGCACACGTCTGA1200020.6732450966833975Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTGGGGGG1136170.6374234441915766TruSeq Adapter, Index 3 (97% over 37bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG1128000.6328398435516678Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1035130.580737151822374No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT954130.5352938651843553No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT861050.4830733575267407No Hit
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC814370.45688455974571957No Hit
CGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTAT795750.44643821410127627TruSeq Adapter, Index 3 (97% over 37bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTGGGGGGGGGGG786920.4414843348295021TruSeq Adapter, Index 3 (96% over 33bp)
ACCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC675330.3788791946327551Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG668310.37494077645746016No Hit
CCGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTA663520.37225345123528597TruSeq Adapter, Index 3 (97% over 37bp)
CACCCCTCATCCGCTACTTTGACCTCATTAGATCGGAAGAGCACACGTCT466710.26183748526950257Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT456820.2562889160738234Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCAATGTGAAAAGTCTACTTAGGATGAGTAGATCGGAAGAGCACACGTC447090.25083011139496025No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCGG441600.2477500664117167No Hit
TTGTGTAACTCCCTAAGGAAGGAGATCGGAAGAGCACACGTCTGAACTCC435450.2442997427966079Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAAAATCTTTGCTCTGAAGTGGATAGATCGGAAGAGCACACGTCTGAAC423070.23735421330798234Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GATACGATCCACTGAGATTCATCCTGTGTTTTTCGGTCTGTAGATCGGAA406100.22783356424320236No Hit
TGAAAATCTTTACTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC397730.22313775795727375Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAAGTTGAAGATTGTAGCAGAAGCAAAAGCAGATCGGAAGAGCACACGTC392610.22026529341916692No Hit
TCCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCT367090.20594785298704055TruSeq Adapter, Index 3 (97% over 37bp)
ACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG357640.20064613621260502Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGACCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTAT354090.1986544859957536TruSeq Adapter, Index 3 (97% over 37bp)
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC351190.1970275041284665Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAAGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC336040.1885279264424667Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGCAATGATGTCTTTAATATACGCTGATGTCCAGTGATAAGAAACATGC323570.18153190441313222No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT307830.172701320071374No Hit
CCGGACCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTA291530.16355655992076037TruSeq Adapter, Index 3 (97% over 37bp)
GGGGGACTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTA268830.15082121909751317TruSeq Adapter, Index 2 (97% over 36bp)
TTGTTTATGCCGTGAGGATTGGAGGAAAAGATCGGAAGAGCACACGTCTG265130.14874541464614685Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGAATGTTTACTCGTGAAGGAAAAAAAAAAGATCGGAAGAGCACACGTC260530.1461646847876915No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA244210.13700870407247587Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATC230180.12913747800418696No Hit
TTGTTTATGCCGTGAGGATTGGAGGAAAAAGATCGGAAGAGCACACGTCT226970.1273365773855692Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAAAATCTTTGCTCTGAAGTGGAGATCGGAAGAGCACACGTCTGAACTC225080.12627623403068206Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATGATTGAAATCTTCATGAACGACCCAAAAGGCCAAAAAAAAAAAAAAA224410.12590034511651574No Hit
TATTAATGCTGTAGGTGAAAGACAAGATCGGAAGAGCACACGTCTGAACT223630.12546274309703853Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGATGGACAGTGATTCAGAAAAGATCGGAAGAGCACACGTCTGAACTCC217800.12219194851556137Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACAACGGGGACTAGCTCCGATCTCTCAAGGAGATCGGAAGAGCACACGT216610.12152432492174357No Hit
TGAAAATCTTTGCTCTGAGGTGGAAGATCGGAAGAGCACACGTCTGAACT206730.11598136600836548Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TACAACGGGGACTAGCTCCGATCTCTCAAGAGATCGGAAGAGCACACGTC206010.11557742568269422No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC201410.11299669582423882No Hit
TGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGA196570.1102813191905597No Hit
TCTCGGTGGGACCTCCATTAGATCGGAAGAGCACACGTCTGAACTCCAGT196370.1101691135445399Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCG193410.10850846998344686No Hit
TTTAAGGTGTTACACGGTTATAACTGGAGAACCTGGTGTAGAAAAAAAAA183740.10308332699838955No Hit
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTTGAACT182540.10241009312227077No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)