FastQCFastQC Report
Wed 30 Apr 2025
1F2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17902750
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA265687414.840591529234334Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC5166492.8858639035902303No Hit
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTGATCGTCGG2580711.4415159682171732No Hit
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG2458101.37302928321068Illumina RNA PCR Primer (100% over 37bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCT1770730.9890826828280571Illumina DpnII expression Sequencing Primer (100% over 22bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1566110.8747873929982823Illumina RNA PCR Primer (100% over 45bp)
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG1562420.8727262571392663Illumina RNA PCR Primer (100% over 36bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT1547860.8645934283839075No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1350490.7543477957297063No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT1164900.6506821577690578Illumina DpnII expression Sequencing Primer (100% over 22bp)
AACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAA1117550.6242337071120359Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1041680.5818547429864127Illumina RNA PCR Primer (100% over 50bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATGATCGTCGGACTG965780.5394590216586838No Hit
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT876440.48955607378754656No Hit
CGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACGT637370.3560179302062532Illumina DpnII expression Sequencing Primer (96% over 28bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCGATCGTC630640.3522587312005139No Hit
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG562280.3140746533353814No Hit
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC511720.28583318205303654Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG511040.28545335213863793Illumina DpnII expression Sequencing Primer (100% over 23bp)
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA473250.2643448632193378Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCGATCGTCGGA470250.26266914300875566No Hit
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG466910.26080350784097417Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC466640.26065269302202176No Hit
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC428730.23947717529429835Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAGATCGTCG388320.21690522405775647No Hit
TCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTG346420.1935009984499588Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCT333630.18635684461884347Illumina DpnII expression Sequencing Primer (100% over 22bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTGATCGTCG315960.17648685257851449No Hit
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC308380.17225286617977686No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGATCGTCGGAC282110.1575791428691123No Hit
ACAGACCGAAAAACACAGGATGAATCTCAGTGGATCGTATCGATCGTCGG281350.15715462708243147No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA280980.15694795492312633No Hit
ACTCATCCTAAGTAGACTTTTCACATTGAAGATCGTCGGACTGTAGAACT279000.15584197958414212No Hit
AAGCTCAGAGCATGTTTCTTATCACTGGACATCAGCGTATATTAAAGACA274590.15337867087458631No Hit
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG274310.15322227032159863Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACAGATCG267030.14915585594391922No Hit
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTA250690.14002876653028165No Hit
ATCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA250600.1399784949239642Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACTCGTTCTCAAAGTAGTTCAGCAGAAGATCGTCGGACTGTAGAACTCTG250490.1399170518495762Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTTTTTTTTCCTTCACGAGTAAACATTCTAGATCGTCGGACTGTAGAACT246640.13776654424599571No Hit
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGAC231060.12906397061903896No Hit
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTGATCGTCGGACT226310.12641074695228385No Hit
AACATTCTTGGCAAATGCTTTCGCAGTAGTTCGTCTTTGATCGTCGGACT216300.12081942718297467No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG216280.12080825571490414No Hit
CCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAACG207520.11591515270000419Illumina Small RNA Adapter 1 (100% over 26bp)
CCACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGT191270.10683833489268407Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCACTGCAAGCAGTCCACCACGGTCGACGACCGGCCGGAAGGATCGTC188730.10541955844772452No Hit
AGTCAGGAGGCCTCACTAAACCATTCAATCGGTGATCGTCGGACTGTAGA182890.10215748977112456No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)