FastQCFastQC Report
Wed 30 Apr 2025
1F2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17902750
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC250525013.993660191869964Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG5065262.8293195179511526No Hit
AGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAA2477641.3839438075156052No Hit
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCAT2321191.2965549985337448TruSeq Adapter, Index 14 (97% over 41bp)
TCTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTG1676550.9364762396838474Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG1520580.8493555459356803No Hit
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCA1480190.826794766167209TruSeq Adapter, Index 14 (97% over 39bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1310950.7322618033542333No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG1114470.6225133010291716Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAAC1070220.5977964279230845Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGAGC907440.5068718492968958No Hit
GGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCG844800.4718828112999399No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTAGGGGG704130.3933082906257418TruSeq Adapter, Index 14 (97% over 43bp)
GAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGG605850.33841169652706987No Hit
TCTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCC596910.333418050299535Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTGGGGGG588890.3289382916032453TruSeq Adapter, Index 14 (97% over 43bp)
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA485860.2713884738378182Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT481610.26901453687282684Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC463250.2587591291840639No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT448300.2504084568013294No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA445980.24911256650514585Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAG444380.24821884905950206No Hit
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT439520.24550418231835894Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT411250.22971331220063956Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGA380180.21235843655304354No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAAGATCGGAAGAGCACACGTCTGA329740.18418399407912192Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTCCGAGGTCGCGGTCGGACCGGGAAAGATCGGAAGAGCACACGTCTG317290.1772297552052059Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCAATGATGTCTTTAATATACGCTGATGTCCAGTGATAAGAAACATGC313060.17486698970828504No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCGG306300.17109103350043986No Hit
AAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGA302530.16898521176914164No Hit
GATACGATCCACTGAGATTCATCCTGTGTTTTTCGGTCTGTAGATCGGAA276190.15427238832023013No Hit
TACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGAAGA272220.1520548519082264No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG270720.15121699180293532No Hit
TTCAATGTGAAAAGTCTACTTAGGATGAGTAGATCGGAAGAGCACACGTC265890.148519082263898No Hit
TGTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATC263770.14733490664841994No Hit
GATGATTGAAATCTTCATGAACGACCCAAAAGGCCAAAAAAAAAAAAAAA261080.14583234419293126No Hit
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA257620.14389968021672647Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCAGATCGGAAGAGCAC245220.13697337001298682No Hit
TGAAAATCTTTGCTCTGAAGTGGATAGATCGGAAGAGCACACGTCTGAAC244950.13682255519403444Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGAATGTTTACTCGTGAAGGAAAAAAAAAAGATCGGAAGAGCACACGTC241480.13488430548379438No Hit
TTCTGCTGAACTACTTTGAGAACGAGTAGATCGGAAGAGCACACGTCTGA237010.13238748237002695Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGA218000.12176900196897125No Hit
AATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGAG214210.11965200876960244No Hit
TTTAAGGTGTTACACGGTTATAACTGGAGAACCTGGTGTAGAAAAAAAAA210920.11781430227199732No Hit
AAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTAGATCGGAAGAG209410.11697085643267095No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAAAAAAAAGATCGGAAGAGCAC201320.11245199759813437No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTGGGGGGGGGGG194210.10848054069905462TruSeq Adapter, Index 14 (97% over 39bp)
CTTCCGGCCGGTCGTCGACCGTGGTGGACTGCTTGCAGTGCTCAGATCGG194100.1084190976246666No Hit
TGAAAATCTTTGCTCTGAAGTGGAGATCGGAAGAGCACACGTCTGAACTC193460.10806161064640908Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC182460.10191730320760777Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)