FastQCFastQC Report
Wed 30 Apr 2025
1E8_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E8_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11714767
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA166719414.231559193622886Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG2489652.1252236600181633Illumina Small RNA Adapter 1 (100% over 26bp)
CAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA2458652.0987613325984205Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG2219691.89477946936546Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT1633641.394513437612545Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC1627761.3894941316374454No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1561251.3327196349701194No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1222631.043665657200011Illumina RNA PCR Primer (100% over 50bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1209501.032457581102552Illumina RNA PCR Primer (100% over 45bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT1168220.9972200044610363Illumina DpnII expression Sequencing Primer (100% over 22bp)
CAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT1041270.8888525055598631Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC1008000.8604524528742228Illumina RNA PCR Primer (100% over 30bp)
CGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACGT969280.8274001523034986Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG814290.6950970514394353Illumina RNA PCR Primer (100% over 36bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG811690.6928776304300376Illumina DpnII expression Sequencing Primer (100% over 23bp)
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG706740.6032898477622304Illumina RNA PCR Primer (100% over 37bp)
GCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGT605220.5166299935798979Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG602890.5146410509060915Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC589650.5033390762274657Illumina DpnII expression Sequencing Primer (100% over 26bp)
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA581740.49658691461810556Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG545240.4654296581400211Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAG477540.40763934954916303Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG475780.4061369722504937Illumina Small RNA Adapter 1 (100% over 26bp)
CCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGT474650.4051723777348709Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG417770.3566182750369683Illumina DpnII expression Sequencing Primer (100% over 23bp)
GAGGTCTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG402440.34353222731617283Illumina RNA PCR Primer (100% over 36bp)
GGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT392500.335047210072552Illumina RNA PCR Primer (100% over 35bp)
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAG387000.33035228101421055No Hit
GGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTA386700.3300961939746646No Hit
AACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAA371480.31710404483503596Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT352360.3007827641813106Illumina RNA PCR Primer (100% over 31bp)
TTCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCT345020.2945171679470876Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC344500.2940732837452081Illumina DpnII expression Sequencing Primer (100% over 26bp)
GACCGCGACCTCGGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT339080.2894466445640788Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACT338950.28933567351360895No Hit
GGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC333080.28432490377316083Illumina RNA PCR Primer (100% over 32bp)
GAGGTCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG309240.26397452036391333Illumina RNA PCR Primer (100% over 37bp)
ATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCT305460.26074782366563504Illumina DpnII expression Sequencing Primer (100% over 22bp)
GAGCTAGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC294410.25131528437569434Illumina RNA PCR Primer (100% over 32bp)
TCAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA293860.25084579146986025Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG281910.24064499106128187Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCCAGTCCGGGCCGGATCCGATCGTCGGACTGTAGAACTCTGAACGTGT280970.23984258500403804Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC279410.2385109323983994No Hit
AGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA273530.23349162642329976Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC273340.23332943796492067Illumina DpnII expression Sequencing Primer (100% over 21bp)
CGCGACCTCGGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC270730.23110148072087136Illumina RNA PCR Primer (100% over 32bp)
ACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG263770.2251602614034065Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG255940.2184763896712585Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGT253550.21643622958954284Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCGTTCTCAAAGTAGTTCAGCAGAAGATCGTCGGACTGTAGAACTCTG236320.20172829728495664Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACGTG235770.2012588043791225Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT233910.19967106473393798No Hit
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG230620.19686264353358457No Hit
TCCAACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCT216320.1846558279818967Illumina DpnII expression Sequencing Primer (100% over 22bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGAC206850.1765720137668978No Hit
CGGTCCGACCGCGACCTCGGAAGATCGTCGGACTGTAGAACTCTGAACGT206690.1764354340124733Illumina DpnII expression Sequencing Primer (96% over 28bp)
GTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT199640.17041738858314467Illumina RNA PCR Primer (100% over 31bp)
ACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACG193660.16531272026152974Illumina Small RNA Adapter 1 (100% over 26bp)
GGCCAGTCCGGGCCGGATCGATCGTCGGACTGTAGAACTCTGAACGTGTA193010.1647578650091803Illumina DpnII expression Sequencing Primer (96% over 30bp)
TAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA189110.1614287334950836Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA187900.16039584910224847Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT187230.15982392138059598No Hit
GCCAGTCCGGGCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC185460.15831300784727517Illumina RNA PCR Primer (100% over 30bp)
GGCCAATCCACGGCCGATTCCTTCCCGGGATCGTCGGACTGTAGAACTCT184310.1573313408623492Illumina DpnII expression Sequencing Primer (100% over 22bp)
GTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC181230.15470218058967797Illumina RNA PCR Primer (100% over 30bp)
AGGGAGAGAGAACCCCCCCTCACCTCGACCACGGCCAGTCCGGATCGTCG179740.1534302816266No Hit
TCAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAG179000.15279860026238679Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTA178370.1522608174793404No Hit
GGCCAGTCCGGGCCGGATCCAAGATCGTCGGACTGTAGAACTCTGAACGT175120.14948654121759314Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGT171500.14639642427373928Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCAGTCCGGGCCGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT168990.14425382937620526Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC164670.14056617600674431No Hit
CCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAACG161690.13802237808058837Illumina Small RNA Adapter 1 (100% over 26bp)
GGCCAGTCCGGGCCGGATCCAGATCGTCGGACTGTAGAACTCTGAACGTG161410.13778336351034554Illumina DpnII expression Sequencing Primer (96% over 29bp)
ATCCAACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTC157800.1347017828011432Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG155410.13266162271942755No Hit
TGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG152750.13039098430212057Illumina Small RNA Adapter 1 (100% over 26bp)
ACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAAC152500.1301775784358323Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCCAGTCCGGGCCGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA152080.12981905658046805Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTA148640.12688259186034173Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTCATCCTAAGTAGACTTTTCACATTGAAGATCGTCGGACTGTAGAACT143570.12255472089201602No Hit
ATCCAACCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGT141640.12090722760427074Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG140710.12011335778167845Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCTTCCTTAGGGAGTTACACAAGATCGTCGGACTGTAGAACTCTGAACGT131220.1120124710973765Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGT130040.11100519540849596No Hit
CACCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGT128070.10932355718214455Illumina DpnII expression Sequencing Primer (96% over 28bp)
CACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC125660.10726632463112583Illumina RNA PCR Primer (100% over 32bp)
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC124800.10653220845109426No Hit
CGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC124120.10595174449479021Illumina RNA PCR Primer (100% over 30bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT124050.10589199085222949No Hit
GGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT123740.10562736757803208Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACGT119360.10188849680066193Illumina DpnII expression Sequencing Primer (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)