FastQCFastQC Report
Wed 30 Apr 2025
1E8_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E8_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11714767
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC72

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC156822113.386702441457011Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC2359122.0138001891117425Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2248331.9192272454074417Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA2055241.7544010905210492Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG1576391.3456434942325357No Hit
GAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1529281.305429292789178Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1515171.2933846656958692No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG1115480.952199902908867Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC954900.8151250468745985Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA946030.8075534067386915Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTGGGGGG938520.8011426945153924TruSeq Adapter, Index 13 (97% over 43bp)
TCTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCC900790.7689354811751697Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTGGGGGGGGGGG824170.703530851275147TruSeq Adapter, Index 13 (97% over 39bp)
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAA775060.6616094029014833TruSeq Adapter, Index 13 (97% over 39bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA772280.6592363296683579Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAAT667470.569768054285672TruSeq Adapter, Index 13 (97% over 41bp)
CTCGGGCTGAGACTTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG570780.4872312014400287Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA570530.4870177955737404Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT562500.4801631991485618Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT542620.4631931646613202Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGACTGGCCGTGGTCGAGGTAGATCGGAAGAGCACACGTCTGAACTCCA514310.43902708436283877Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTGGATCCGGCCCGGACTGGCTAGATCGGAAGAGCACACGTCTGAACTC459540.3922741271764091Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC438300.37414316477655934Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGACTGGCCGTGGTCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAG435830.37203471481763145Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA392970.3354484131011739Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAA389610.33258023825825983TruSeq Adapter, Index 13 (97% over 39bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCAGATCGGAAGAGCAC381110.3253244388044594No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCAGATCGGAAGAGCACA378120.3227721046436519No Hit
CGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAA370150.3159687256263825TruSeq Adapter, Index 13 (100% over 39bp)
TCTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAAC353290.30157663400390294Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGGATCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG333650.284811469148298TruSeq Adapter, Index 13 (100% over 35bp)
CTTTCCGAGGTCGCGGTCGGACCGGGAAAGATCGGAAGAGCACACGTCTG327000.2791348731050306Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACCAAGATAGTCGAACTCGACCCTGTCAAGATCGGAAGAGCACACGTC326210.27846051056755977No Hit
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT323640.27626669826211653Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC321090.2740899584259764Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTGGATCCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGT313040.26721828953149473TruSeq Adapter, Index 13 (100% over 36bp)
CAGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAAT300460.25647970633987TruSeq Adapter, Index 13 (97% over 41bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTG290660.24811419638137064Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGGCTGAGACTTGAAGCAAGATCGGAAGAGCACACGTCTGAACTCCA276420.23595859823759194Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGACTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGT275420.23510497477243894TruSeq Adapter, Index 13 (100% over 36bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT274130.23400380050239153No Hit
AGTGTGCTCTTAACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA272610.232706292835359Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCT267220.22810526235818432Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG261280.22303473897517553Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA258630.22077263679252007Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCCGAGGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGT251200.21443021444643331TruSeq Adapter, Index 13 (100% over 36bp)
CAGCTGTGACCCTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC249350.21285101103590023Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC242140.2066963858521471Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTTGGATCCGGCCCGGACTGGCAGATCGGAAGAGCACACGTCTGAACTCC238260.20338432680735347Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG227140.19389203387485215No Hit
TTCTGCTGAACTACTTTGAGAACGAGTAGATCGGAAGAGCACACGTCTGA224410.19156164181498445Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCGGGCTGAGACTTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCA222530.18995682970049682Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTG203690.1738745636170143Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGA198200.16918817079332435No Hit
TTCCGAGGTCGCGGTCGGACCGAGATCGGAAGAGCACACGTCTGAACTCC192880.1646468939587104Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTAGGGGG189480.1617445741771902TruSeq Adapter, Index 13 (97% over 43bp)
AGTGTGCTCTTAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG187730.16025073311317248TruSeq Adapter, Index 13 (100% over 35bp)
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC186470.1591751675470797No Hit
CTCGGGCTGAGACTTGAAGCGGTAGATCGGAAGAGCACACGTCTGAACTC184390.15739963073956145Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAAAATCTTTGCTCTGAAGTGGATAGATCGGAAGAGCACACGTCTGAAC183460.15660576091696915Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGTGTGCTCTTAACTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA182110.1554533692390126Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC181020.15452291966199583No Hit
GATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT179580.1532937018721755Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGACTGGCCGTGGTCGAGGTGAGGGGGGGTTCTCTCTCCCTAGATCGGA178150.15207302031700673No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTG177730.15171449846164248Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGCCCGGACTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA177310.1513559766062782Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCAGATCGGAAGAGCAC174110.14862438151778862No Hit
GAGTGTGCTCTTAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA169030.14428797431481138Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGTGTGCTCTTAACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC166430.1420685533054136Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCC166420.14206001707076205Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC162180.13844065357851335Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGGCCCGGACTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC160870.1373224068391629Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT157650.13457373928137026No Hit
TGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCA152560.1302287958437415Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCGGGCTGAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCT152120.12985320151907417Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAAAATCTTTGCTCTGAAGTGGAGATCGGAAGAGCACACGTCTGAACTC149300.12744598334734272Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTCGGGCTGAGACTTGAAGCGGTAGATCGGAAGAGCACACGTCTGAACT145690.12436440263814039Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTGGATCCGGCCCGGACTGGCAAGATCGGAAGAGCACACGTCTGAACTC145120.1238778372630032Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA144920.12370711256997258Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTTGGATCCGGCCCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGT139170.11879877764534284Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCAATGTGAAAAGTCTACTTAGGATGAGTAGATCGGAAGAGCACACGTC137390.1172793278773705No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA134620.1149147908788967Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTAAAC133680.11411238482165287Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
GAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTGAACTCCAG133270.11376239920094014Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCAGATCGGAAGAGCA127940.10921258613167467No Hit
GGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCG121480.10369817854678628No Hit
GAGTGTGCTCTTAACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC121330.10357013502701334Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGTGTAACTCCCTAAGGAAGGAGATCGGAAGAGCACACGTCTGAACTCC120070.10249456946092056Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCGG118760.10137632272157013No Hit
CGGACTGGCCGTGGTCGAGGTGAGATCGGAAGAGCACACGTCTGAACTCC118220.10091536605038752Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCGGGCTGAGACTTGAAGCAAGATCGGAAGAGCACACGTCTGAACTCC117480.10028368468617431Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)