FastQCFastQC Report
Wed 30 Apr 2025
1E6_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E6_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21624116
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA499746123.110590971672554Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC18176838.405814138251941No Hit
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG6574063.0401520228618826Illumina RNA PCR Primer (100% over 37bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT5879542.7189735756134494No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC4691832.169721065129321No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA4006421.8527555068609511Illumina RNA PCR Primer (100% over 45bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT2302681.0648666516587313Illumina DpnII expression Sequencing Primer (100% over 22bp)
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG2011310.9301235712941976Illumina RNA PCR Primer (100% over 36bp)
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG1753070.8107013484389374Illumina Small RNA Adapter 1 (100% over 26bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1636990.75702054132525Illumina RNA PCR Primer (100% over 50bp)
ACTCGTTCTCAAAGTAGTTCAGCAGAAAGATCGTCGGACTGTAGAACTCT910360.42099293215038247Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACTCGTTCTCAAAGTAGTTCAGCAGAAGATCGTCGGACTGTAGAACTCTG890820.41195672461246513Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC890720.41191047994748087Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC749920.3467979916496933No Hit
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA712840.329650469873543Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG653350.3021395186744281Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAGATCGTCG652160.30158920716111587No Hit
CAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTCG623800.2884742201715899No Hit
GAGCTAGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC577640.2671276828148721Illumina RNA PCR Primer (100% over 32bp)
CCACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGT553350.25589485369020404Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT544220.2516727157771444No Hit
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC527350.24387124079430578No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCGATCGTCGG524250.24243765617979482No Hit
AAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTCGG502150.2322175852182813No Hit
TTTTTTTTTCCTTCACGAGTAAACATTCTAGATCGTCGGACTGTAGAACT490300.22673759241765074No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACAGATCG453010.20949295684503355No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA444780.20568702091683191No Hit
TGCGTGAGCCTAGAGATCCACCGTCAAAAGTTGATCGTCGGACTGTAGAA441010.20394359704692666No Hit
CAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGTC416310.19252116479582332No Hit
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG404720.18716140812415177Illumina DpnII expression Sequencing Primer (100% over 23bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC404620.18711516345916754Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTTCTGAATCACTGTCCATCCTGATCGTCGGACTGTAGAACTCTGAACGT399220.18461795155001942Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAACTCTGAAC385580.17831017924617126Illumina DpnII expression Sequencing Primer (100% over 26bp)
CCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAACG382000.17665462023973605Illumina Small RNA Adapter 1 (100% over 26bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT373710.17282093751254385No Hit
AATGAGGTCAAAGTAGCGGATGAGGGGTGGATCGTCGGACTGTAGAACTC357830.1654772847130491Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGTTAAAGCTCACAAGAATGCGGGTGAAGCTTACATAGCATTTCTTCATC317180.14667882839696197No Hit
TTCCACTTCAGAGCAAGGATTTTCAGATCGTCGGACTGTAGAACTCTGAA284530.1315799452796128Illumina DpnII expression Sequencing Primer (100% over 25bp)
CAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA280700.12980877461071702Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG272630.12607683014649015No Hit
TGACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACT268520.12417617441563855No Hit
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATGATCGTCGGACTG262150.12123038925614346No Hit
TCCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA250780.1159723708474372Illumina DpnII expression Sequencing Primer (100% over 25bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGGCTGTGAGCAACCGCCTATT244230.1129433452909705No Hit
GGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT239680.11083921303418831Illumina RNA PCR Primer (100% over 31bp)
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT239130.11058486737677507No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACAAGGCT229210.10599739661034005No Hit
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG222030.10267702966447276Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGTCG219330.1014284237098987No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)