FastQCFastQC Report
Wed 30 Apr 2025
1E6_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E6_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21624116
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC477035022.06032376074934Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG17934448.29372169479668No Hit
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCAT6210942.872228395371168TruSeq Adapter, Index 3 (97% over 36bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG5789742.6774458664576164No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT4654422.152420935958723No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG2231771.0320745597184182Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCA1911440.883939024374453TruSeq Adapter, Index 3 (97% over 36bp)
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC1690530.7817799349578035Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTAGGGGG1418530.6559944462007141TruSeq Adapter, Index 3 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTGGGGGG1038580.48028784159315463TruSeq Adapter, Index 3 (97% over 36bp)
TTTCTGCTGAACTACTTTGAGAACGAGTAGATCGGAAGAGCACACGTCTG875250.40475643027442143Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCTGCTGAACTACTTTGAGAACGAGTAGATCGGAAGAGCACACGTCTGA857960.39676072769864906Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT847550.39194665807379137Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCGG747530.3456927441565704No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTAGTGGG691660.31985584982988435TruSeq Adapter, Index 3 (97% over 36bp)
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT666560.3082484389188441Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGA643790.29771852870193627No Hit
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA617300.2854683169476153Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGAGATCGGA613940.2839144962041454No Hit
GGGGGACTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCA546580.25276408987077204TruSeq Adapter, Index 3 (97% over 36bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC533490.2467106632243371No Hit
ACCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC531550.24581351672364318Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT517010.239089542434937No Hit
GCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGAA514550.2379519236763251No Hit
CTTGATGAAGAAATGCTATGTAAGCTTCACCCGCATTCTTGTGAGCTTTA496020.22938278725474834No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTAGATCGGAA495380.22908682139884934No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAAAGATCGGAAGAGCACACGTC484250.22393979018610516No Hit
TGTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATC447970.20716222572982868No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG436150.20169610632869336No Hit
AACTTTTGACGGTGGATCTCTAGGCTCACGCAAGATCGGAAGAGCACACG432110.19982782186333073No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGAGATCGG409260.18926091591443553No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCT399440.18471968981298473Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA389820.18027095304150237Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGATGGACAGTGATTCAGAAAAGATCGGAAGAGCACACGTCTGAACTCC379000.17526728029020933Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTAATGCTGTAGGTGAAAGACAAGATCGGAAGAGCACACGTCTGAACT369920.17106826470964176Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCG360660.16678600873210261No Hit
TGAAAATCTTTGCTCTGAAGTGGAGATCGGAAGAGCACACGTCTGAACTC359330.16617095468781243Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACCCCTCATCCGCTACTTTGACCTCATTAGATCGGAAGAGCACACGTCT349690.16171296898333323Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTGGTGGG347140.16053373002623553TruSeq Adapter, Index 3 (97% over 36bp)
CCTGTGAATAGGCGGTTGCTCACAGCCCGACCAAGATAGTCGAACTCGAC338570.1565705622370875No Hit
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTTGAACT314880.14561520110232484No Hit
GATGATTGAAATCTTCATGAACGACCCAAAAGGCCAAAAAAAAAAAAAAA280640.1297810278117265No Hit
TGAAAATCCTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC275840.12756128389248372Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA262220.12126276052163243Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGACCAAGATAGTCGAACTCGACCCTGTCAAGATCGGAAGAGCACACGTC261820.12107778186169552No Hit
ATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGAGC251740.11641631963128574No Hit
TGAAAATCTTTGCTCTGAAGTGGGAAGATCGGAAGAGCACACGTCTGAAC236970.10958598261311583Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCG236560.10939637948668053No Hit
GTTGGATCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC229780.10626099120075011TruSeq Adapter, Index 5 (97% over 35bp)
TTTAAGGTGTTACACGGTTATAACTGGAGAACCTGGTGTAGAAAAAAAAA226080.10454993859633384No Hit
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC218060.10084111646459908No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTAGATCGGA216400.10007345502586094No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)