FastQCFastQC Report
Wed 30 Apr 2025
1E4_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E4_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18793445
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA559028729.74594067239934Illumina DpnII expression Sequencing Primer (100% over 25bp)
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG12810026.816217037376596Illumina RNA PCR Primer (100% over 37bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC7177793.819305082170938No Hit
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG5637952.999955569614831Illumina RNA PCR Primer (100% over 36bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC2241031.1924530068861776No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1961721.04383203824525Illumina RNA PCR Primer (100% over 45bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT1755020.9338468811864987No Hit
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC1123620.5978786752508655Illumina DpnII expression Sequencing Primer (100% over 26bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTGATCGTCGG1116740.5942178243531189No Hit
CGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACGT1087480.5786485660292724Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1038060.5523521632143548Illumina RNA PCR Primer (100% over 50bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT987900.5256620060877609Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACTCATCCTAAGTAGACTTTTCACATTGAAGATCGTCGGACTGTAGAACT762820.40589684328764636No Hit
ACTCGTTCTCAAAGTAGTTCAGCAGAAGATCGTCGGACTGTAGAACTCTG681090.36240827586427077Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCT636020.3384265098815039Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG618280.32898704840969817Illumina DpnII expression Sequencing Primer (100% over 23bp)
AACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAA617170.32839641694218386Illumina DpnII expression Sequencing Primer (100% over 25bp)
GAGCTAGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC560910.298460447246367Illumina RNA PCR Primer (100% over 32bp)
AAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG557380.29658213275958717Illumina RNA PCR Primer (100% over 37bp)
CCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAACG533310.2837744756216862Illumina Small RNA Adapter 1 (100% over 26bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCT506380.2694450112791987Illumina DpnII expression Sequencing Primer (100% over 22bp)
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC489660.2605482922370007No Hit
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG485220.25818576636694335Illumina DpnII expression Sequencing Primer (96% over 29bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT478640.25468454559555204No Hit
TCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTG472890.2516249681737436Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGT461370.24549517132170287Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGACCGAAAAACACAGGATGAATCTCAGTGGATCGTATCGATCGTCGG453990.2415682702133643No Hit
CCTTCCTTAGGGAGTTACACAAGATCGTCGGACTGTAGAACTCTGAACGT453560.24133946703225514Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC419670.22330658375832638No Hit
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG379960.20217687603310622No Hit
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATGATCGTCGGACTG347480.18489425435304704No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAGATCGTCG343260.18264879057565017No Hit
GCAGAAATACTCACCACTATACTACAGCGGAGATCGTCGGACTGTAGAAC304290.16191283716210625No Hit
ATCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA301330.16033781991540136Illumina DpnII expression Sequencing Primer (100% over 25bp)
TTCCACTTCAGAGCAAGGATTTTCAGATCGTCGGACTGTAGAACTCTGAA300280.15977911447315807Illumina DpnII expression Sequencing Primer (100% over 25bp)
AATAGGCTTCCGCCGTTTAGAGTCTTCGAGATCGTCGGACTGTAGAACTC299650.15944389120781208Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA292570.15567662022582873Illumina DpnII expression Sequencing Primer (100% over 25bp)
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC285870.15211154740389535Illumina DpnII expression Sequencing Primer (100% over 26bp)
AATGAGGTCAAAGTAGCGGATGAGGGGTGGATCGTCGGACTGTAGAACTC273230.14538579808012847Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGTGT270880.14413536209034586Illumina DpnII expression Sequencing Primer (96% over 30bp)
CTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA265240.1411343157148676Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA258360.13747346481712108Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA257760.13715420456441063No Hit
AAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG251720.13394031802045872Illumina RNA PCR Primer (100% over 36bp)
ACGGGATCGGTCACGGTAAGATCGTCGGACTGTAGAACTCTGAACGTGTA245430.1305934063712108Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCGATCGTCGGA241050.1282628065264245No Hit
TTTCTGAATCACTGTCCATCCTGATCGTCGGACTGTAGAACTCTGAACGT238080.12668246827550778Illumina DpnII expression Sequencing Primer (96% over 28bp)
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA234540.12479883278451609Illumina DpnII expression Sequencing Primer (100% over 25bp)
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA231240.12304290139460859Illumina DpnII expression Sequencing Primer (96% over 30bp)
TAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG203560.10831436173623303Illumina RNA PCR Primer (100% over 37bp)
TTCCACTCCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA198460.10560064958819418Illumina DpnII expression Sequencing Primer (100% over 25bp)
TTCCACTTCAGAGCAAAGGTTTTCAGATCGTCGGACTGTAGAACTCTGAA197250.10495680807856143Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCGATCGTCGG197050.10485038799432463No Hit
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG196890.10476525192693516Illumina Small RNA Adapter 1 (100% over 26bp)
GAGGTCCGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT196220.10440874464474183Illumina RNA PCR Primer (100% over 35bp)
TTTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGA196100.10434489259419974Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC189950.10107247500391758No Hit
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCGATCGTC188770.10044459650692036No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)