FastQCFastQC Report
Wed 30 Apr 2025
1E4_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E4_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18793445
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC531168728.263508898980472Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAAT11942716.354721021079424TruSeq Adapter, Index 23 (97% over 38bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG7108723.782552906079753No Hit
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAA5299132.819669304909238TruSeq Adapter, Index 23 (97% over 38bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2220371.1814598121845143No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG1733770.9225397472363369No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTGGGGGG1540570.8197379458635711TruSeq Adapter, Index 23 (97% over 38bp)
AGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAA1089410.5796755198421577No Hit
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT1061470.5648086340742743Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCC1024100.5449240413346249Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG956790.5091083619847239Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCAATGTGAAAAGTCTACTTAGGATGAGTAGATCGGAAGAGCACACGTC734690.3909288584397379No Hit
TTCTGCTGAACTACTTTGAGAACGAGTAGATCGGAAGAGCACACGTCTGA653630.3477967982985557Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTCCGAGGTCGCGGTCGGACCGGGAAAGATCGGAAGAGCACACGTCTG609390.32425667566537164Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA596050.3171584560467759Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAAC595430.3168285537856418Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGACTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAA525560.27965069735750947TruSeq Adapter, Index 2 (97% over 36bp)
CGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAAT509560.27113709061856406TruSeq Adapter, Index 23 (97% over 38bp)
TGAAAATCTTTGCTCTGAAGTGGAGATCGGAAGAGCACACGTCTGAACTC502460.2673591776281571Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTG483620.25733440569304883Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT478150.2544238163891719No Hit
GGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCG463020.2463731370166566No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAAGATCGGAAGAGCACACGTCTGA455770.24251540896307197Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA455540.24239302586619962Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GATACGATCCACTGAGATTCATCCTGTGTTTTTCGGTCTGTAGATCGGAA451550.24026994518567513No Hit
ACCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC440370.23432106247683698Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGTGTAACTCCCTAAGGAAGGAGATCGGAAGAGCACACGTCTGAACTCC422260.22468472384919316Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTTGAACT416650.22169964048635044No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCGG400840.21328713282742998No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTGGGGGGGGGGG392110.20864189615049292TruSeq Adapter, Index 23 (97% over 34bp)
TGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGA340140.18098863726155584No Hit
ATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGAGC335750.17865271641255767No Hit
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACTCTGAACT315610.16793621392991012No Hit
TGAAAATCTTTGCTCTGAAGTGGATAGATCGGAAGAGCACACGTCTGAAC294360.15662907997974826Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAAATCCTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC290260.1544474682528935Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGAAGACTCTAAACGGCGGAAGCCTATTAGATCGGAAGAGCACACGTCT290010.15431444314759749Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC282980.15057377718667334No Hit
TGAAAATCTTTGCTCTGAAGTGGGAAGATCGGAAGAGCACACGTCTGAAC276090.14690760528471494Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT275160.14641275189301375Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACCCCTCATCCGCTACTTTGACCTCATTAGATCGGAAGAGCACACGTCT270370.14386399087554197Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG257860.13720741460652902Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG253380.13482360471962432No Hit
TCCGCTGTAGTATAGTGGTGAGTATTTCTGCAGATCGGAAGAGCACACGT251910.1340414171004837No Hit
AGTGTGCTCTTAACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA242290.1289226110486928Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGAAAATCTTTGCTCTGAAGTGGAGAGATCGGAAGAGCACACGTCTGAAC237010.12611312082484077Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAG234170.12460195562867799No Hit
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACAGTCTGAACT233690.12434654742650962No Hit
TTACCGTGACCGATCCCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT232350.12363353286212293Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAA232300.12360692784106372TruSeq Adapter, Index 23 (97% over 38bp)
AGGATGGACAGTGATTCAGAAAAGATCGGAAGAGCACACGTCTGAACTCC224320.1193607664800147Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC220680.11742392094690461Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTAACT218050.11602449683919049Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT215790.11482194988731444Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGCAATGATGTCTTTAATATACGCTGATGTCCAGTGATAAGAAACATGC203920.10850591788785931No Hit
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCAACGTCTGAACT196210.10440342364052997No Hit
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCGAACT195300.10391921225725245No Hit
GCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGAA195000.10375958213089723No Hit
TGAAAACCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC189490.10082770881017292Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC188530.10031689240583617Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAAAATCTTTGCTCTGGAGTGGAAAGATCGGAAGAGCACACGTCTGAAC187960.10001359516576126Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)