FastQCFastQC Report
Wed 30 Apr 2025
1D11_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D11_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12285541
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC70

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG3823183.1119345904262583Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGT2792102.272671590123707Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG2767792.25288410172576Illumina DpnII expression Sequencing Primer (96% over 29bp)
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA2583732.103065709519833Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG2111771.7189068027203684Illumina Small RNA Adapter 1 (100% over 26bp)
ATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCT2082501.6950820480758644Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG1942721.5813060247000927Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG1675011.3633994628319583Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA1088210.8857648189851793Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCGATCGTCGGACTGT943990.7683747911467635No Hit
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC899050.7317952054370256Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTA896080.7293777294789053No Hit
CGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC874600.7118937619434097Illumina RNA PCR Primer (100% over 30bp)
AATCCACGGCCGATTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT811110.6602151260575337Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA803110.6537034063050216Illumina DpnII expression Sequencing Primer (100% over 25bp)
GACAGGGTCGAGTTCGACTATCTTGATCGGATCGTCGGACTGTAGAACTC759280.6180273217109445Illumina DpnII expression Sequencing Primer (100% over 21bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC734100.5975316837899121Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC695210.5658765861430115No Hit
GGCCAATCCACGGCCGATTCCTTCCCGGGATCGTCGGACTGTAGAACTCT688840.5606916292900735Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGAAC677320.5513147528464558Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAG606930.4940197586740381No Hit
AGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT603510.491235998479839Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCTCGACCACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG548600.44654118202853255Illumina RNA PCR Primer (100% over 34bp)
CAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT498140.4054685096895611Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCAATCCACGGCCGATTCCTTCCGATCGTCGGACTGTAGAACTCTGAA484780.3945939377028655Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGAACACCGCCCCAACGACCGGCAGCAGTCGCGGGATCGTCGGACTGTAG449090.36554352795696987No Hit
ATCCACGGCCGATTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC437880.35641898065376204Illumina RNA PCR Primer (100% over 30bp)
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCCAGTGATCGTCGGA418420.34057922235577576No Hit
GGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTA410260.33393726820821323No Hit
TCCAACCGCTTCAAGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA394670.32124755434050484Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC380950.31007995496494617Illumina RNA PCR Primer (100% over 30bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGAC374120.3045205742262388No Hit
ATCCAACCGCTTCAAGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAG364190.29643790208343285Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG356520.2901947907707117Illumina DpnII expression Sequencing Primer (96% over 29bp)
ATCCAACCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGT353900.2880622025517639Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT350260.2850993700643708Illumina RNA PCR Primer (100% over 35bp)
GCCTCGACCACGGCCAGTCCGATCGTCGGACTGTAGAACTCTGAACGTGT341530.27799345588444174Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCAACCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGTA341340.2778388025403195Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTAGAACTCTGA340370.2770492565203274Illumina DpnII expression Sequencing Primer (100% over 24bp)
GGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT339250.2761376157549757Illumina RNA PCR Primer (100% over 44bp)
GACAGGGTCGAGTTCGACTATCTTGATCGGGATCGTCGGACTGTAGAACT336350.27377711734469No Hit
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG334290.27210034950841805Illumina RNA PCR Primer (100% over 37bp)
GCCTCGACCACGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG315230.25658617719805743Illumina RNA PCR Primer (100% over 33bp)
AATCCACGGCCGATTCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA311720.25372916015664265Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC292710.23825568609473524Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCCTCGACCACGGCCAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA280050.22795088958638454Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG257820.2098564483240909Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC247100.20113074385572438No Hit
ACTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGT242610.19747604114462686Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAA236410.19242945833642977Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG229800.18704914989091648Illumina DpnII expression Sequencing Primer (96% over 29bp)
AATCCACGGCCGATTCCTTCCGATCGTCGGACTGTAGAACTCTGAACGTG226450.18432236724455195Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCCAGTAACGTAGATC224220.18250722536353914No Hit
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT223600.18200256708271945No Hit
GGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG219390.1785757745629598Illumina Small RNA Adapter 1 (100% over 26bp)
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCCAGGATCGTCGGAC203850.16592675894370462No Hit
CAGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAG203740.1658372227971076Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT203460.16560931260576967Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGAACG201470.16398952231733221Illumina Small RNA Adapter 1 (100% over 26bp)
GGTCTCGGCGAGGAAGAACAGCTCGGCGATCGTCGGACTGTAGAACTCTG197010.1603592385553066Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACCCGATCTACATAGAACGACTACCCCCAGATCGTCGGACTGTAGAACTC193130.15720105447533814Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCTCGCCTCGACCACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC192920.15703012183183468Illumina RNA PCR Primer (100% over 30bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT191270.155687079632879Illumina RNA PCR Primer (100% over 50bp)
ACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGACT190200.1548161371159805No Hit
AACAGGGTCGAGTTCGACTATCTTGATCGGATCGTCGGACTGTAGAACTC188790.15366844650960018Illumina DpnII expression Sequencing Primer (100% over 21bp)
AACAGGGTCGAGTTCGACTATCTTGATCGGGATCGTCGGACTGTAGAACT188540.15346495526733417No Hit
TCCAACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCT187180.1523579629094071Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGCACACTCAGTTAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT180300.1467578839222465Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATCCACGGCCGATGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT179140.1458136845581322Illumina RNA PCR Primer (100% over 31bp)
GCCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG178190.14504041783752136Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACG176450.14362411879134993Illumina Small RNA Adapter 1 (100% over 26bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGGATCGTCGGACTGTAGAACT175770.1430706226123864No Hit
TTCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCT173330.14108454808787013Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCCAGTCCGGGCCGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA172000.14000197467901493Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGT168130.13685193024873712Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTCT160130.1303402104962248Illumina DpnII expression Sequencing Primer (100% over 22bp)
GACCGCGGTCCGTTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA156730.12757272960140706Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG150310.12234707450001592Illumina Small RNA Adapter 1 (100% over 26bp)
GATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTC149270.12150055093218931Illumina DpnII expression Sequencing Primer (100% over 21bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA148970.1212563614414701Illumina RNA PCR Primer (100% over 45bp)
ATCCAACCGCTTCAAGTCTGATCGTCGGACTGTAGAACTCTGAACGTGTA143210.11656792321966122Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCCGATCTGACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCT143100.11647838707306418Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTG143010.11640513022584842Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACCGCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGA142310.1158353547475036Illumina DpnII expression Sequencing Primer (100% over 24bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATGATCGTCGGACTG138410.11266089136815383No Hit
ATCCACGGCCGATGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC134310.10932363499499126Illumina RNA PCR Primer (100% over 32bp)
AATCCACGGCCGATTCCTTCGATCGTCGGACTGTAGAACTCTGAACGTGT132470.10782593945191345Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCGCGGTCCGTTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT131040.10666196954615184Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGTCGGCGCCAGTCAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA130250.10601893722059126Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCTCTTGTCCCCCATGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT128180.1043340297346287Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGCTATCCCACTACTGTTGATCGTCGGACTGTAGAACTCTGAACGTGTAG127650.10390262830102476Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATCCACGGCCGATTCCTTCCGATCGTCGGACTGTAGAACTCTGAACGTGT127270.10359332161278043Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGGGGACGAGCGCGAACGCAGATCGTCGGACTGTAGAACTCTGAACGTG126410.10289331173938535Illumina DpnII expression Sequencing Primer (96% over 29bp)
TGGGCCAATCCACGGCCGATTCCTTCCGATCGTCGGACTGTAGAACTCTG125610.10224213976413413Illumina DpnII expression Sequencing Primer (100% over 23bp)
GTCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCT125550.10219330186599027Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG125390.10206306747094003Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGACAGGGTCGAGTTCGACTATCTTGATCGGGATCGTCGGACTGTAGAAC124970.10172120218393313No Hit
AGTGTCGGCGCCAGTCAGGGATCGTCGGACTGTAGAACTCTGAACGTGTA124740.10153399024104841Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGATCGTCGGACTGTAG124500.10133863864847303No Hit
ATCCACGGCCGATTCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT123590.10059793052662476Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCTACATAGAACGACTACCCCCAGATCGTCGGACTGTAGAACTCTGAA123450.1004839754309558Illumina DpnII expression Sequencing Primer (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)