FastQCFastQC Report
Wed 30 Apr 2025
1D11_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D11_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12285541
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC71

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA3626152.95155907257157Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGGCTGAGACTTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG2607052.1220473725984066Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA2550112.0757002072599Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT2395161.9495763353034272Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTGGTGGACTGCTTGCAGTGCTAGATCGGAAGAGCACACGTCTGAACTC2007121.6337253687078168Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTG2000741.628532272205188Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGCTGTGACCCTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC1822301.4832883631254008Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA1567911.2762238146451994Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA988330.804465997875063Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCA930640.7575083588097585No Hit
CCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCAC889140.7237288125936009No Hit
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT859340.6994726565154925Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTTCCGAGGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC808650.6582127722336363Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC757010.6161796212311692Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAATCGGCCGTGGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC755680.615097047822314Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGATCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCT735810.5989235638870115Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCT712190.5796977113177189Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT681250.5545136351748775No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTG661540.5384703856346252Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCTGCCGGTCGTTGGGGCGAGATCGGAAGAGCACACGTCTGAACT627470.5107385991386134Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCAGATCGGAAGAGCACA603860.4915208862190114No Hit
GAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC559160.45513665210184884Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAAC506720.4124523291241305TruSeq Adapter, Index 2 (97% over 36bp)
GGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTGAAC459530.37404132223399844Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC453030.3687505499350822Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGCGACTGCTGCCGGTCGTTGGGGCGGTGTTCTAGATCGGAAGAGCACA437310.3559550206213955No Hit
ACTGGCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAG423350.3445920696532615No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCAGATCGGAAGAGCAC409490.3333105151820339No Hit
GAATCGGCCGTGGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC409090.33298492919440825Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA365030.2971216326574467Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGA364220.29646232103250475No Hit
AGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC354570.2886075590810368Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGGGCTGAGACTTGAAGCAAGATCGGAAGAGCACACGTCTGAACTCCA344180.2801504630524614Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC339740.27653645858981707Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTGAACTCCAG330360.26890146717999636Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGATCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTC329040.26782703342083186No Hit
CCGAGCTGTTCTTCCTCGCCGAGACCAGATCGGAAGAGCACACGTCTGAA327900.26689911335609884Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACA324820.2643921012513816TruSeq Adapter, Index 2 (97% over 36bp)
GGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAG317790.2586699275188614Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT316170.2573513042689777Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAAT309930.25227216286201803TruSeq Adapter, Index 2 (97% over 36bp)
CGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAA292710.23825568609473524TruSeq Adapter, Index 2 (97% over 36bp)
GGAATCGGCCGTGGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA290070.23610681857640622Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTGGGGG285340.23225676427273328TruSeq Adapter, Index 2 (97% over 36bp)
CGACCAAGATAGTCGAACTCGACCCTGTTAGATCGGAAGAGCACACGTCT283700.23092186172346826Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA265370.21600188384052443Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG244810.1992667640765677No Hit
CTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC242100.19706091901040418Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCGGGCTGAGACTTGAAGTAGATCGGAAGAGCACACGTCTGAACTCCAG229150.18652007266102485Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TACGTTACTGGCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGAT226460.1843305068942426No Hit
CTCGGGCTGAGACTTGAAGCGGTTGAGATCGGAAGAGCACACGTCTGAAC221420.18022812345015982Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCG219580.178730427907082No Hit
CGGACTGGCCGTGGTCGAGGTAGATCGGAAGAGCACACGTCTGAACTCCA218980.17824204892564358Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGTGGTGGACTGCTTGCAGTGCCAGATCGGAAGAGCACACGTCTGAACTC217940.17739552535781694Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGAAGGAATCGGCCGTGGATTAGATCGGAAGAGCACACGTCTGAACTCCA210720.17151869828117458Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGGCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGA204360.1663418810779273No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG194710.1584871191264593Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCTGCCGGTCGTTGGGGCAGATCGGAAGAGCACACGTCTGAACTC189140.15395333424877258Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGAGCTGTTCTTCCTCGCCGAGACCAGATCGGAAGAGCACACGTCTGA186700.15196725972425634Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC186530.15182888567951547Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGGGGTAGTCGTTCTATGTAGATCGGGTAGATCGGAAGAGCACACGTCT186040.1514300428446741Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTAGATCGGAAGAG185980.15138120494653023No Hit
CCGATCAAGATAGTCGAACTCGACCCTGTTAGATCGGAAGAGCACACGTC184210.14994048695128687No Hit
CGATCAAGATAGTCGAACTCGACCCTGTTAGATCGGAAGAGCACACGTCT182600.14863000335109378Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTG176920.14400668232681002Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGTCGAGGCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC176640.1437787721354721Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAGCTGTGACCCTCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC173440.14117408423446717Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTC171650.13971708693984253No Hit
CTTAACTGAGTGTGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC169600.13804845875326127Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGGGCTGAGACTTGAAGCGGTAGATCGGAAGAGCACACGTCTGAACTC168220.1369251870959529Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGGCCGTGGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC166300.13536237435534992TruSeq Adapter, Index 16 (100% over 35bp)
CTTTCCGAGGTCGCGGTCGGACCGGGAAAGATCGGAAGAGCACACGTCTG165440.13466236448195484Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA161870.13175650954239623Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCC155680.1267180663838898Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACCAAGATAGTCGAACTCGACCCTGTAGATCGGAAGAGCACACGTCTG154640.1258715428160632Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGAACGGACCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA148030.1204912343705499Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCAGATCGGAAGAGCACACGTCT142860.11628303548048881Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC142160.11571326000214398Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGCTGTGACCCTCGGGTCAGATCGGGAAGATCGGAAGAGCACACGTCTG135060.10993410872178931Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGAGC134890.10979573467704842No Hit
AGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTGAACTCCAGT134310.10932363499499126Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTTTCCGAGGTCGCGGTCGGAAGATCGGAAGAGCACACGTCTGAACTCCA134230.10925851779746613Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGACTGCTGCCGGTCGTTGGGGCGGTAGATCGGAAGAGCACACGTCTGAA134120.1091689816508691Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTGGGGGG125940.10251074820392524TruSeq Adapter, Index 2 (97% over 36bp)
ATCGGCCGTGGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCA125600.10223400011444346TruSeq Adapter, Index 23 (100% over 36bp)
CTCGGGCTGAGACTTGAAGCGGTTGGACAGATCGGAAGAGCACACGTCTG124630.10144445409445135Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAAGGAATCGGCCGTGGATTAGATCGGAAGAGCACACGTCTGAACTCCAG123800.1007688631701282Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCACA123030.10014211014394889No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)