FastQCFastQC Report
Wed 30 Apr 2025
1C9_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C9_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11672498
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA162713213.939878164896665Illumina DpnII expression Sequencing Primer (100% over 25bp)
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG3556353.0467771337377823Illumina RNA PCR Primer (100% over 37bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC2602602.229685539462076No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC2578612.2091329550881054No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA2310201.9791821767714162Illumina RNA PCR Primer (100% over 45bp)
GAGGTCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG1717491.4713988385348191Illumina RNA PCR Primer (100% over 37bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1520061.3022576658398228Illumina RNA PCR Primer (100% over 50bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT1501781.2865969220984232Illumina DpnII expression Sequencing Primer (100% over 22bp)
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG1369011.1728509184580713Illumina RNA PCR Primer (100% over 36bp)
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA1144690.9806726889137184Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT1046910.8969031307608705No Hit
GAGGTCTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG778730.6671493968129186Illumina RNA PCR Primer (100% over 36bp)
GAGCTAGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC516730.4426901593814795Illumina RNA PCR Primer (100% over 32bp)
ACTCGTTCTCAAAGTAGTTCAGCAGAAGATCGTCGGACTGTAGAACTCTG515150.4413365502397173Illumina DpnII expression Sequencing Primer (100% over 23bp)
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA514710.440959595795176Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG470360.40296430121470145No Hit
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG435010.37267943845439083Illumina Small RNA Adapter 1 (100% over 26bp)
ACTCATCCTAAGTAGACTTTTCACATTGAAGATCGTCGGACTGTAGAACT432280.3703406074689411No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC428320.366948017468069No Hit
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC409510.35083321496392633Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCCAGTCCGGGCCGGATCCGATCGTCGGACTGTAGAACTCTGAACGTGT373380.3198801147792015Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG371570.3183294612687019Illumina DpnII expression Sequencing Primer (100% over 23bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGAC368420.3156308101316445No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA360300.3086742872005633No Hit
CCACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGT356740.30562438305836503Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC337770.28937250621075283Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT324800.2782609172432499No Hit
AGGGAGAGAGAACCCCCCCTCACCTCGACCACGGCCAGTCCGGATCGTCG324770.27823521580384936No Hit
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC316000.2707218283524229No Hit
TTCCACTTCAGAGTAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA286180.24517459758827972Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG270460.2317070433423934Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTCGTTCTCAAAGTAGTTCAGCAGAAAGATCGTCGGACTGTAGAACTCT259560.22236885369352816Illumina DpnII expression Sequencing Primer (100% over 22bp)
AAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG251080.21510391348964036Illumina RNA PCR Primer (100% over 37bp)
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG239230.2049518449264245Illumina DpnII expression Sequencing Primer (96% over 29bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT233870.20035985442019352No Hit
ATCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA226940.19442282191866728Illumina DpnII expression Sequencing Primer (100% over 25bp)
AATGAGGTCAAAGTAGCGGATGAGGGGTGGATCGTCGGACTGTAGAACTC226250.19383168881245472Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGT225790.19343760007497968Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG225640.19330909287797693Illumina Small RNA Adapter 1 (100% over 26bp)
ACCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG221400.18967662277603303Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAAGGGAGAGAGAACCCCCCCTCACCTCGACCACGGCCAGTCCGGATCGT211370.18108377486978366No Hit
ACAGACCGAAAAACACAGGATGAATCTCAGTGGATCGTATCGATCGTCGG203330.1741957891104372No Hit
CCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAACG193260.16556867261832042Illumina Small RNA Adapter 1 (100% over 26bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC192440.16486616660803885Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC189790.1625958727943239No Hit
AGATTAAGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGG180290.1544570836508175No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG180210.1543885464790827No Hit
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG171890.14726068061866449Illumina DpnII expression Sequencing Primer (100% over 23bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATGATCGTCGGACTG169600.1452988040777561No Hit
GACCGCGACCTCGGAAAGGGAGATCGTCGGACTGTAGAACTCTGAACGTG169290.1450332225372838Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCGACAGTCGCCGCAGTGAAAACACGCACAGATCGTCGGACTGTAGAACT164310.14076678359679307No Hit
CGCGACCTCGGAAAGGGAGAGAGAACCCCCCCTCACCTCGACCACGGCCA161510.1383679825860754No Hit
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG160560.13755410367172477Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGTGT157110.1345984381406619Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCT156330.1339302007162477Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCCAGTCCGGGCCGGATCCGATCGTCGGACTGTAGAACTCTGAACGTGTA153380.1314028925085273Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAACTAACCAGCCTAGACTTGAGATCGTCGGACTGTAGAACTCTGAACGT153100.13116301240745554Illumina DpnII expression Sequencing Primer (96% over 28bp)
TTTTTTTTTCCTTCACGAGTAAACATTCTAGATCGTCGGACTGTAGAACT145260.12444636957744605No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC140860.12067682513203258No Hit
AGGGAGAGAGAACCCCCCCTCACCTCGACCACGGATCGTCGGACTGTAGA140190.12010282631875371No Hit
CGCGACCTCGGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC135620.11618764038340379Illumina RNA PCR Primer (100% over 32bp)
ACCGTCAAAAGTTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC132100.113172004827073Illumina RNA PCR Primer (100% over 32bp)
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAG130640.11192120144291307No Hit
CCTTCCTTAGGGAGTTACACAAGATCGTCGGACTGTAGAACTCTGAACGT127700.10940246038165952Illumina DpnII expression Sequencing Primer (96% over 28bp)
TAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG127300.10905977452298557Illumina RNA PCR Primer (100% over 37bp)
CGGTCCGACCGCGACCTCGGAAGATCGTCGGACTGTAGAACTCTGAACGT126570.10843437283090561Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTC126030.10797174692169575Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCCAATCCACGGCCGATTCCTTCCCGGGATCGTCGGACTGTAGAACTCT125230.10728637520434786Illumina DpnII expression Sequencing Primer (100% over 22bp)
GACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG119110.10204328156663638Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGGGATCGGTCACGGTAAGATCGTCGGACTGTAGAACTCTGAACGTGTA117640.1007839110360096Illumina DpnII expression Sequencing Primer (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)