FastQCFastQC Report
Wed 30 Apr 2025
1C9_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C9_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11672498
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC149428312.801741323922267Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAAT3271542.802776235215461TruSeq Adapter, Index 19 (97% over 41bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG2551772.186138733971083No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2448562.0977172152867363No Hit
CAGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAAT1616791.3851276736136515TruSeq Adapter, Index 19 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTGGGGGG1609841.3791735068191915TruSeq Adapter, Index 19 (97% over 43bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG1400841.200120145662051Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAA1273151.0907262524268584TruSeq Adapter, Index 19 (97% over 39bp)
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC1048940.8986422614936408Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG1026820.8796917335089712No Hit
CCAGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAA733110.6280660746311544TruSeq Adapter, Index 19 (97% over 39bp)
TTCTGCTGAACTACTTTGAGAACGAGTAGATCGGAAGAGCACACGTCTGA478890.41027207715092345Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGACTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTG474270.40631405548323934TruSeq Adapter, Index 19 (100% over 36bp)
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT468120.40104526040612726Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT411960.3529321658483043No Hit
TTCAATGTGAAAAGTCTACTTAGGATGAGTAGATCGGAAGAGCACACGTC404210.3462926273364964No Hit
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC403220.3454444798362784Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT382700.3278646952863046Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC370480.31739564230381534No Hit
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGA349880.2997473205821068No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTGGGGGGGGGGG347460.29767407113712935TruSeq Adapter, Index 19 (97% over 39bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA346020.2964404020459031Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG341860.29287646911569404Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG341760.2927907976510255No Hit
ACCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC327160.28028276380942624Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGACTGGCCGTGGTCGAGGTGAGGGGGGGTTCTCTCTCCCTAGATCGGA322370.2761791006518056No Hit
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT308000.2638681111789439Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC306600.26266871067358505No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT297310.25470983160588245No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTAGGGGG267370.2290597950841371TruSeq Adapter, Index 19 (97% over 43bp)
TGAAAATCTTTACTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC261060.2236539256635555Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA248570.2129535597264613Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCTGCTGAACTACTTTGAGAACGAGTAGATCGGAAGAGCACACGTCTG240760.20626261833585238Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAAT235720.20194477651656056TruSeq Adapter, Index 19 (97% over 41bp)
GGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCG228960.19615338550497075No Hit
TGAAAATCTTTGCTCTGAAGTGGATAGATCGGAAGAGCACACGTCTGAAC218320.1870379416642436Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACCCCTCATCCGCTACTTTGACCTCATTAGATCGGAAGAGCACACGTCT218130.18687516588137346Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGACTGGCCGTGGTCGAGGTGAGGGGGGGTTCTCTCTCCCTTTCCGAGG216760.18570146681541516No Hit
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA215230.1843906934059873Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGTGGTGGACTGCTTGCAGTGCTAGATCGGAAGAGCACACGTCTGAACTC210400.18025276166249932Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGACTGGCCGTGGTCGAGGTGAGGGGGGGTTCTCTCTCCCTTTAGATCG209700.1796530614098199No Hit
CGGACTGGCCGTGGTCGAGGTAGATCGGAAGAGCACACGTCTGAACTCCA203680.1744956392367769Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGACTGGCCGTGGTCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAG201570.17268797133227182Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GATACGATCCACTGAGATTCATCCTGTGTTTTTCGGTCTGTAGATCGGAA197620.16930394847786653No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCGG188300.1613193679707634No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCT184430.15800388228809292Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAGAGCACACGTCTGAACT179950.15416580067094462No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAATCTAGATCGGAA175300.15018207756385993No Hit
TGAAAATCTTTGCTCTGAAGTGGAGATCGGAAGAGCACACGTCTGAACTC172880.1481088281188825Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGAGC160640.13762264084345954No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA159910.1369972391513796Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCA157470.1349068554134685Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTGCGTGTTTTCACTGCGGCGACTGTCGCAGATCGGAAGAGCACACGTC154430.13230244288754645No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA147730.12656245475475772Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG146310.12534591995646518Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTTTCCGAGGTCGCGGTCGGACCGGGAAAGATCGGAAGAGCACACGTCTG144330.12364962495602912Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCAAGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC141600.12131079397057938Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGATCCGGCCCGGACTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT140410.12029130354102438Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGAATGTTTACTCGTGAAGGAAAAAAAAAAGATCGGAAGAGCACACGTC139340.11937461886907155No Hit
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTAAAC138390.11856073995472091Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
CGTGGTCGAGGTGAGGGGGGGTTCTCTCTCCCTAGATCGGAAGAGCACAC134190.11496253843864442No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTGGGGGGGGGGT134030.11482546409517483TruSeq Adapter, Index 19 (97% over 39bp)
CCTGTGAATAGGCGGTTGCTCACAGCCCGACCAAGATAGTCGAACTCGAC132380.11341188492814476No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT131990.11307776621593767No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCAGATCGGAAGAGCACA125950.10790320974996097No Hit
TTCCGAGGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTG122100.10460485836022418TruSeq Adapter, Index 19 (100% over 36bp)
AACTTTTGACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTG121990.10451061974908885TruSeq Adapter, Index 19 (100% over 36bp)
CCGGGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTG117890.10099808969768083Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)