FastQCFastQC Report
Wed 30 Apr 2025
1C8_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C8_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10703093
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA246059722.989588149892747Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC4837874.520067236638979No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA2660092.4853469926870675Illumina RNA PCR Primer (100% over 45bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1738011.6238390155070128No Hit
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG1676441.566313588044129Illumina Small RNA Adapter 1 (100% over 26bp)
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG1647041.538844892780059Illumina RNA PCR Primer (100% over 37bp)
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG1247401.1654574990612527Illumina RNA PCR Primer (100% over 36bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1205571.1263753384185302Illumina RNA PCR Primer (100% over 50bp)
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG1042260.9737932763921606Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT782250.730863498990432Illumina DpnII expression Sequencing Primer (100% over 22bp)
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA778770.7276121024081543Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG684330.6393759261925501Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT669190.6252304824409168No Hit
GAGCTAGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC559120.5223910508859448Illumina RNA PCR Primer (100% over 32bp)
GAGGTCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG548920.5128610953861655Illumina RNA PCR Primer (100% over 37bp)
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC499700.4668743885529164Illumina DpnII expression Sequencing Primer (100% over 26bp)
GAGGTCTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG472030.4410220484863581Illumina RNA PCR Primer (100% over 36bp)
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAG398480.3723035948580471No Hit
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC380530.3555327417971609No Hit
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG352270.3291291592065957Illumina DpnII expression Sequencing Primer (96% over 29bp)
ATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCT332370.3105364028883987Illumina DpnII expression Sequencing Primer (100% over 22bp)
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC316550.29575562877011347Illumina DpnII expression Sequencing Primer (100% over 26bp)
CAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA305970.28587063571249915Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTA281360.26287728229587465No Hit
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGAC281240.2627651651723478No Hit
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG273480.2555149245176137Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACT260070.24298583596349205No Hit
CCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGT250330.23388566277056547Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTCGTTCTCAAAGTAGTTCAGCAGAAGATCGTCGGACTGTAGAACTCTG247870.23158726173826577Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAGATCGTCG245680.22954112423390136No Hit
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC240510.22471074482862102No Hit
ACCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG235580.22010459967039434Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT223990.20927595415642936No Hit
TTTTTTTTTCCTTCACGAGTAAACATTCTAGATCGTCGGACTGTAGAACT219330.204922072526138No Hit
CCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAACG214710.20060556327035559Illumina Small RNA Adapter 1 (100% over 26bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT212270.1983258484253103No Hit
GACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC195370.18253602019528373Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGCGTGAGCCTAGAGATCCACCGTCAAAAGTTGATCGTCGGACTGTAGAA179490.16769918751523508No Hit
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATGATCGTCGGACTG179340.16755904111082656No Hit
GCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGT174260.16281274954819136Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT173590.16218676227516662Illumina RNA PCR Primer (100% over 31bp)
AATGAGGTCAAAGTAGCGGATGAGGGGTGGATCGTCGGACTGTAGAACTC172980.1616168335639053Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACTCGTTCTCAAAGTAGTTCAGCAGAAAGATCGTCGGACTGTAGAACTCT165670.15478703212239675Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCCAATCCACGGCCGATTCCTTCCCGGGATCGTCGGACTGTAGAACTCT161340.15074147258180418Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCAGAAATACTCACCACTATACTACAGCGGAGATCGTCGGACTGTAGAAC159900.14939606709948236No Hit
GCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGT146290.136680116672816Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGT143310.13389587477190004Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA137250.12823396003379584No Hit
CGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACGT129900.12136678621777834Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT128640.12018955642074677Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTCCACTTCAGAGCAAGGATTTTCAGATCGTCGGACTGTAGAACTCTGAA125520.11727451120904957Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA124730.11653640681249804Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCT124660.11647100515710739Illumina DpnII expression Sequencing Primer (100% over 22bp)
TTTCCTCCAATCCTCACGGCATAAACGAGATCGTCGGACTGTAGAACTCT123010.11492939470861367Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACAGACCGAAAAACACAGGATGAATCTCAGTGGATCGTATCGATCGTCGG119530.11167799812633601No Hit
CAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT119360.11151916553467302Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTTTCCTCCAATCCTCACGGCATAAACGAGATCGTCGGACTGTAGAACTC118440.11065960092096744Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAACTCTGAAC117350.10964120371559885Illumina DpnII expression Sequencing Primer (100% over 26bp)
TGTGAGATAACCCTCAGCCGTCCGGACCTCGATCGTCGGACTGTAGAACT116190.10855740485483961No Hit
TTCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCT115890.1082771120460226Illumina DpnII expression Sequencing Primer (100% over 22bp)
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA115520.10793141758181489Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG113190.10575447676666923Illumina Small RNA Adapter 1 (100% over 26bp)
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG108990.10183037744323066No Hit
CGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTC108450.10132585038735999Illumina DpnII expression Sequencing Primer (100% over 21bp)
CTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA107770.10069052002070802Illumina DpnII expression Sequencing Primer (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)