FastQCFastQC Report
Wed 30 Apr 2025
1C8_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C8_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10703093
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC242363022.644202007774762Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG4789184.474575713767973No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1759761.6441602441462482No Hit
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC1670641.5608945937403327Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAT1639251.5315666228444431TruSeq Adapter, Index 11 (100% over 41bp)
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACA1250271.168138966932269TruSeq Adapter, Index 11 (100% over 40bp)
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA1024830.9575082641998905Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG780720.7294340056654651Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT769950.7193714938289334Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA676590.6321443717250705Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG659890.6165414053675886No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTAGGGTG634280.5926137425882406TruSeq Adapter, Index 11 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTAGGGGG584210.5458328727966766TruSeq Adapter, Index 11 (97% over 45bp)
CAGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAT558980.5222602475751635TruSeq Adapter, Index 11 (100% over 41bp)
GGGGGACTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC552220.515944316283153TruSeq Adapter, Index 11 (100% over 36bp)
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT491430.4591476501231933Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCAGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACA479770.44825360295383776TruSeq Adapter, Index 11 (100% over 40bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCAGATCGGAAGAGCACA404810.37821777312408666No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT377850.353028792705062No Hit
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA351470.3283817117164169Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTG330210.30851829466491604Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT314270.29362540342310395Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA296790.27729367576269776Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCAGATCGGAAGAGCAC286800.26795992522909035No Hit
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGA276440.2582804802312752No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA269490.2517870301603471Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTAGGATG266340.24884395566776818TruSeq Adapter, Index 11 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTAGTGTG261450.24427518288405045TruSeq Adapter, Index 11 (97% over 46bp)
CGACCAAGATAGTCGAACTCGACCCTGTCAAGATCGGAAGAGCACACGTC260230.2431353254615278No Hit
TTCTGCTGAACTACTTTGAGAACGAGTAGATCGGAAGAGCACACGTCTGA246520.23032594409858909Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGACTGGCCGTGGTCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAG244840.22875630436921365Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGA242980.227018488954548No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCGG234460.21905817318414408No Hit
CGGACTGGCCGTGGTCGAGGTAGATCGGAAGAGCACACGTCTGAACTCCA231330.21613378487881962Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC224960.21018223423827112No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAAAGATCGGAAGAGCACACGTC221390.20684674981334833No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTGGGGGG218560.20420265431684093TruSeq Adapter, Index 11 (97% over 45bp)
TGAAAATCTTTGCTCTGAAGTGGAGATCGGAAGAGCACACGTCTGAACTC210740.1968963551003434Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCG208850.19513051040479606No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCT198980.18590887699471545Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTTTTGACGGTGGATCTCTAGGCTCACGCAAGATCGGAAGAGCACACG178650.16691436765054737No Hit
ATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGAGC177900.16621363562850477No Hit
CTCGGGCTGAGACTTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG175140.16363494178738802Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CACCCCTCATCCGCTACTTTGACCTCATTAGATCGGAAGAGCACACGTCT174660.16318647329328073Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGGATCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG173370.16198121421536746TruSeq Adapter, Index 11 (100% over 35bp)
TTTCTGCTGAACTACTTTGAGAACGAGTAGATCGGAAGAGCACACGTCTG164210.1534229404528205Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTAGTATG163370.15263812058813278TruSeq Adapter, Index 11 (97% over 49bp)
CCGGGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTG162150.15149826316561016Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGGATCCGGCCCGGACTGGCAGATCGGAAGAGCACACGTCTGAACTCC144790.13527865262873076Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC143050.13365295433759194Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG136240.12729030757744514No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAAAAAAAAGATCGGAAGAGCAC136050.1271127887985277No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTAGGAGG131050.12244124198491034TruSeq Adapter, Index 11 (97% over 45bp)
TCCGCTGTAGTATAGTGGTGAGTATTTCTGCAGATCGGAAGAGCACACGT128750.12029233045064637No Hit
TCTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCC128340.11990926361192976Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC126570.11825553603990921Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAAAATCCTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC124470.11629348637818993Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTG123460.11534983392183923Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTTTATGCCGTGAGGATTGGAGGAAAAGATCGGAAGAGCACACGTCTG121440.11346252900913782Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAAAATCTTTGCTCTGAAGTGGGAAGATCGGAAGAGCACACGTCTGAAC120680.11275245389346798Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGAATGTTTACTCGTGAAGGAAAAAAAAAAAAAAAAGATCGGAAGAGCA119380.11153785172192748No Hit
GATACGATCCACTGAGATTCATCCTGTGTTTTTCGGTCTGTAGATCGGAA118170.11040733739303209No Hit
TCGTTTATGCCGTGAGGATTGGAGGAAAAAGATCGGAAGAGCACACGTCT116850.10917404903423712Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC116500.1088470407572839Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTGGGGTG116370.10872558054012985TruSeq Adapter, Index 11 (97% over 45bp)
TATTAATGCTGTAGGTGAAAGACAAGATCGGAAGAGCACACGTCTGAACT115540.10795010376906937Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTTCCGAGGTCGCGGTCGGACCGGGAAAGATCGGAAGAGCACACGTCTG115050.10749229218133488Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGTCCGGACGGCTGAGGGTTATCTCACAAGATCGGAAGAGCACACGTC115020.10746426290045316No Hit
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC111880.10453053150150148Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGGATCCGGCCCGGACTGGCTAGATCGGAAGAGCACACGTCTGAACTC111710.1043716989098385Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT107230.10018599296483736Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)