FastQCFastQC Report
Wed 30 Apr 2025
1C7_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C7_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11471279
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA181214415.797227144418683Illumina DpnII expression Sequencing Primer (100% over 25bp)
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG6940116.049987974313937Illumina RNA PCR Primer (100% over 37bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC2329772.0309592330550066No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1693041.475894710607248No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1525401.3297558188585599Illumina RNA PCR Primer (100% over 45bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1385881.2081303226954903Illumina RNA PCR Primer (100% over 50bp)
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG1273381.1100593055055152Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT1220361.0638395247818486Illumina DpnII expression Sequencing Primer (100% over 22bp)
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG1170861.0206882772182597Illumina RNA PCR Primer (100% over 36bp)
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG1133920.9884861138849468Illumina DpnII expression Sequencing Primer (96% over 29bp)
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA717800.625736676790792Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG569140.49614345532002146Illumina Small RNA Adapter 1 (100% over 26bp)
GGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTA523230.45612176288276135No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT469670.40943124127658304No Hit
AAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG391910.3416445541948723Illumina RNA PCR Primer (100% over 37bp)
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC369720.32230059089313406Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC353250.30794299397652175Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACTCGTTCTCAAAGTAGTTCAGCAGAAGATCGTCGGACTGTAGAACTCTG341780.2979441089350194Illumina DpnII expression Sequencing Primer (100% over 23bp)
GAGCTAGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC328710.2865504360934818Illumina RNA PCR Primer (100% over 32bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG326800.2848854081571898Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG312400.27233231795687296Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG311760.2717744028368589Illumina Small RNA Adapter 1 (100% over 26bp)
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG311180.27126879225934614No Hit
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAG305540.26635216526422206No Hit
CAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA284720.248202489016264Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGT279470.24362584154739853Illumina DpnII expression Sequencing Primer (96% over 28bp)
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC259340.22607766753820563No Hit
ATCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA239290.20859923291901453Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCCAGTCCGGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTC238980.20832899278275774No Hit
AGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT230000.20050074625506012Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGT226790.1977024532312395Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGAC212040.18484425319966502No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA202420.17645809155195336No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT201050.17526380449817322No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC199960.17431360530939924No Hit
ACAGACCGAAAAACACAGGATGAATCTCAGTGGATCGTATCGATCGTCGG193550.16872573668550822No Hit
TTCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCT192880.16814166929424348Illumina DpnII expression Sequencing Primer (100% over 22bp)
CCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAACG190850.1663720322729488Illumina Small RNA Adapter 1 (100% over 26bp)
ATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCT190680.16622383606919508Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG189540.16523004976167Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT189070.16482033084540965No Hit
GCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGT173760.15147395508382283Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAACTC172950.150767843760055Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCCAATCCACGGCCGATTCCTTCCCGGGATCGTCGGACTGTAGAACTCT163120.14219861621358876Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC162710.1418412018398297No Hit
TAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG161230.14055102312479714Illumina RNA PCR Primer (100% over 37bp)
ACCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG158190.13790092630473028Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAAAATGGACAGGCTTCTTGTAGACAAGATCGTCGGACTGTAGAACTCT151670.13221716601958683Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCCTCGACCACGGCCAGTCCGATCGTCGGACTGTAGAACTCTGAACGTGT141800.12361306877811967Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG139650.12173882267182239Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTC137880.12019583866803343Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGATTAAGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGG137380.11975996748052245No Hit
ACGGGATCGGTCACGGTAAGATCGTCGGACTGTAGAACTCTGAACGTGTA134150.11694423960920138Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG130750.11398031553412659Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGTGT129450.11284705044659797Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTTTTTTTTCCTTCACGAGTAAACATTCTAGATCGTCGGACTGTAGAACT127160.1108507604077976No Hit
CCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGT126780.11051949830528925Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT124090.10817451131648005Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCTCTATTCTTGTTCGCCTAACCGGATCGTCGGACTGTAGAACTCTGAA123390.10756429165396467Illumina DpnII expression Sequencing Primer (100% over 25bp)
CAGTTAAGAGCACACTCGGTCTCGATCGTCGGACTGTAGAACTCTGAACG123030.10725046439895673Illumina Small RNA Adapter 1 (100% over 26bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAGATCGTCG114800.10007602465252566No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)