FastQCFastQC Report
Wed 30 Apr 2025
1C7_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C7_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11471279
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC178266115.540211339990945Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAT6909576.023364962180764TruSeq Adapter, Index 3 (100% over 41bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG2310442.014108452945831No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1720621.4999373653103547No Hit
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA1248741.088579573384973Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG1221111.064493331563115Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA1173281.0227978937658129TruSeq Adapter, Index 3 (100% over 40bp)
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA1128450.9837176830935765Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGGGGG937740.817467694753131TruSeq Adapter, Index 3 (97% over 45bp)
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT709760.6187278680956152Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTGGTGGACTGCTTGCAGTGCTAGATCGGAAGAGCACACGTCTGAACTC563000.49079095713738635Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCAGATCGGAAGAGCAC535570.46687906379053284No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG462950.4035731325164352No Hit
CGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAT371430.3237912703544217TruSeq Adapter, Index 3 (100% over 41bp)
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT360610.31435901785668363Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT350930.3059205516664707Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCTGCTGAACTACTTTGAGAACGAGTAGATCGGAAGAGCACACGTCTGA338580.29515453333494895Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGACTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA324280.28268861737213435TruSeq Adapter, Index 3 (100% over 36bp)
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA323340.2818691795396137Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCAGATCGGAAGAGCACA311470.2715215975481025No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGGGGGGGGGG310130.27035346276557304TruSeq Adapter, Index 3 (97% over 41bp)
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC309700.2699786135443136Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA308530.2689586749655378Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTAGGGGG288970.25190739411010754TruSeq Adapter, Index 3 (97% over 45bp)
ACCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC279300.24347764534364477Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA276240.24081011367607744Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT258210.22509259865443076No Hit
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC251230.21900783687677722No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCGGACTGGCAGATCGG240440.20960173665028983No Hit
TGAAAATCTTTGCTCTGAAGTGGATAGATCGGAAGAGCACACGTCTGAAC238880.20824181854525553Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGGGCTGAGACTTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG226270.19724914719622808Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC225250.19635996997370564Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGA209240.18240337454960343No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC203070.17702472409571768No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG200860.17509817344691903No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT198070.17266601222060765No Hit
GATACGATCCACTGAGATTCATCCTGTGTTTTTCGGTCTGTAGATCGGAA191700.16711301329171752No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAAAGATCGGAAGAGCACACGTCTG191110.16659868529045455Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTG189200.16493365735416252Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGCTGTGACCCTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC186710.16276301884035774Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCG186610.16267584460285553No Hit
TGAAAATCTTTGCTCTGAAGTGGAGATCGGAAGAGCACACGTCTGAACTC186340.1624404741615996Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTGGATCCGGCCCGGACTGGCAGATCGGAAGAGCACACGTCTGAACTCC171760.14973047033377881Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAAGATCGGAAGAGCACACGTCT170920.14899820673876035Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGGGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTG165140.14395953581113316Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCGG160380.13981004210602846No Hit
CGGACTGGCCGTGGTCGAGGTAGATCGGAAGAGCACACGTCTGAACTCCA152920.13330684398836434Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGACCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAT152530.13296686446210576TruSeq Adapter, Index 3 (100% over 41bp)
TTGTCTACAAGAAGCCTGTCCATTTTGTAGATCGGAAGAGCACACGTCTG149190.13005524492953227Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAG140700.12265415216559548Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGGGCTGAGACTTGAAGCAAGATCGGAAGAGCACACGTCTGAACTCCA139900.12195675826557788Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAATCTAGATCGGAA136440.11894052964800175No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT135700.11829544029048548Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTACCGTGACCGATCCCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT132440.11545356014791378Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA130400.11367520570286889Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG128710.1122019610890817Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGAATGTTTACTCGTGAAGGAAAAAAAAAAGATCGGAAGAGCACACGTC128190.11174865505407026No Hit
CTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCC124920.1088980574877483Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTTAGGCGAACAAGAATAGAGGCAGATCGGAAGAGCACACGTCTGAAC123180.10738122575521003Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGGGTG121950.10630898263393297TruSeq Adapter, Index 3 (97% over 45bp)
AGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC120630.10515828269890393Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGACCGAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTC120190.10477471605389425Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)