FastQCFastQC Report
Wed 30 Apr 2025
1C3_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C3_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19105567
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA431606322.59060408937353Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC6561633.4344073640944552No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT2580981.3509046865764307Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC2057251.0767803959966225No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT1917351.0035556652152746No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1370000.7170684858502236Illumina RNA PCR Primer (100% over 45bp)
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA1352190.7077465955341707Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1168390.6115442687463817Illumina RNA PCR Primer (100% over 50bp)
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC1048930.5490179904108577Illumina DpnII expression Sequencing Primer (100% over 26bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC997010.521842665020096Illumina DpnII expression Sequencing Primer (100% over 21bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGGCTGTGAGCAACCGCCTATT849290.4445248863852091No Hit
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG776940.40665634262516254Illumina Small RNA Adapter 1 (100% over 26bp)
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC760180.39788403034571024Illumina DpnII expression Sequencing Primer (100% over 26bp)
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG747190.3910849649214807Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGT716950.37525711746738527Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGAC703850.3684004772012262No Hit
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG703830.3683900090481481Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG687370.3597747190648673Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAG669350.3503429131414943No Hit
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC667360.34930133191022283No Hit
GGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTA612400.32053484725158904No Hit
ACCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG562600.29446914608710645Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTCGTTCTCAAAGTAGTTCAGCAGAAGATCGTCGGACTGTAGAACTCTG556030.29103035780094877Illumina DpnII expression Sequencing Primer (100% over 23bp)
CGCGACCTCGGAAAGGGAGAGAGAACCCCCCCTCACCTCGACCACGGCCA544990.28525193730183457No Hit
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT536610.2808657811621084No Hit
AACAGGGTCGAGTTCGACTATCTTGGTCGGGCTGTGAGCAACCGCCTATT534940.2799916903800866No Hit
AGGGAGAGAGAACCCCCCCTCACCTCGACCACGGCCAGTCCGGATCGTCG513320.2686756169026546No Hit
AAAGGGAGAGAGAACCCCCCCTCACCTCGACCACGGCCAGTCCGGATCGT504470.2640434591655929No Hit
CTTCCCGGTCCGACCGCGACCTCGGAAGATCGTCGGACTGTAGAACTCTG495120.25914959760157863Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCCAGTCCGGGCCGGATCCGATCGTCGGACTGTAGAACTCTGAACGTGT489240.25607196059661563Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGATTAAGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGG473260.2477079062872094No Hit
AACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC450690.23589459553856737Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAACTC440480.23055060339219452Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTTTTTTTTCCTTCACGAGTAAACATTCTAGATCGTCGGACTGTAGAACT418930.2192711684505359No Hit
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG415040.21723511267684442Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGGTCCGACCGCGACCTCGGAAGATCGTCGGACTGTAGAACTCTGAACGT409430.21429879573843583Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGGGTCGAGTTCGACTATCTTGGTCGGGCTGTGAGCAACCGCCTATTC408110.21360789763528085No Hit
AGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG403670.21128396765194143Illumina Small RNA Adapter 1 (100% over 26bp)
TTCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCT398690.2086773975354932Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC386410.20224995154553643No Hit
GGCCAATCCACGGCCGATTCCTTCCCGGGATCGTCGGACTGTAGAACTCT384190.20108798655386673Illumina DpnII expression Sequencing Primer (100% over 22bp)
GAGGTCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG383140.2005384085172662Illumina RNA PCR Primer (100% over 37bp)
ATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCT375660.19662331926605475Illumina DpnII expression Sequencing Primer (100% over 22bp)
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG357050.18688270282687763No Hit
GACAGGGTCGAGTTCGACTATCTTGGTCGGGATCGTCGGACTGTAGAACT329970.17270882355912284No Hit
CCACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGT328420.17189754169556967Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAACG320900.167961516138202Illumina Small RNA Adapter 1 (100% over 26bp)
TCCTTCCCGGTCCGACCGCGACCTCGGAAGATCGTCGGACTGTAGAACTC317230.16604061004836967Illumina DpnII expression Sequencing Primer (100% over 21bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATGATCGTCGGACTG316220.16551196831792533No Hit
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG304570.15941426914992893Illumina RNA PCR Primer (100% over 37bp)
TTCCACTTCAGAGCAAGGATTTTCAGATCGTCGGACTGTAGAACTCTGAA292690.15319618622153428Illumina DpnII expression Sequencing Primer (100% over 25bp)
CGCGACCTCGGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC286730.15007667660425886Illumina RNA PCR Primer (100% over 32bp)
ACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTCT282990.14811913197865315Illumina DpnII expression Sequencing Primer (100% over 22bp)
ATCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA280490.14681061284388996Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACAGACCGAAAAACACAGGATGAATCTCAGTGGATCGTATCGATCGTCGG273510.14315722741963113No Hit
GCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGT269230.14091704266091656Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACT258780.13544743267760648No Hit
ACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG256240.13411797723668709Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTC252520.13217090076415947Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACTCATCCTAAGTAGACTTTTCACATTGAAGATCGTCGGACTGTAGAACT250370.13104557430826314No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG236350.12370739900051121No Hit
TCCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA225580.11807029856795143Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA224570.1175416568375071No Hit
GCCAGTCCGGGCCGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT223750.11711246256130478Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGT222520.1164686711470013Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAGATCGTCG219780.11503453417530084No Hit
CTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA215370.1127263064215786Illumina DpnII expression Sequencing Primer (100% over 25bp)
CAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA212920.11144395766951067Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATCATAGATCAGACAGTTCACTAGCAAAGTTGCCACGATCGTCGGACTG212070.11099906116369118No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC209800.10981092578932622No Hit
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG199130.10422616612215695Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCCAGTCCGGGCCGGATCCGATCGTCGGACTGTAGAACTCTGAACGTGTA199100.10421046389253981Illumina DpnII expression Sequencing Primer (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)