FastQCFastQC Report
Wed 30 Apr 2025
1C3_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C3_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19105567
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC400659320.970814422832884Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG6439023.3702323516491295No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG2438881.2765284589564916Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1966411.0292340447158674No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG1887110.9877278177611793No Hit
CCTGTGAATAGGCGGTTGCTCACAGCCCGACCAAGATAGTCGAACTCGAC1447040.7573918115070859No Hit
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT1237510.647722205784314Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTGGGGGG1036170.5423393087470265TruSeq Adapter, Index 19 (97% over 43bp)
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT989590.5179589802281188Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCT960560.5027644560352488Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC729610.38188345836582605Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT696150.3643702382661556Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGACTGGCCGTGGTCGAGGTGAGGGGGGGTTCTCTCTCCCTTTCCGAGG691150.36175319999662925No Hit
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA685420.358754074139752Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGA660720.3458259050882918No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA655080.3428738859202661Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGACTGGCCGTGGTCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAG650680.34057089224308285Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA646680.3384772616274618Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCAGATCGGAAGAGCACA645830.3380323651216423No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT630750.3301393777007508No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCAGATCGGAAGAGCAC595480.31167878974751184No Hit
CGGACTGGCCGTGGTCGAGGTAGATCGGAAGAGCACACGTCTGAACTCCA522320.27338628578780205Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTGCTGAACTACTTTGAGAACGAGTAGATCGGAAGAGCACACGTCTGA521960.27319785903239613Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCG519390.27185270136185957No Hit
CGGACTGGCCGTGGTCGAGGTGAGGGGGGGTTCTCTCTCCCTAGATCGGA508430.2661161534750578No Hit
CGGACTGGCCGTGGTCGAGGTGAGGGGGGGTTCTCTCTCCCTTTAGATCG499560.2614735275849181No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAATCTAGATCGGAA461610.24161020711921294No Hit
TTCCGAGGTCGCGGTCGGACCGGGAAGAGATCGGAAGAGCACACGTCTGA459940.24073611633719114Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG451080.23609872452359043Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAGAGCACACGTCTGAACT436380.2284046320111829No Hit
CGACCAAGATAGTCGAACTCGACCCTGTTAGATCGGAAGAGCACACGTCT428840.2244581383007372Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAAGATCGGAAGAGCACACGTCT411090.21516765244391856Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGAATGTTTACTCGTGAAGGAAAAAAAAAAGATCGGAAGAGCACACGTC402970.21091758229420773No Hit
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA386510.20230229231092697Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCGG382680.20029764099646977No Hit
CTGTGAATAGGCGGTTGCTCACAGCCCGACCAAGATAGTCGAACTCGACC381690.19977946741910357No Hit
CGTGGTGGACTGCTTGCAGTGCTAGATCGGAAGAGCACACGTCTGAACTC379060.1984029052893327Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCGAGGTCGCGGTCGGACCGAGATCGGAAGAGCACACGTCTGAACTCC378500.19810979700314574Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTGGGGGGGGGGG373940.19572305810133772TruSeq Adapter, Index 19 (97% over 39bp)
CTTTCCGAGGTCGCGGTCGGACCGGGAAAGATCGGAAGAGCACACGTCTG373070.1952676934424401Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTG364350.19070357870038612Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAAT361600.18926420765214663TruSeq Adapter, Index 19 (97% over 41bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTG355850.1862546136421913Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTC319080.16700891420809444No Hit
ACCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC307030.16070185197853587Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCGAGGTCGCGGTCGGACCGGGAAGGAAGATCGGAAGAGCACACGTCT297720.1558289267206778Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGAGC296730.15531075314331158No Hit
TGAAAATCTTTGCTCTGAAGTGGAGATCGGAAGAGCACACGTCTGAACTC290540.15207085976563794Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAAT281410.1472921478854828TruSeq Adapter, Index 19 (97% over 41bp)
TGAAAATCCTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC275970.14444481024823813Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAAATCTTTGCTCTGAAGTGGATAGATCGGAAGAGCACACGTCTGAAC271020.14185394236140703Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGACCAAGATAGTCGAACTCGACCCTGTAGATCGGAAGAGCACACGTCTG270950.14181730382563365Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC270390.1415241955394467No Hit
GATACGATCCACTGAGATTCATCCTGTGTTTTTCGGTCTGTAGATCGGAA267760.14014763340967584No Hit
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTAAAC261200.13671407920005724Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
TTCCGAGGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTG260160.13616973523999576TruSeq Adapter, Index 19 (100% over 36bp)
GATGATTGAAATCTTCATGAACGACCCAAAAGGCCAAAAAAAAAAAAAAA251960.13187779247797252No Hit
CTCGGGCTGAGACTTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG249920.13081004086400574Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGAAGAGCACACGTCTGAACT247070.12931832905037574Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTGATGAAGAAATGCTATGTAAGCTTCACCCGCATTCTTGTGAGCTTTA245960.1287373465545409No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCAAGATCGGAAGAGCACACGTC245270.12837619527334623No Hit
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAAGCACACGTCTGAACT240900.1260889038257802No Hit
CAGCTGTGACCCTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC238860.12502115221181345Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCAATGTGAAAAGTCTACTTAGGATGAGTAGATCGGAAGAGCACACGTC235170.12308977796890298No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT234520.12274956299386457Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGA214830.11244366628846973No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG214650.11234945291076678No Hit
AGGCAATGATGTCTTTAATATACGCTGATGTCCAGTGATAAGAAACATGC211920.1109205500156054No Hit
TCCGGCCCGGACTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC209460.10963296718699841Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTGGATCCGGCCCGGACTGGCAGATCGGAAGAGCACACGTCTGAACTCC208080.10891066462460915Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAAAATCTTTGCTCTGAAGTGGGAAGATCGGAAGAGCACACGTCTGAAC206270.1079632967710406Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT203330.10642447826855911No Hit
TTTAAGGTGTTACACGGTTATAACTGGAGAACCTGGTGTAGAAAAAAAAA202950.1062255833600751No Hit
GTGGCAACTTTGCTAGTGAACTGTCTGATCTATGATTAGATCGGAAGAGC202380.1059272409973491No Hit
GAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA192470.10074027114714784Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)