FastQCFastQC Report
Wed 30 Apr 2025
1C2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17964160
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA4446982.475473387010581Illumina DpnII expression Sequencing Primer (100% over 25bp)
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC2820311.569964863372404Illumina DpnII expression Sequencing Primer (100% over 26bp)
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA2813331.5660793491039937Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGAC2544551.416459216573444No Hit
AGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG2389381.3300816737325876Illumina Small RNA Adapter 1 (100% over 26bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG2263541.2600310841141473Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC2177621.2122025187929744No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1374260.7650009797285261No Hit
CCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGT1327200.7388043749332003Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG1309420.7289068901635256Illumina DpnII expression Sequencing Primer (96% over 29bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT1279530.7122682051373401No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT1034460.5758465745128077No Hit
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAG981720.5464881185649649No Hit
ATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCT956520.5324601873953472Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG946780.5270382806655028Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAAGGGAGAGAGAACCCCCCCTCACCTCGACCACGGCCAGTCCGGATCGT938310.5223233371334924No Hit
CGCGACCTCGGAAAGGGAGAGAGAACCCCCCCTCACCTCGACCACGGCCA911860.5075995760447469No Hit
ACCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG898060.49991761373757526Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGGAGAGAGAACCCCCCCTCACCTCGACCACGGCCAGTCCGGATCGTCG862380.4800558445259895No Hit
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG826560.4601161423634615Illumina Small RNA Adapter 1 (100% over 26bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT815190.4537868734190744Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCCAATCCACGGCCGATTCCTTCCCGGGATCGTCGGACTGTAGAACTCT772800.43018988920161033Illumina DpnII expression Sequencing Primer (100% over 22bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATGATCGTCGGACTG729810.4062589066229649No Hit
GACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC716390.3987884766112081Illumina DpnII expression Sequencing Primer (100% over 21bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGGCTGTGAGCAACCGCCTATT697800.38844009405393853No Hit
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTGATCGTCGG604320.3364031493818804No Hit
GGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTA569360.3169421782037123No Hit
AGAACACCGCCCCAACGACCGGCAGCAGTCGCGGGATCGTCGGACTGTAG566390.3152888863158645No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA566180.31517198688945103Illumina RNA PCR Primer (100% over 45bp)
GGCCAGTCCGGGCCGGATCCGATCGTCGGACTGTAGAACTCTGAACGTGT562500.31312346360753857Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCGATCGTCGGA501550.27919479675086395No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT456000.2538387544978446Illumina RNA PCR Primer (100% over 50bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCCGGGAT443460.24685818874915386No Hit
GCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGT431600.2402561544764687Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACAGGGTCGAGTTCGACTATCTTGGTCGGGCTGTGAGCAACCGCCTATT419260.23338692151483845No Hit
ACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG413860.23038093626420605Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCT390240.21723253411236593Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGGGAGAGAGAACCCCCCCTCACCTCGACCACGGATCGTCGGACTGTAGA388570.21630290534041113No Hit
CGGTCCGACCGCGACCTCGGAAGATCGTCGGACTGTAGAACTCTGAACGT370580.20628852114432292Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCGACCTCGGAAAGGGAGAGAGAACCCCCCCTCGCCTCGACCACGGCCA369210.20552589155295878No Hit
CGCGACCTCGGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC345450.19229955645017635Illumina RNA PCR Primer (100% over 32bp)
GACCGCGACCTCGGAAAGGGAGATCGTCGGACTGTAGAACTCTGAACGTG342050.19040689907014854Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGACT329610.18348199971498805No Hit
ACAGGGTCGAGTTCGACTATCTTGGTCGGGCTGTGAGCAACCGCCTATTC325190.18102154512095195No Hit
GACAGGGTCGAGTTCGACTATCTTGGTCGGGATCGTCGGACTGTAGAACT319780.17800999323096656No Hit
CTTCCCGGTCCGACCGCGACCTCGGAAGATCGTCGGACTGTAGAACTCTG311910.17362904806013751Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTA308960.17198688945099577No Hit
AACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC308740.17186442338522925Illumina DpnII expression Sequencing Primer (100% over 21bp)
CAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA306790.17077892871138978Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGGGATCGGTCACGGTAAGATCGTCGGACTGTAGAACTCTGAACGTGTA263080.14644714809932666Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCGCGGTCCGTTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT262140.1459238840001425Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATCATAGATCAGACAGTTCACTAGCAAAGTTGCCACGATCGTCGGACTG258570.14393659375111334No Hit
AGTGTCGGCGCCAGTCAGGGATCGTCGGACTGTAGAACTCTGAACGTGTA257600.1433966297338701Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG254890.1418880704692009Illumina DpnII expression Sequencing Primer (100% over 23bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCCGGTCC254360.1415930385834907No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACAGATCG253360.14103637464818838No Hit
AGATTAAGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGG243200.13538066906551713No Hit
GACCGCGACCTCGGAAAGGGAGAGAGAACCCCCCCTCACCTCGACCACGG238060.13251941643806334No Hit
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCGATCGTCGGACTGT235240.1309496241405109No Hit
ACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACG231670.1289623338914817Illumina Small RNA Adapter 1 (100% over 26bp)
GACCGCGGTCCGTTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA229210.12759294061063808Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG226100.12586171577184793Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG223300.12430305675300155Illumina RNA PCR Primer (100% over 37bp)
CCTCGACCACGGCCAGTCCGATCGTCGGACTGTAGAACTCTGAACGTGTA219000.12190940183120168Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGGAGAGAGAACCCCCCCTCACCTCGACCACGATCGTCGGACTGTAGAA216440.12048434215682782No Hit
CGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC216090.120289509779472Illumina RNA PCR Primer (100% over 30bp)
GCCAGTCCGGGCCGGATCCGATCGTCGGACTGTAGAACTCTGAACGTGTA215250.1198219120738181Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTGATCGTCGGACT210720.11730022444689872No Hit
GCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGT208690.11617019665823507Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACG208370.11599206419893833Illumina Small RNA Adapter 1 (100% over 26bp)
TCCTTCCCGGTCCGACCGCGACCTCGGAAGATCGTCGGACTGTAGAACTC207970.1157693986248174Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTCT207210.11534633403398767Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGACAGGGTCGAGTTCGACTATCTTGGTCGGGCTGTGAGCAACCGCCTAT203740.11341471017848873No Hit
CCTCACCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAA201850.11236261534076739Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG196200.10921746410630945Illumina Small RNA Adapter 1 (100% over 26bp)
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCCAGTGATCGTCGGA192860.1073582065623998No Hit
CGCGACCTCGGAAAGGGAGAGAGAACCCCCCCTCAGATCGTCGGACTGTA190810.10621704549503011No Hit
GACCGCGACCTCGGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT187940.10461942000071253Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT187370.10430212155759022Illumina RNA PCR Primer (100% over 31bp)
GGCTCTTGTCCCCCATGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT186580.10386235704870142Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCCAGTAACGTAGATC181070.10079513876518578No Hit
GACCCGAGGGTCACAGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA181010.10076173892906765Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCAATCCACGGCCGATTCCTTCCGATCGTCGGACTGTAGAACTCTGAA180820.10065597278136021Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCCAGTCCGGGCCGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT180730.10060587302718302Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACAGGGTCGAGTTCGACTATCTTGGTCGGGATCGTCGGACTGTAGAACT180690.10058360646977092No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)