FastQCFastQC Report
Wed 30 Apr 2025
1C2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17964160
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC4378002.4370747087534292Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT2814141.5665302468915885Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT2774591.544514188250383Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGA2492371.3874124924293705No Hit
CGTGGTGGACTGCTTGCAGTGCTAGATCGGAAGAGCACACGTCTGAACTC2374271.3216704816701699Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA2243291.2487586394242758Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG2160401.202616765827069No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1395300.7767131889272864No Hit
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA1298290.7227112205636111Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGACTGGCCGTGGTCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAG1297220.7221155901528377Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCTGTGAATAGGCGGTTGCTCACAGCCCGACCAAGATAGTCGAACTCGAC1272780.7085107235740497No Hit
GGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCG1248630.6950672895364993No Hit
CGGACTGGCCGTGGTCGAGGTGAGGGGGGGTTCTCTCTCCCTTTCCGAGG1171290.6520149007802202No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG1020880.5682870782714026No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCAGATCGGAAGAGCACA993400.5529899533292957No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTG953310.5306732961630268Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA934170.520018748441341Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGACTGGCCGTGGTCGAGGTGAGGGGGGGTTCTCTCTCCCTTTAGATCG931120.518320923438669No Hit
CGGACTGGCCGTGGTCGAGGTAGATCGGAAGAGCACACGTCTGAACTCCA881550.4907270921657344Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGACTGGCCGTGGTCGAGGTGAGGGGGGGTTCTCTCTCCCTAGATCGGA857640.4774172574726567No Hit
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC826580.46012727564216754Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG815580.45400397235384227Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTG776950.43250004453311486Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCT728420.40548514375289463Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGAGC719010.40024693612170004No Hit
AGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAA595250.3313542074886886No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCAGATCGGAAGAGCAC578690.32213585272008266No Hit
CCGCGACTGCTGCCGGTCGTTGGGGCGGTGTTCTAGATCGGAAGAGCACA569840.3172093768926574No Hit
GGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG554160.30848088638711746Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGACTGGCCGTGGTCGAGGCGAGGGGGGGTTCTCTCTCCCTTTCCGAGG510600.28423260536534967No Hit
GGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAG491240.27345559157789734No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGA439480.24464266628665077No Hit
CTCGGGCTGAGACTTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG430630.2397161904592255Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGCTGTGACCCTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC408120.2271856852755709Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGTGGTCGAGGTGAGGGGGGGTTCTCTCTCCCTAGATCGGAAGAGCACAC387550.2157351081264028No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAAAGATCGGAAGAGCACACGTCTG386350.21506711140404006Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCGAGGTCGCGGTCGGACCGAGATCGGAAGAGCACACGTCTGAACTCC367690.2046797623712993Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCA344370.19169835940004987Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCCGAGGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC341030.18983910185614022TruSeq Adapter, Index 11 (100% over 36bp)
CCGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTC326040.1814947094659589No Hit
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTAGATCGGAAGAG323890.18029788200505897No Hit
CCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCAC311000.17312248387901244No Hit
CGACCAAGATAGTCGAACTCGACCCTGTTAGATCGGAAGAGCACACGTCT307220.17101829420356976Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCGAGGTCGCGGTCGGACCGGGAAGAGATCGGAAGAGCACACGTCTGA307180.17099602764615768Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA299410.16667074886885888Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGAACGGACCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC262530.1461409829349104Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTACCGTGACCGATCCCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT259650.1445377908012398Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTGGCAACTTTGCTAGTGAACTGTCTGATCTATGATTAGATCGGAAGAGC255940.1424725676012683No Hit
CCTGACTGGCGCCGACACTAGATCGGAAGAGCACACGTCTGAACTCCAGT255530.14224433538779438Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA252540.1405799102212405Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATC251460.13997871317111404No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAATCTAGATCGGAA241750.1345735063593288No Hit
CTGTGAATAGGCGGTTGCTCACAGCCCGACCAAGATAGTCGAACTCGACC240120.13366614414478606No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCA234320.1304374933200328No Hit
CCGAACGGACCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA232000.12914603299013144Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGGGCTGAGACTTGAAGCGGTAGATCGGAAGAGCACACGTCTGAACTC230220.1281551711852934Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC226720.12620684741173538Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAT219980.12245493248779793TruSeq Adapter, Index 11 (100% over 41bp)
GTGGTCGAGGTGAGGGGGGGTTCTCTCTCCCTAGATCGGAAGAGCACACG216080.120283943140119No Hit
GGACTGGCCGTGGTCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGT214630.11947678043393067Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTTTCCGAGGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG214590.11945451387651858Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGATCCGGCCCGGACTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT213940.11909268231857209Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGAG208450.11603659731376252No Hit
CGACCAAGATAGTCGAACTCGACCCTGTAGATCGGAAGAGCACACGTCTG208220.11590856460864299Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGGATCCGGCCCGGACTGGCAGATCGGAAGAGCACACGTCTGAACTCC207470.11549106665716627Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCGAGGTCGCGGTCGGACCGGGAAGGAAGATCGGAAGAGCACACGTCT206400.11489543624639283Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGACTGGCCGTGGTCGAGGTGAAGATCGGAAGAGCACACGTCTGAACTC204770.11398807403185007Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCTGTGAATAGGCGGTTGCTCACAGCCCGACCAAGATAGTCGAACTCGA202890.11294154583348177No Hit
CGTGGTGGACTGCTTGCAGTGCCAGATCGGAAGAGCACACGTCTGAACTC200250.11147195304428373Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGACTGGCCGTGGTCGAGGTGAGGAGATCGGAAGAGCACACGTCTGAAC197770.11009142648473404Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTGGCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAG197250.10980196123837685No Hit
TTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC189620.1055546154120204Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAGGGGGGGTTCTCTCTCCCTTTCCGAGGTCGCGAGATCGGAAGAGCAC189550.10551564893654922No Hit
ATGGGGGACAAGAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC187180.1041963554098828Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTGGATCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG186710.1039347233602907TruSeq Adapter, Index 11 (100% over 35bp)
TACGTTACTGGCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGAT184470.10268779614521359No Hit
TCCGGCCCGGACTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC182370.10151880188107877Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGACCAAGATAGTCGAACTCGACCCTGTTAGATCGGAAGAGCACACGTC181010.10076173892906765No Hit
GGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTGAAC180140.10027744130535465Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)