FastQCFastQC Report
Wed 30 Apr 2025
1C1_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C1_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18542786
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC70

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA8984284.845161886676576Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT7797374.205069292176483Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA6522883.517745391657974Illumina DpnII expression Sequencing Primer (100% over 25bp)
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG5275872.8452412706483265Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC5055662.726483496061487Illumina RNA PCR Primer (100% over 30bp)
CAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT4823322.6011840939112387Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAG1951431.0523930977793736Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG1769540.9543010419254152Illumina Small RNA Adapter 1 (100% over 26bp)
AGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA1596300.8608738729983726Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC1485150.8009314242207185Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA1266150.6828261945103611Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGT1261290.6802052291387066Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG1224320.6602675563423964Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG1082680.5838820552639716Illumina DpnII expression Sequencing Primer (100% over 23bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC1003560.5412131704480653Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC1001900.5403179435927266No Hit
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA746950.4028251202381347Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCT727290.39222261422851995Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAG671900.3623511590976674Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGAAC659740.35579335273566765Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTA648210.34957530114406754No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC622030.33545660290745954No Hit
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG582560.31417069689527777Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG572840.30892876615196874Illumina Small RNA Adapter 1 (100% over 26bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA559710.3018478453022108Illumina RNA PCR Primer (100% over 45bp)
GGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT552190.2977923597888688Illumina RNA PCR Primer (100% over 31bp)
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAG534980.28851112233080833No Hit
CGCGACCTCGGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC531320.28653730890277224Illumina RNA PCR Primer (100% over 32bp)
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG514920.2776928990066541Illumina RNA PCR Primer (100% over 36bp)
AGCTGCTCCCACTTATTCTACACCTCGATCGTCGGACTGTAGAACTCTGA477370.25744243610426176Illumina DpnII expression Sequencing Primer (100% over 24bp)
CAGTTAAGAGCACACTCGGTCTCGATCGTCGGACTGTAGAACTCTGAACG476640.25704875200522725Illumina Small RNA Adapter 1 (100% over 26bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGATCGTCGGACTGTAGAACTCT458040.2470178968791421Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCGAGCTGCTCCCACTTATTCTACACCTCGATCGTCGGACTGTAGAACTC439030.236765931505654Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCCAATCCACGGCCGATTCCTTCCCGGGATCGTCGGACTGTAGAACTCT436140.2352073739081064Illumina DpnII expression Sequencing Primer (100% over 22bp)
CAGTTAAGAGCACACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC422720.22797005800530729Illumina RNA PCR Primer (100% over 30bp)
AGTTAAGAGCACACTCGGTCTCGATCGTCGGACTGTAGAACTCTGAACGT420000.22650318026643892Illumina DpnII expression Sequencing Primer (96% over 28bp)
ATTACTTCGGTTCCAGAAACCAACAAAATAGAACGATCGTCGGACTGTAG375840.2026879887412819No Hit
TGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG374090.20174422549017176Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT370910.200029272839583Illumina RNA PCR Primer (100% over 50bp)
GGTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTAGAACTCTGA370650.19988905658513234Illumina DpnII expression Sequencing Primer (100% over 24bp)
GGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT364050.19632972089523118Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG355620.19178347849131194Illumina RNA PCR Primer (100% over 37bp)
GACCGCGACCTCGGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT351690.1896640558759617Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG341890.18437898166974476Illumina DpnII expression Sequencing Primer (96% over 29bp)
GTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT336320.1813751180647827Illumina RNA PCR Primer (100% over 31bp)
CGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACGT324620.17506538661450335Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCCAATCCACGGCCGATTCCTTCCGATCGTCGGACTGTAGAACTCTGAA321200.1732210035751909Illumina DpnII expression Sequencing Primer (100% over 25bp)
TAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA320790.17299989332778795Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATCCACGGCCGATTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT312080.1683026488036911Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATCCAACCGCTTCAAGTCTGATCGTCGGACTGTAGAACTCTGAACGTGTA310130.1672510268953112Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC291570.15724174350068Illumina RNA PCR Primer (100% over 30bp)
CCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGT285350.1538873392595913Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGGTCCGACCGCGACCTCGGAAGATCGTCGGACTGTAGAACTCTGAACGT282540.15237192512495154Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACT281620.15187577530151078No Hit
GGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC274770.14818161629002244Illumina RNA PCR Primer (100% over 32bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT266940.1439589498579124Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACG263670.14219546081155227Illumina Small RNA Adapter 1 (100% over 26bp)
ACCTACCTTCCACGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT252710.13628480639317092Illumina RNA PCR Primer (100% over 31bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT242240.1306384056851004No Hit
GACAGGGTCGAGTTCGACTATCTTGGTCGGGATCGTCGGACTGTAGAACT239590.12920927847627645No Hit
TCCAACCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGTA238630.1286915569213817Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCAGTTAAGAGCACACGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT237610.12814147776930607Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC237230.1279365463204936Illumina RNA PCR Primer (100% over 30bp)
ATCCAACCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGT235150.12681481628488836Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCGGGCTGTAACAGTCCGGAGCCGAGACTCCAGGATCGTCGGACTGTA229620.12383252441138025No Hit
GGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT228600.12328244525930461Illumina RNA PCR Primer (100% over 35bp)
GGTTCCAGAAACCAACAAAATAGAACGATCGTCGGACTGTAGAACTCTGA225460.1215890643401698Illumina DpnII expression Sequencing Primer (100% over 24bp)
GTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTAGAACTCTGAA218440.11780322547000217Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG216800.11691878448039038Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCAGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTA215420.11617455974522924Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCAATCCACGGCCGATTCCTGATCGTCGGACTGTAGAACTCTGAACGT214690.11578087564619469Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCCAGTCCGGGCCGGATCGATCGTCGGACTGTAGAACTCTGAACGTGTA207430.1118656063873034Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAAACGTTCGAGTTGGCTATCGGTAGTGGATCGTCGGACTGTAGAACTCT201270.10854355974339563Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGCCTTAACTCCAGAAAGTGGATCGTCGGACTGTAGAACTCTGAACGTGT200470.1081121251143167Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCAACCGCTTCAAGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA197390.1064511017923628Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATGGATCGGAGTGGTTCCACTACGTCATAGACACCAGGAGATCGTCGGA194520.10490333006054214No Hit
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT187080.10089098801010808No Hit
CGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC186160.10039483818666732Illumina RNA PCR Primer (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)