FastQCFastQC Report
Wed 30 Apr 2025
1C1_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C1_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18542786
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC71

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA8750544.7191074739254395Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC7631504.11561671476983Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC6420523.4625433308673252Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA5194882.801563907386948Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT4958802.6742475483457557Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4699632.534478907322772Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1908311.0291387712720192Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC1769870.9544790087099101Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1569140.8462266673411428Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT1479600.7979383464814834Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGGGCTGAGACTTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG1258760.6788408171242444Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGTGTGCTCTTAACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1240290.6688800701253845Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAGCTGTGACCCTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC1205880.6503229881421271Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA1070200.5771516750503404Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCT1020950.5505914806976686Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG993330.5356962001287185No Hit
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT736370.3971193972685658Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTG723080.38995218949299204Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCAGATCGGAAGAGCAC659840.35584728206430255No Hit
GAGTGTGCTCTTAACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC657320.3544882629827039Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGACTGCTGCCGGTCGTTGGGGCGAGATCGGAAGAGCACACGTCTGAACT649940.35050827852945077Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT632440.34107064601834913No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA578770.31212677534001637Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGTGGACTGCTTGCAGTGCTAGATCGGAAGAGCACACGTCTGAACTC567740.30617837039159057Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTGGATCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT550480.29687016826921264TruSeq Adapter, Index 3 (100% over 35bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCAGATCGGAAGAGCACA542300.29245874918688053No Hit
TTCCGAGGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA523270.2821959979476654TruSeq Adapter, Index 3 (100% over 36bp)
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA514720.2775850403493844TruSeq Adapter, Index 3 (100% over 40bp)
GAGGTGTAGAATAAGTGGGAGCAGCTAGATCGGAAGAGCACACGTCTGAA471490.25427139158053164Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGACCGAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTC470350.25365659723409417Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCTG462410.2493746085404858Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTG439130.23681986083428888Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGTGTAGAATAAGTGGGAGCAGCTCGCAGATCGGAAGAGCACACGTCT437740.23607024316626424Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGACCGAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCC414700.223644925848791Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGGGGG412230.2223128714315098TruSeq Adapter, Index 3 (97% over 45bp)
GTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT409390.2207812784982796Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGAGCTGTTCTTCCTCGCCGAGACCAGATCGGAAGAGCACACGTCTGAA379310.20455933644491175Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGGGCTGAGACTTGAAGCAAGATCGGAAGAGCACACGTCTGAACTCCA374320.20186826294603194Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTCTATTTTGTTGGTTTCTGGAACCGAAGTAATAGATCGGAAGAGCACA372110.20067642478320138No Hit
GAGTGTGCTCTTAACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC365440.19707933856325582Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAT352530.19011706223649455TruSeq Adapter, Index 3 (100% over 41bp)
TTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC351280.18944294562855873Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGACTGGCCGTGGTCGAGGTAGATCGGAAGAGCACACGTCTGAACTCCA335500.18093289756997682Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTGTGCTCTTAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT333240.17971409474282882TruSeq Adapter, Index 3 (100% over 35bp)
GGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTGAAC321020.17312393078364816Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCC319630.1723743131156235Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGTGTGCTCTTAACTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA317320.17112854562415808Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAATCGGCCGTGGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC306190.16512621134709748Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTGAACTCCAGT304790.16437120074620934Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAGTGTGCTCTTAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT287870.15524635834118994Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGACCAAGATAGTCGAACTCGACCCTGTCAAGATCGGAAGAGCACACGTC282550.15237731805781504No Hit
CTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCC282110.15214002901182164Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCGAGGTCGCGGTCGGACCGAGATCGGAAGAGCACACGTCTGAACTCC279760.15087268978890225Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTGGATCCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA273270.14737267636049944TruSeq Adapter, Index 3 (100% over 36bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG267760.1444011703527183Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGGCTGAGACTTGAAGCGGTAGATCGGAAGAGCACACGTCTGAACTC262760.14170470392097498Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTGGAAGGTAGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT249330.13446199508531242TruSeq Adapter, Index 3 (100% over 35bp)
CCGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTC244880.13206213996106086No Hit
AGTGTGCTCTTAACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT241250.13010450533161522Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG239250.1290259187589179No Hit
GAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT233970.12617845020699694Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTGTGCTCTTAACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC232150.12519693642584237Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTGAACTCCAG230150.12411834985314504Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTGGAGTCTCGGCTCCGGACTGTTACAGCCCGGCTAGATCGGAAGAGCAC228340.12314222900485397No Hit
CGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGC226800.12231171734387702TruSeq Adapter, Index 3 (100% over 39bp)
GTTCTATTTTGTTGGTTTCTGGAACCAGATCGGAAGAGCACACGTCTGAA224300.12096348412800537Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGAGCTGTTCTTCCTCGCCGAGACAGATCGGAAGAGCACACGTCTGAAC222030.1197392883679939Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA215790.11637409826117823Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTGAACTCC215600.11627163253677197Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGAGTGTGCTCTTAACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT211500.11406053006274247Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CACTACCGATAGCCAACTCGAACGTTTCAGATCGGAAGAGCACACGTCTG204800.11044726504420642Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT203300.10963832511468341Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CACTTTCTGGAGTTAAGGCTAGATCGGAAGAGCACACGTCTGAACTCCAG199370.10751890249933317Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTGGTGTCTATGACGTAGTGGAACCACTCCGATCCATCAGATCGGAAG195730.10555587493702402No Hit
GACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA193230.10420764172115236Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)