FastQCFastQC Report
Wed 30 Apr 2025
1B8_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B8_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9848817
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC70

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG6020756.1131707493397425Illumina RNA PCR Primer (100% over 37bp)
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG2371582.4079846340936175Illumina DpnII expression Sequencing Primer (96% over 29bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA2268722.3035456948788875Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG1839451.867686240895734Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG1803191.8308696364243544Illumina Small RNA Adapter 1 (100% over 26bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1773461.800683269879012Illumina RNA PCR Primer (100% over 50bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG1293441.3132947845411282Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA1274841.294409267630823Illumina DpnII expression Sequencing Primer (100% over 25bp)
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC1185931.2041344660988218Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC983700.9988001604659726No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC924820.9390163305907704No Hit
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG914040.9280708535857657Illumina RNA PCR Primer (100% over 36bp)
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAG817180.8297240166001663No Hit
TTCCACTTCAGAGTAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA788940.8010505221083912Illumina DpnII expression Sequencing Primer (100% over 25bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA731990.743226318450226Illumina RNA PCR Primer (100% over 45bp)
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA660420.6705576923604124Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG654620.6646686602055861Illumina Small RNA Adapter 1 (100% over 26bp)
GGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTA641180.6510223512123334No Hit
ATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCT591740.6008234288442967Illumina DpnII expression Sequencing Primer (100% over 22bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT463880.47100073034152223Illumina DpnII expression Sequencing Primer (100% over 22bp)
CAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA405360.4115824266000678Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG357570.36305883234504205Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTCG302800.3074480924967943No Hit
AAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG301620.3062499790583986Illumina RNA PCR Primer (100% over 37bp)
ACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG299390.303985747729905Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGT297060.30161998136425927Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT288580.2930098102137546No Hit
AGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT283920.2882782774824631Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCTCGACCACGGCCAGTCCGATCGTCGGACTGTAGAACTCTGAACGTGT270710.2748654990746604Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT265390.2694638350981646No Hit
GGCCAATCCACGGCCGATTCCTTCCCGGGATCGTCGGACTGTAGAACTCT264130.26818449363004715Illumina DpnII expression Sequencing Primer (100% over 22bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGAC259640.26362557046191437No Hit
GCCAGTCCGGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTC257190.261137962051686No Hit
TGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG244380.24813132379249203Illumina DpnII expression Sequencing Primer (96% over 29bp)
TTCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCT238450.2421102960893679Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC222030.2254382429889803Illumina RNA PCR Primer (100% over 30bp)
ACCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG216510.2198335089381801Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCCAGTCCGGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGT210570.21380232773134072No Hit
AGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG207130.2103095224533058Illumina Small RNA Adapter 1 (100% over 26bp)
GCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGT202290.20539522665514043Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTCGG201000.2040854246758773No Hit
GGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC200460.20353713547525557Illumina RNA PCR Primer (100% over 32bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA199830.20289746474119685No Hit
ACGGGATCGGTCACGGTAAGATCGTCGGACTGTAGAACTCTGAACGTGTA195850.19885637026254016Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGT194980.19797301543931622Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACCCATCCGATCGTCGGA192190.19514018790277046No Hit
CAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT190300.1932211757005943Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT180870.18364642169714393Illumina RNA PCR Primer (100% over 31bp)
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG178640.18138219036865036No Hit
CCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGT171230.173858444115674Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAA170460.17307662432960225Illumina DpnII expression Sequencing Primer (100% over 25bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTGATCGTCGG169180.17177697585405435No Hit
CGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC164690.16721805268592158Illumina RNA PCR Primer (100% over 30bp)
GGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT156160.1585571140168408Illumina RNA PCR Primer (100% over 35bp)
CAGTTAAGAGCACACTCGGTCTCGATCGTCGGACTGTAGAACTCTGAACG154810.1571863910152864Illumina Small RNA Adapter 1 (100% over 26bp)
GGCCAATCCACGGCCGATTCCTTCCGATCGTCGGACTGTAGAACTCTGAA153910.15627257568091682Illumina DpnII expression Sequencing Primer (100% over 25bp)
TAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG151240.15356159018895366Illumina RNA PCR Primer (100% over 37bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCT136900.13900146586133136Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCCAACCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGTA133770.13582341919846821Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATCCAACCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGT131160.13317335472879635Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGCTAGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC128160.13012730361423103Illumina RNA PCR Primer (100% over 32bp)
GACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG124230.12613697665415044Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGATTAAGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGG122110.12398443386652427No Hit
GGCTCTTGTCCCCCATGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT120580.12243094779809595Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTG116470.11825785777114144Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT115850.11762834054079795No Hit
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC115620.11739480995534793No Hit
ACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACG112280.1140035397144652Illumina Small RNA Adapter 1 (100% over 26bp)
TGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAACTCTGAAC111970.11368878109929345Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAG109930.11161746634138901No Hit
CGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACGT108520.11018582231754333Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAA108170.10983044968751068Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCCTCTATTCTTGTTCGCCTAACCGGATCGTCGGACTGTAGAACTCTGAA108150.10981014268008026Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGACT107640.10929231399060416No Hit
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATGATCGTCGGACTG106360.10799266551505629No Hit
ATCCAACCGCTTCAAGTCTGATCGTCGGACTGTAGAACTCTGAACGTGTA103320.10490600038563008Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTA102390.10396172454011482No Hit
CGCGACCTCGGAAAGGGAGAGAGAACCCCCCCTCGCCTCGACCACGGCCA101870.10343374234692349No Hit
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCGATCGTCGGACTGT100180.10171780021905169No Hit
GGCCTCTATTCTTGTTCGCCTAACCGGATCGTCGGACTGTAGAACTCTGA99660.10118981802586036Illumina DpnII expression Sequencing Primer (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)