FastQCFastQC Report
Wed 30 Apr 2025
1B8_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B8_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9848817
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTAT6026376.118877018427695TruSeq Adapter, Index 10 (100% over 41bp)
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA2337652.3735337959878833Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC2241592.2759992392995017Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA1840881.86913819192701Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC1801471.8291232337853367Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA1283131.3028265222107385Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC1258411.2777270610267204Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT1180741.198864797670624Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG961130.9758837025807262No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT939210.9536272224369688No Hit
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA921360.9355032183053051TruSeq Adapter, Index 10 (100% over 40bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCAGATCGGAAGAGCACA834430.8472388105089169No Hit
TGAAAATCTTTACTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC778360.7903081151776908Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCAGATCGGAAGAGCAC655670.665734778095684No Hit
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT653680.6637142308563556Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTGGTGGACTGCTTGCAGTGCTAGATCGGAAGAGCACACGTCTGAACTC650460.6604448026600555Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTG589290.5983358204340683Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG465370.4725136023950897Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA393940.39998712535728914Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA354380.3598198646598876Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTAGGGGG320580.3255010221024515TruSeq Adapter, Index 10 (97% over 45bp)
CTCGGGCTGAGACTTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG297330.30189412596457016Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGCTGTGACCCTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC295880.30042186792586356Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGAGATCGGA295340.29987357872524184No Hit
CGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTAT287870.29228891144997415TruSeq Adapter, Index 10 (100% over 41bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG281340.2856586735239369No Hit
GAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC278230.2825009338685042Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAG270020.27416490731831045Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGGGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTG267970.27208343905669075Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCG260850.2648541444114557No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCGGACTGGCAGATCGG260410.26440739024798615No Hit
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGA254960.2588737307231925No Hit
CTCGGGCTGAGACTTGAAGCAAGATCGGAAGAGCACACGTCTGAACTCCA245520.24928882321602683Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTTCCGAGGTCGCGGTCGGACCGGGAAAGATCGGAAGAGCACACGTCTG236720.24035373994663523Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT218540.2218946701923693Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCGGACTGGCCAGATCG211660.21490905963629947No Hit
CGGACTGGCCGTGGTCGAGGTAGATCGGAAGAGCACACGTCTGAACTCCA210000.2132235780195733Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTGGATCCGGCCCGGACTGGCTAGATCGGAAGAGCACACGTCTGAACTC204760.20790314207279917Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTGGATCCGGCCCGGACTGGCAGATCGGAAGAGCACACGTCTGAACTCC201800.20489770497309473Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTGGATCCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG200580.20365897751983816TruSeq Adapter, Index 10 (100% over 36bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG199080.20213595196255552No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGGGGG195560.19856191865479886TruSeq Adapter, Index 10 (97% over 45bp)
ACCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC195280.19827762055077275Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTAGATCGGAA195100.19809485748389882No Hit
TTACCGTGACCGATCCCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT193800.19677490200092052Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGGGGGGGGGG191050.19398268847923564TruSeq Adapter, Index 10 (97% over 41bp)
GGATGGGTGACCGCCTGGGAATACCCCGTGTTGTAGGCTTAGATCGGAAG190170.19308918015229645No Hit
AGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC186310.18916992771822239Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTGGATCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA181250.1840322548383222TruSeq Adapter, Index 10 (100% over 35bp)
CTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCC169680.17228465103981525Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGGGCTGAGACTTGAAGCGGTTGAGATCGGAAGAGCACACGTCTGAAC168660.17124899366086302Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAA167390.16995949868903037No Hit
CTTTCCGAGGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT162350.1648421328165606Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTT157260.15967399942551477TruSeq Adapter, Index 10 (100% over 39bp)
GGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTGAAC154030.15639441772549942Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGACCGAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTC152520.15486123866450152Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC146990.1492463511099861No Hit
CGGACCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTAT141580.14375330560005328TruSeq Adapter, Index 10 (100% over 41bp)
TCTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTG135800.13788458045265742Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT130450.1324524559650159Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGACTGGCCGTGGTCGAGGCGAGGGGGGGTTCTCTCTCCCTTTCCGAGG129850.13184324574210285No Hit
GAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTGAACTCCAG129290.13127464953405063Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGACTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG127290.1292439487910071TruSeq Adapter, Index 10 (100% over 36bp)
CTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC124620.12653296329904393Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTAGGGGGGGGGG121510.12337522364361121TruSeq Adapter, Index 10 (97% over 41bp)
ATGGGGGACAAGAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC121490.12335491663618078Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAATCTAGATCGGAA120880.12273555290955249No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC116700.11849138835659145No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAAGATCGGAAGAGCACACGTCTGA115980.11776033608909577Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT115120.11688713476958705No Hit
CTCGGGCTGAGACTTGAAGCGGTAGATCGGAAGAGCACACGTCTGAACTC112030.11374970212158475Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTAATGCTGTAGGTGAAAGACAAGATCGGAAGAGCACACGTCTGAACT111030.11273435175006298Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCTAGATCGGAAGAGCACA109460.1111402516667738No Hit
CGGTTAGGCGAACAAGAATAGAGGCAGATCGGAAGAGCACACGTCTGAAC108210.10987106370237155Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAAC108060.10971876114664329Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCC106700.10833788464137367Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTAGATCGGAAGAG106100.10772867441846061No Hit
ATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGAGC104860.10646963995777362No Hit
AGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTGAACTCCAGT102170.10373834745838002Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCTAGATCGGAAGAGCAC102120.10368757993980393No Hit
CGGTTAGGCGAACAAGAATAGAGGCCAGATCGGAAGAGCACACGTCTGAA101590.10314944424289739Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCA99600.10112889700356906No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)