FastQCFastQC Report
Wed 30 Apr 2025
1A6_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A6_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17047223
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA11066716.491796347123517Illumina DpnII expression Sequencing Primer (100% over 25bp)
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG3158361.8527123156657246Illumina RNA PCR Primer (100% over 37bp)
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG1503300.8818445092200647Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG1272520.7464676211486175Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1242830.7290512947475375No Hit
AGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG1033150.606051789197572Illumina Small RNA Adapter 1 (100% over 26bp)
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACCCATCCGATCGTCGGA963390.5651301681218108No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT954300.5597979213388597Illumina RNA PCR Primer (100% over 50bp)
CAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA953560.5593638330418977Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT894950.5249828667109007Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT864800.5072967016387362Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG827330.4853165820614888Illumina RNA PCR Primer (100% over 36bp)
AGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC740650.4344695907362741Illumina RNA PCR Primer (100% over 30bp)
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA666620.3910431628658814Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACATTCTTGGCAAATGCTTTCGCAGTAGTTCGTCTTTCGATCGTCGGAC653710.38347008190131615No Hit
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC636330.37327487298077816Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGAACG634870.37241842850298845Illumina Small RNA Adapter 1 (100% over 26bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC625220.3667576824682824No Hit
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG591550.3470066649565152Illumina DpnII expression Sequencing Primer (100% over 23bp)
AACATTCTTGGCAAATGCTTTCGCAGTAGTTCGTCTTTGATCGTCGGACT557920.3272791116770163No Hit
AGCCGGGCTGTAACAGTCCGGAGCCGAGACTCCAGGATCGTCGGACTGTA531600.31183964684453297No Hit
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG462610.2713697122399349Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT438620.25729703893707495Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGT437710.25676322765297316Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCGCGGTCCGTTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA437130.2564229962850841Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCT422780.24800520295886314Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTA388790.2280664715889503No Hit
ACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG359840.21108423348483207Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAGTTAAGAGCACACTCGGTCTCGATCGTCGGACTGTAGAACTCTGAACG357190.20952972809706308Illumina Small RNA Adapter 1 (100% over 26bp)
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG340510.1997451432412188Illumina Small RNA Adapter 1 (100% over 26bp)
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAG328070.19244776700580501No Hit
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGAC313870.18411796455058985No Hit
CGGGCCATCCCGAAATCCAACTACGGATCGTCGGACTGTAGAACTCTGAA305320.1791024849032596Illumina DpnII expression Sequencing Primer (100% over 25bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT302540.1774717207606189No Hit
AAGCCTACAACACGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA295090.17310150750066447Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTGTCGGCGCCAGTCAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG293780.17233305389388054Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGAACACCGCCCCAACGACCGGCAGCAGTCGCGGGATCGTCGGACTGTAG268880.15772656930691878No Hit
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC263860.15478180815725823Illumina DpnII expression Sequencing Primer (100% over 26bp)
AAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG256240.1503118719101639Illumina RNA PCR Primer (100% over 37bp)
AACATTCTTGGCAAATGCTTTCGCAGTAGTTCGTCTTTCGGATCGTCGGA252010.14783052934780053No Hit
GAGCTAGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC249840.14655759474725005Illumina RNA PCR Primer (100% over 32bp)
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACCCATCGATCGTCGGAC249150.14615283674062338No Hit
AGTTAAGAGCACACTCGGTCTCGATCGTCGGACTGTAGAACTCTGAACGT248940.14602964952121528Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG247570.14522599956602902Illumina DpnII expression Sequencing Primer (96% over 29bp)
TGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG241650.1417532931903337Illumina DpnII expression Sequencing Primer (96% over 29bp)
TGCACGAGACAGAGTGTCGGCGCCAGTCAGGATCGTCGGACTGTAGAACT239390.14042756406717974No Hit
CAGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAG237900.13955352141518884Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC230250.13506598699389338No Hit
AACAAAGGCTTAATCTCAGAAGATCGTGATCGTCGGACTGTAGAACTCTG228000.13374612392880647Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACAAGGCG227710.13357600824486193No Hit
GGTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTAGAACTCTGA224860.13190418169575185Illumina DpnII expression Sequencing Primer (100% over 24bp)
GACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG223680.1312119868438396Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGAAC222540.13054325622419558Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCCTCGACCACGGCCAGTCCGATCGTCGGACTGTAGAACTCTGAACGTGT221970.13020889091437357Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG220570.12938764278498616No Hit
GACCGCGGTCCGTTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT212300.12453641276353339Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCGACCTCGGAAAGGGAGAGAGAACCCCCCCTCGCCTCGACCACGGCCA210570.12352158471793323No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA210540.12350398654373208Illumina RNA PCR Primer (100% over 45bp)
AGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA209010.12260647965947298Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCACACTCGGTCTCGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTA204540.11998435170350033Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT202410.11873488133521805No Hit
ATCCAACCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGT201720.11833012332859141Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA197890.11608342308891013Illumina DpnII expression Sequencing Primer (100% over 25bp)
TTCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCT197180.11566693296614938Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACAGACCGAAAAACACAGGATGAATCTCAGTGGATCGTATCGATCGTCGG194250.11394817795250288No Hit
ACCGCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGA191240.11218249447431995Illumina DpnII expression Sequencing Primer (100% over 24bp)
AACATTCTTGGCAAATGCTTTCGCAGTAGTTCGTCTTGATCGTCGGACTG190480.11173667406122394No Hit
CGGCAGCAGTCGCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC183710.10776535274982912Illumina RNA PCR Primer (100% over 30bp)
AACATTCTTGGCAAATGCTTTCGCAGTAGTTCGTCGATCGTCGGACTGTA181460.10644548968474221No Hit
GGTCTCGGCGAGGAAGAACAGCTCGGCGATCGTCGGACTGTAGAACTCTG181280.10633990063953526Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTGTCGGCGCCAGTCAGGATCGTCGGACTGTAGAACTCTGAACGTGT176620.1036063175802886Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATTACTTCGGTTCCAGAAACCAACAAAATAGAACGATCGTCGGACTGTAG175970.10322502380593014No Hit
CCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAACG173690.10188756256664208Illumina Small RNA Adapter 1 (100% over 26bp)
GCCAATCCACGGCCGATTCGATCGTCGGACTGTAGAACTCTGAACGTGTA173200.1016001257213565Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCGATCGTCGGACTGT172600.10124816223733332No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)