FastQCFastQC Report
Wed 30 Apr 2025
1A6_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A6_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17047223
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC10444616.126868874772155Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGACAT2884101.6918298071187314TruSeq Adapter, Index 6 (97% over 36bp)
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA1388020.8142205918230788Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1224730.7184337296461717No Hit
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA1192130.6993103803475792Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGTGGTGGACTGCTTGCAGTGCTAGATCGGAAGAGCACACGTCTGAACTC987300.5791559129601344Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGATGGGTGACCGCCTGGGAATACCCCGTGTTGTAGGCTTAGATCGGAAG952060.5584839243318399No Hit
GAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA868040.5091973044524613Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG861620.5054312951734132Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC802070.4704989193841132Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGACA761830.44689390172229226TruSeq Adapter, Index 6 (97% over 36bp)
AGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG687760.40344400961963134Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTAGATCGGAAGA645610.3787185748670033No Hit
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT621690.36468696397061273Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG618420.3627687629826864No Hit
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT612340.3592021996779182Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACTGCTGCCGGTCGTTGGGGCAGATCGGAAGAGCACACGTCTGAACTC605110.35496103969543896Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA562870.3301828104202075Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTAGATCGGAAGAG550300.32280917542992194No Hit
CTGGAGTCTCGGCTCCGGACTGTTACAGCCCGGCTAGATCGGAAGAGCAC522770.30665991757132527No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA445030.26105718215805584Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGAACGGACCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA414120.24292519667279533Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGGGCTGAGACTTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG411130.24117124531074652Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTG406020.23817368963848246Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC399700.23446634094010502Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCAGATCGGAAGAGCAC386670.22682286727873507No Hit
CAGCTGTGACCCTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC338880.1987889757762892Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAGACCGAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTC332700.1951637518908505Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCAGATCGGAAGAGCACA326400.19146813530860715No Hit
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC324320.19024799523066016Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGA305410.1791552794258631No Hit
GGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCG299270.1755535197726926No Hit
CGTAGTTGGATTTCGGGATGGCCCGAGATCGGAAGAGCACACGTCTGAAC285740.16761674320797001Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCCGTGTTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA278140.16315853907700978Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGACTGGCCGTGGTCGAGGCGAGGGGGGGTTCTCTCTCCCTTTCCGAGG275870.16182694389578878No Hit
CTGACTGGCGCCGACACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC275680.16171548879251477Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGCGACTGCTGCCGGTCGTTGGGGCGGTGTTCTAGATCGGAAGAGCACA266500.1563304474869602No Hit
CCTGTGAATAGGCGGTTGCTCACAGCCCGACCAAGATAGTCGAACTCGAC248840.14597098894054475No Hit
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT248650.14585953383727074Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGAAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTAGATCGGAAG248620.14584193566306958No Hit
GATGGGTGACCGCCTGGGAATACCCCGTGTTGTAGGCTTAGATCGGAAGA246800.14477431309486594No Hit
CTCGGGCTGAGACTTGAAGCAAGATCGGAAGAGCACACGTCTGAACTCCA234270.13742414233684866Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGACAT234000.13726575876903824TruSeq Adapter, Index 6 (97% over 36bp)
CGGACTGGCCGTGGTCGAGGTAGATCGGAAGAGCACACGTCTGAACTCCA233890.13720123213030064Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAGACCGAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCC233190.13679060806560694Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGACTGGCGCCGACACTCTGTCTCGTGCAAGATCGGAAGAGCACACGTC232110.13615707379436523No Hit
GGGGGACTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAC231970.13607494898142647TruSeq Adapter, Index 6 (97% over 36bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT227780.13361707065133133No Hit
GCCTTGTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTA225060.1320215028570929No Hit
ACGATCTTCTGAGATTAAGCCTTTGTTAGATCGGAAGAGCACACGTCTGA220230.12918819681070637Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC217540.12761022719066914Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGAGCTGTTCTTCCTCGCCGAGACCAGATCGGAAGAGCACACGTCTGAA216300.12688283599035455Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC210050.12321654969844648Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGACTGCTGCCGGTCGTTGGGGCGAGATCGGAAGAGCACACGTCTGAACT209790.1230640321887031Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAG207930.12197294538823127Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGAACGGACCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC198800.11661723437301195Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCCGAGACCGAGTGTGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT196060.11500993446263946Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC195840.11488088118516429No Hit
CGAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA195080.11443506077206828Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GATACGATCCACTGAGATTCATCCTGTGTTTTTCGGTCTGTAGATCGGAA192900.11315626011345074No Hit
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC192630.11299787654564031No Hit
TGAAAATCTTTGCTCTGAAGTGGATAGATCGGAAGAGCACACGTCTGAAC190900.11198304850004015Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTAGATCGGAAGAGC188280.11044614128647229No Hit
GAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTGAACTCCAG188180.11038748070580176Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTTTCCGAGGTCGCGGTCGGACCGGGAAAGATCGGAAGAGCACACGTCTG187450.10995925846690689Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCTGCCGGTCGTTGGGGCGGTAGATCGGAAGAGCACACGTCTGAA183030.1073664608012695Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACGAACTACTGCGAAAGCATTTGCCAAGAATGTTAGATCGGAAGAGCAC178380.1046387438000899No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACCAGATCGGAAGAGCACACGTCTGA173960.10204594613445252Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCGACTGCTGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG171840.10080234182423731Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTCTATTTTGTTGGTTTCTGGAACCGAAGTAATAGATCGGAAGAGCACA171590.10065569037256097No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCA170790.10018640572719674No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)