FastQCFastQC Report
Wed 30 Apr 2025
1A5_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A5_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19358871
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC70

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG9756155.0396275691903725Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT6402653.307346797238331Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA5862093.0281156375286553Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA3794871.960274439557968Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC2783111.4376406558006405Illumina RNA PCR Primer (100% over 30bp)
GCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGT2626561.356773336626914Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT2317961.1973632140014778Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC2084681.0768603189721135No Hit
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG1897010.9799176821830158Illumina Small RNA Adapter 1 (100% over 26bp)
TCAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA1797340.9284322417355847Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC1656610.8557368867223714Illumina DpnII expression Sequencing Primer (100% over 26bp)
CGCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGAAC1536520.7937033104874763Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG1531500.7911101840598039Illumina DpnII expression Sequencing Primer (100% over 23bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC1437070.7423315130308994Illumina DpnII expression Sequencing Primer (100% over 21bp)
CGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACGT1421200.7341337209179192Illumina DpnII expression Sequencing Primer (96% over 28bp)
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA1227830.6342466975475998Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTAGAACTCTGA1223640.6320823151308772Illumina DpnII expression Sequencing Primer (100% over 24bp)
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA1198720.6192096636213961Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG1025280.5296176621043654Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG995640.5143068518820132Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAG953880.4927353459816949Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCT896630.4631623404071446Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC839520.4336616530995015No Hit
CAGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAG747480.38611755819851273Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT743480.38405132200116426Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCCAGTCCGGGCCGGATCCGATCGTCGGACTGTAGAACTCTGAACGTGT698350.36073901210457987Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA687270.3550155378379245Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG662520.3422307013668307Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTA638630.32989010567816685No Hit
CGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC629660.3252565710056129Illumina RNA PCR Primer (100% over 30bp)
GCGAGCTGCTCCCACTTATTCTACACCTCGATCGTCGGACTGTAGAACTC627300.3240374916491773Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCCAGTCCGGGCCGGATCGATCGTCGGACTGTAGAACTCTGAACGTGTA617830.3191456774519547Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACT602730.31134563580696417No Hit
AGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG601540.310730930538253Illumina Small RNA Adapter 1 (100% over 26bp)
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCGATCGTCGGACTGT544150.2810856066967955No Hit
GACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG520700.26897229698984Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT481630.24879033493223857Illumina RNA PCR Primer (100% over 50bp)
GGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTA475870.24581495480805673No Hit
GGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT466380.24091280942984744Illumina RNA PCR Primer (100% over 31bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA440910.22775605044323088Illumina RNA PCR Primer (100% over 45bp)
CCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGT423440.21873176385131138Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCCAATCCACGGCCGATTCCTTCCCGGGATCGTCGGACTGTAGAACTCT421060.217502353313889Illumina DpnII expression Sequencing Primer (100% over 22bp)
AACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC407650.2105752964622782Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGTCTCGGCGAGGAAGAACAGCTCGGCGATCGTCGGACTGTAGAACTCTG392560.202780420407781Illumina DpnII expression Sequencing Primer (100% over 23bp)
AACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAA364720.18839941647423553Illumina DpnII expression Sequencing Primer (100% over 25bp)
AATCCACGGCCGATTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT364250.18815663372104705Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATTACTTCGGTTCCAGAAACCAACAAAATAGAACGATCGTCGGACTGTAG358860.18537238044511997No Hit
ACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACG357170.18449939565174023Illumina Small RNA Adapter 1 (100% over 26bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG352770.18222653583465687Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAG348480.18001049751300063No Hit
CAGTTAAGAGCACACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC348380.1799588416080669Illumina RNA PCR Primer (100% over 30bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGGATCGTCGGACTGTAGAACT335210.173155758928297No Hit
GCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACGTG332940.17198316988630175Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC326290.16854805220820987Illumina RNA PCR Primer (100% over 32bp)
GGCCAGTCCGGGCCGGATCCAAGATCGTCGGACTGTAGAACTCTGAACGT321320.16598075373300436Illumina DpnII expression Sequencing Primer (96% over 28bp)
ATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCT311800.16106311158331496Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT305850.15798958523975906No Hit
GCCTCGACCACGGCCAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA304120.15709593808440586Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT301180.1555772544793547Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG299950.15494188684867005Illumina RNA PCR Primer (100% over 37bp)
AGCCGGGCTGTAACAGTCCGGAGCCGAGACTCCAGGATCGTCGGACTGTA281550.145437200340867No Hit
GGTTCCAGAAACCAACAAAATAGAACGATCGTCGGACTGTAGAACTCTGA273260.14115492582186223Illumina DpnII expression Sequencing Primer (100% over 24bp)
CAGTTAAGAGCACACTCGGTCTCGATCGTCGGACTGTAGAACTCTGAACG269960.1394502809590497Illumina Small RNA Adapter 1 (100% over 26bp)
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCCAGTAACGTAGATC265850.13732722326627417No Hit
GACAGGGTCGAGTTCGACTATCTTGGTCGATCGTCGGACTGTAGAACTCT259330.13395925826459612Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGCTGCTCCCACTTATTCTACACCTCGATCGTCGGACTGTAGAACTCTGA253610.13100454050238777Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCCTCGACCACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG250250.12926890209661504Illumina RNA PCR Primer (100% over 34bp)
GTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC247840.12802399478771256Illumina RNA PCR Primer (100% over 30bp)
AATCCACGGCCGATTCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA244430.12626252842947297Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTC243110.12558067048434798Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT242270.1251467608829048Illumina RNA PCR Primer (100% over 35bp)
TCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGA239400.12366423641130724Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCAATCCACGGCCGATTCGATCGTCGGACTGTAGAACTCTGAACGTGTA238110.12299787523766235Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTG237310.12258462799819266Illumina DpnII expression Sequencing Primer (96% over 29bp)
GACCGCGGTCCGTTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT236590.12221270548266994Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTAGAACTCTGAA219860.11357067258725986Illumina DpnII expression Sequencing Primer (100% over 25bp)
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG214300.11069860427294546Illumina RNA PCR Primer (100% over 36bp)
AGTTAAGAGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGGATCGTCG213680.11037833766235644No Hit
GGCCAGTCCGGGCCGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA212950.11000124955634034Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT208570.10773872092024375Illumina RNA PCR Primer (100% over 31bp)
AGCCTTAACTCCAGAAAGTGGATCGTCGGACTGTAGAACTCTGAACGTGT208170.10753209730050889Illumina DpnII expression Sequencing Primer (96% over 30bp)
TAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA207610.10724282423288012Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT203550.10514559449257138Illumina RNA PCR Primer (100% over 31bp)
GCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGT202240.10446890213793975Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCCAGTCCGGGCCGGATCCAGATCGTCGGACTGTAGAACTCTGAACGTG201570.10412280757488387Illumina DpnII expression Sequencing Primer (96% over 29bp)
GACCGCGGTCCGTTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA200840.10374571946886778Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCAGTTAAGAGCACACGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT196370.10143670051833084Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGAC194690.10056888131544449No Hit
TCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAACTC193620.10001616313265375Illumina DpnII expression Sequencing Primer (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)