FastQCFastQC Report
Wed 30 Apr 2025
1A5_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A5_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19358871
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC75

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA9032394.665762791642136Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5914333.0551006822660267Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5332902.7547577542099435Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC3558941.8384026630478605Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC2574281.329767629527569Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGGGCTGAGACTTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG2456201.2687723369818416Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2109571.0897174737101147Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG2050931.0594264510569857No Hit
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC1795540.9275024354467778Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTGTGCTCTTAACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1654520.8546572783092568Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT1575110.8136373242013958Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA1459110.7537164744782896Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCTGCCGGTCGTTGGGGCGAGATCGGAAGAGCACACGTCTGAACT1438230.7429307215281304Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCT1397710.7219997488489902Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCC1318080.6808661517502751Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGAGCTGTTCTTCCTCGCCGAGACCAGATCGGAAGAGCACACGTCTGAA1182660.610913725289042Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT1128580.5829782119008903Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC1123410.5803076016158174Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA954070.492833492201069Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGCTGTGACCCTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC923790.4771920841871408Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAGTGTGCTCTTAACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC879950.4545461354642014Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTTTCCGAGGTCGCGGTCGGACCGGGAAAGATCGGAAGAGCACACGTCTG846950.4374996868360763Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT825580.42646081995174207No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG713480.3685545505210506Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC684080.3533677144705391Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG644190.33276217399248126Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA640010.3306029571662521Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGGGCTGAGACTTGAAGCAAGATCGGAAGAGCACACGTCTGAACTCCA636760.3289241402559065Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCAC634780.32790135333821896No Hit
GAGGTGTAGAATAAGTGGGAGCAGCTCGCAGATCGGAAGAGCACACGTCT593670.30666561081996985Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTTCCGAGGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC581330.3002912721511497Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGACCAAGATAGTCGAACTCGACCCTGTCAAGATCGGAAGAGCACACGTC580850.3000433238074679No Hit
CGTGGTGGACTGCTTGCAGTGCTAGATCGGAAGAGCACACGTCTGAACTC570070.2944748172556137Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT566770.29277017239280123Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCA537600.2777021449236374No Hit
CTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC488020.25209114725750276Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCAGATCGGAAGAGCAC474090.24489547970023667No Hit
GTTGGATCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA435820.22512676488210492TruSeq Adapter, Index 7 (100% over 35bp)
CCGGGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTG402140.20772905610043066Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCC394220.20363790842968066Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACCAAGATAGTCGAACTCGACCCTGTTAGATCGGAAGAGCACACGTCT389620.20126173680272988Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTGGGGGG389320.20110676908792874TruSeq Adapter, Index 7 (97% over 36bp)
GCCGAGCTGTTCTTCCTCGCCGAGACCAGATCGGAAGAGCACACGTCTGA374550.19347719192921942Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTCTATTTTGTTGGTTTCTGGAACCGAAGTAATAGATCGGAAGAGCACA349320.18044440711444382No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTGGGGGGGGGGG348680.18011380932286805TruSeq Adapter, Index 7 (96% over 32bp)
TCTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAAC346930.17920983098652807Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCAGATCGGAAGAGCACA345780.1786157880797904No Hit
CTCGGGCTGAGACTTGAAGCGGTAGATCGGAAGAGCACACGTCTGAACTC338780.17499987473443054Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA333450.17224661500146368Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAATCGGCCGTGGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC333440.1722414494109703Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTC328760.16982395306007259No Hit
GTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC313480.16193093078620133Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTCGGGCTGAGACTTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCA312420.16138337819390397Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTGGATCCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG303310.15667752525444278TruSeq Adapter, Index 7 (100% over 36bp)
TTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCC301650.15582003723254315Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG301250.1556134136128083No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTG297900.15388294079752896Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGTGTGCTCTTAACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC287920.14872768148514445Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA286260.14787019346324481Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGGAGTCTCGGCTCCGGACTGTTACAGCCCGGCTAGATCGGAAGAGCAC275830.14248248257865864No Hit
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGAT275770.1424514890356984TruSeq Adapter, Index 7 (97% over 36bp)
TACGTTACTGGCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGAT269770.13935213473967567No Hit
GTTCTATTTTGTTGGTTTCTGGAACCAGATCGGAAGAGCACACGTCTGAA258980.13377846259732812Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCTG254000.13120599853162926Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGACCGAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTC253020.13069977066327887Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGGTGTAGAATAAGTGGGAGCAGCTAGATCGGAAGAGCACACGTCTGAA238690.12329747948627788Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGC230880.11926315331095495TruSeq Adapter, Index 7 (97% over 36bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT229770.11868977276619076Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGTGTGCTCTTAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC229710.11865877922323054Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAATCGGCCGTGGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA224280.11585386358532995Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCG223420.11540962280290003TruSeq Adapter, Index 7 (97% over 36bp)
CGAACGGACCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC221810.11457796273346725Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTGGATCCGGCCCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA221210.114268027303865Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTTTCCGAGGTCGCGGTCGGAAGATCGGAAGAGCACACGTCTGAACTCCA220810.11406140368413013Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAATCGGCCGTGGATTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT219540.113405373691472Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTCTTAACTAGATCGGA213140.1100993957757144No Hit
CCGAGCTGTTCTTCCTCGCCGAGACAGATCGGAAGAGCACACGTCTGAAC211140.10906627767704015Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA199320.10296054971387536Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGA197390.10196359074865471TruSeq Adapter, Index 7 (97% over 36bp)
GAGTGTGCTCTTAACTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA196760.10163815854757233Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CACTTTCTGGAGTTAAGGCTAGATCGGAAGAGCACACGTCTGAACTCCAG196140.1013178919369833Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)