FastQCFastQC Report
Wed 30 Apr 2025
1A4_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A4_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13294987
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG4282763.2213344774237087Illumina Small RNA Adapter 1 (100% over 26bp)
CGGGCCATCCCGAAATCCAACTACGGATCGTCGGACTGTAGAACTCTGAA1863551.4016937361428035Illumina DpnII expression Sequencing Primer (100% over 25bp)
AACATTCTTGGCAAATGCTTTCGCAGTAGTTCGTCTTTCGATCGTCGGAC1862181.4006632725552872No Hit
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA1469691.1054467371799612Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACATTCTTGGCAAATGCTTTCGCAGTAGTTCGTCTTTGATCGTCGGACT1361471.0240476353982144No Hit
ACCTACCTTCCACGGCACCTCCGACGCCCAGTTCGATCGTCGGACTGTAG767000.5769091763685065No Hit
AACATTCTTGGCAAATGCTTTCGCAGTAGTTCGTCGATCGTCGGACTGTA684140.5145849334038461No Hit
ATTACTTCGGTTCCAGAAACCAACAAAATAGGATCGTCGGACTGTAGAAC642890.48355820129797794No Hit
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG578090.43481802577166867Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG570220.4288985013674703Illumina DpnII expression Sequencing Primer (96% over 29bp)
ATTACTTCGGTTCCAGAAACCAACAAAATAGAACGATCGTCGGACTGTAG565790.4255664183801007No Hit
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG531280.39960926625953075Illumina DpnII expression Sequencing Primer (100% over 23bp)
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC477350.3590451047451193Illumina DpnII expression Sequencing Primer (100% over 26bp)
TGTCGGCGCCAGTCAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT468650.35250128488279076Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATCATTACTTCGGTTCCAGAAACCAACAAAATAGGATCGTCGGACTGTA447600.33666824946876595No Hit
AACATTCTTGGCAAATGCTTTCGCAGTAGTTCGTCTTTCGGATCGTCGGA430450.3237686505447504No Hit
AACATTCTTGGCAAATGCTTTCGCAGTAGTTCGTCTTGATCGTCGGACTG406360.3056490389949234No Hit
GGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG398910.300045423135803Illumina Small RNA Adapter 1 (100% over 26bp)
ACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACGTGT393980.29633725854715015Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGAACACCGCCCCAACGACCGGCAGCAGTCGCGGGATCGTCGGACTGTAG393240.29578065777725093No Hit
GCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGT389430.2929149159754726Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTCTTGTCCCCCATGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC387580.2915234140507245Illumina RNA PCR Primer (100% over 30bp)
CGGGCCATCCCGAAATCCAACTACGAGCTTTTTAACGATCGTCGGACTGT378760.284889334604088No Hit
AGCCATTCGCAGTTTCACAGTACTAGGATCGTCGGACTGTAGAACTCTGA356480.26813113845090636Illumina DpnII expression Sequencing Primer (100% over 24bp)
GGCTCTTGTCCCCCATGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT356100.26784531643393106Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCTCTATTCTTGTTCGCCTAACCGGATCGTCGGACTGTAGAACTCTGA354110.26634851166082374Illumina DpnII expression Sequencing Primer (100% over 24bp)
ACCGCCCCAACGACCGGCAGCAGTCGCGGGATCGTCGGACTGTAGAACTC335110.25205741081206023Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAAC334350.25148576677810963Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT312170.2348027869451847Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTGTCGGCGCCAGTCAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG305800.23001150734483608Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCGCGGTCCGTTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT300760.2262206048039009Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATTACTTCGGTTCCAGAAACCAACAAAATAGAGATCGTCGGACTGTAGAA295970.22261774306360735No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT289570.21780389856718174Illumina RNA PCR Primer (100% over 50bp)
ACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG287020.2158858824006372Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCT277730.20889828624879436Illumina DpnII expression Sequencing Primer (100% over 22bp)
CAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA269950.203046456532827Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTTAAGAGCACACTCGGTCTCGATCGTCGGACTGTAGAACTCTGAACGT265660.1998196763938167Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAAAGGCTTAATCTCAGAAGATCGTGATCGTCGGACTGTAGAACTCTG265000.1993232486801228Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCCTCTATTCTTGTTCGCCTAACCGTGATCGTCGGACTGTAGAACTCTG263120.1979091818592978Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCCGGGCTGTAACAGTCCGGAGCCGAGACTCCAGGATCGTCGGACTGTA262500.19744284067370657No Hit
TCCAACCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGTA255620.19226795784004902Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCGCGGTCCGTTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA248870.18719085622272516Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT242000.1820234950210933Illumina RNA PCR Primer (100% over 44bp)
ATCCAACCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGT240460.18086516368914088Illumina DpnII expression Sequencing Primer (96% over 30bp)
TATCCCCAGCACGACGACGGATCGTCGGACTGTAGAACTCTGAACGTGTA237330.17851089286510774Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATCATTACTTCGGTTCCAGAAACCAACAAAATAGAGATCGTCGGACTGT221130.16632584898353042No Hit
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGAC216720.16300880926021213No Hit
CAGTTAAGAGCACACTCGGTCTCGATCGTCGGACTGTAGAACTCTGAACG209010.1572096309684244Illumina Small RNA Adapter 1 (100% over 26bp)
GCCTCTATTCTTGTTCGCCTAACCGGATCGTCGGACTGTAGAACTCTGAA207900.15637472981357559Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGCTTTTACCCTTTTGTTCTACGCGGATCGTCGGACTGTAGAACTCTGAA206980.1556827396672144Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACAAAGGCTTAATCTCAGAAGATCGTGATCGTCGGACTGTAGAACTCTGA206340.15520135521757186Illumina DpnII expression Sequencing Primer (100% over 24bp)
AACATTCTTGGCAAATGCTTTCGCAGTAGTTCGGATCGTCGGACTGTAGA205400.15449432180715933No Hit
TGGGCCATCCCGAAATCCAACTACGGATCGTCGGACTGTAGAACTCTGAA205240.1543739756947487Illumina DpnII expression Sequencing Primer (100% over 25bp)
AACAGTCGGATTCCCCTAGTCACTGCCAGTTCGATCGTCGGACTGTAGAA205030.15421602142220975No Hit
TATCCCCAGCACGACGACGTTTATCGATCGTCGGACTGTAGAACTCTGAA204810.1540505455176451Illumina DpnII expression Sequencing Primer (100% over 25bp)
ATTAATGAAAACATTCTTGGCAAATGCTTTCGCAGTAGTTCGTCTTTCGA202670.15244091626415282No Hit
AACATTCTTGGCAAATGCTTTCGCAGTAGTTCGTCTGATCGTCGGACTGT195560.147093035893905No Hit
GCCAATCCACGGCCGATTCGATCGTCGGACTGTAGAACTCTGAACGTGTA187810.1412637710740146Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCCAACGACCGGCAGCAGTGATCGTCGGACTGTAGAACTCTGAACGTG180320.1356300686867915Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC178460.1342310451300178Illumina RNA PCR Primer (100% over 30bp)
AACATTCTTGGCAAATGCTTTCGCAGTAGTTCGTCTTTCGCGATCGTCGG176540.1327868917810901No Hit
GACCCGAGGGTCACAGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA164800.12395649578295939Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG159430.1199173793851773Illumina Small RNA Adapter 1 (100% over 26bp)
AAGCCTACAACACGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA151800.11417837414959488Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAGATCCCAAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTA149060.11211744697456266Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCGCAGATCCCAAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTG148060.11136528377199617Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTGCTTCTCGCAACTACAACTCGCCGATGCAGGCAGATCGTCGGACTGT145780.10965035167014454No Hit
TGCACGAGACAGAGTGTCGGCGCCAGTCAGGATCGTCGGACTGTAGAACT143490.10792789793626725No Hit
GGTTCCAGAAACCAACAAAATAGGATCGTCGGACTGTAGAACTCTGAACG141890.10672443681216084Illumina Small RNA Adapter 1 (100% over 26bp)
AGAGTGTCGGCGCCAGTCAGGATCGTCGGACTGTAGAACTCTGAACGTGT140020.10531789162336148Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCGCAGATCCCAAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGT136920.10298618569540535Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGCTTCTCGCAACTACAACTCGCCGATGCAGGCAGATCGTCGGACTGTAG135810.10215128454055653No Hit
AATGCTTTCGCAGTAGTTCGTCTTTCGATCGTCGGACTGTAGAACTCTGA135750.10210615474840254Illumina DpnII expression Sequencing Primer (100% over 24bp)
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACCCATCCGATCGTCGGA133000.10003770594134466No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)