FastQCFastQC Report
Wed 30 Apr 2025
1A4_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A4_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13294987
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGTGGACTGCTTGCAGTGCTAGATCGGAAGAGCACACGTCTGAACTC4168083.1350764013533823Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTAGATCGGAAGA1835841.3808512937996855No Hit
CGTAGTTGGATTTCGGGATGGCCCGAGATCGGAAGAGCACACGTCTGAAC1783901.3417839370583815Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT1387361.0435211407126612Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTAGATCGGAAGAG1343101.0102303973670677No Hit
GAACTGGGCGTCGGAGGTGCCGTGGAAGGTAGGTAGATCGGAAGAGCACA756060.5686805109324289No Hit
GACGAACTACTGCGAAAGCATTTGCCAAGAATGTTAGATCGGAAGAGCAC675590.5081539380219025No Hit
CTATTTTGTTGGTTTCTGGAACCGAAGTAATAGATCGGAAGAGCACACGT630580.4742990722743843No Hit
GTTCTATTTTGTTGGTTTCTGGAACCGAAGTAATAGATCGGAAGAGCACA555560.41787178881784537No Hit
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA555220.41761605332897284Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA542270.4078755398557366Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA513970.38658932122310463Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT465740.35031248996332226Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTGACTGGCGCCGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC448610.3374279343033581Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTATTTTGTTGGTTTCTGGAACCGAAGTAATGATTAGATCGGAAGAGCAC438960.3301695593985914No Hit
CGAAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTAGATCGGAAG424570.31934593091365937No Hit
CGTGGTGGACTGCTTGCAGTGCCAGATCGGAAGAGCACACGTCTGAACTC406710.3059122961158217Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTAGATCGGAAGAGC401420.3019333527742449No Hit
CCGCGACTGCTGCCGGTCGTTGGGGCGGTGTTCTAGATCGGAAGAGCACA391450.2944342856446569No Hit
CGTGGTGGACTGCTTGCAGTAGATCGGAAGAGCACACGTCTGAACTCCAG377570.2839942603930339Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGGGCTGAGACTTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG374360.2815798165127954Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATGGGGGACAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA371880.2797144517704304Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTAAAAAGCTCGTAGTTGGATTTCGGGATGGCCCGAGATCGGAAGAGCA370560.2787215963430427No Hit
CGGTTAGGCGAACAAGAATAGAGGCCAGATCGGAAGAGCACACGTCTGAA352490.265130007272666Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTAGTACTGTGAAACTGCGAATGGCTAGATCGGAAGAGCACACGTCTGAA348330.26200100834998935Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGGGGGACAAGAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC344530.25914278818023667Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGCGACTGCTGCCGGTCGTTGGGGCGGTAGATCGGAAGAGCACACGTCT331500.24934210165079512Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGTGGACTGCTTGCAGTGCTCAGATCGGAAGAGCACACGTCTGAACT328520.24710065530714698Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC296460.22298630303286496Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGACTGGCGCCGACACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC293790.22097802728201238Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCTATTTTGTTGGTTTCTGGAACCGAAGTAATAGATCGGAAGAGCACACG290290.21834545607302963No Hit
CGAACGGACCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC289850.21801450426390034Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAGCTGTGACCCTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC274840.20672453459337717Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTG271880.20449813151378035Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGTTAGGCGAACAAGAATAGAGGCCAGATCGGAAGAGCACACGTCTGA261810.19692384806393567Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGATCTTCTGAGATTAAGCCTTTGTTAGATCGGAAGAGCACACGTCTGA259580.19524652412221238Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGGAGTCTCGGCTCCGGACTGTTACAGCCCGGCTAGATCGGAAGAGCAC258920.1947500964085185No Hit
GAGACCGAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCC256480.19291481819425624Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA253170.19042515799376108Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT243260.1829712206563271Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGAACGGACCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA242420.18233940356617123Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTGAACTCCAG229970.17297497169421827Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGTCGTCGTGCTGGGGATAAGATCGGAAGAGCACACGTCTGAACTCCAGT224930.16918406915328313Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCTATTTTGTTGGTTTCTGGAACCGAAGTAATGATTAGATCGGAAGAGCA216800.16306898231641748No Hit
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGA208020.15646498939788359No Hit
CGTAGTTGGATTTCGGGATGGCCCAAGATCGGAAGAGCACACGTCTGAAC205330.15444167038297968Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGAACTACTGCGAAAGCATTTGCCAAGAATGTTAGATCGGAAGAGCACAC203340.15294486560987236No Hit
CGGTTAGGCGAACAAGAATAGAGGCAGATCGGAAGAGCACACGTCTGAAC203300.1529147790817697Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGATCTTCTGAGATTAAGCCTTTGTAGATCGGAAGAGCACACGTCTGAA203100.1527643464412564Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGTAGAACAAAAGGGTAAAAGCTAGATCGGAAGAGCACACGTCTGAAC202560.15235817831187048Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGACCGAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTC200590.15087641680281447Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAACTGGCAGTGACTAGGGGAATCCGACTGTTAGATCGGAAGAGCACACG200390.15072598416230118No Hit
GAAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATAG199870.15033485929696658No Hit
GATAAACGTCGTCGTGCTGGGGATAAGATCGGAAGAGCACACGTCTGAAC197990.14892079247614157Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTAGATCGGAAGAGCA193280.14537810379205335No Hit
GAATCGGCCGTGGATTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT177760.13370453088822126Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGAAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTAGATCGGAA174300.13110204620734117No Hit
ACTGCTGCCGGTCGTTGGGGCAGATCGGAAGAGCACACGTCTGAACTCCA174190.13101930825505884Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA171390.12891325128787265Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTGGTGGACTGCTTGCAGTGCAAGATCGGAAGAGCACACGTCTGAACTC160170.1204739801550765Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA155270.11678838046250063Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCCGTGTTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA146510.1101994308080181Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGCCTGCATCGGCGAGTTGTAGTTGCGAGAAGCACTAGATCGGAAGAGCA144030.10833406606565316No Hit
GAGTGTGCTTGGGATCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGT143020.107574381231061Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAGTGTGCTTGGGATCTGCGAAGATCGGAAGAGCACACGTCTGAACTCCA141820.1066717853879812Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGACTGGCGCCGACACTCTGTCTCGTGCAAGATCGGAAGAGCACACGTC141290.10627313889062094No Hit
CTATTTTGTTGGTTTCTGGAACCAGATCGGAAGAGCACACGTCTGAACTC139380.10483650717371894Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGACTGGCGCCGACACTCTAGATCGGAAGAGCACACGTCTGAACTCCAG135240.10172255151509363Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGCCTGCATCGGCGAGTTGTAGTTGCGAGAAGCAAGATCGGAAGAGCACA133710.10057174181516688No Hit
GAAAGACGAACTACTGCGAAAGCATTAGATCGGAAGAGCACACGTCTGAA133260.10023326837401195Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)