FastQCFastQC Report
Wed 30 Apr 2025
1A3_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A3_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38533665
Total Bases5.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC72

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT922267623.93407426986247Illumina RNA PCR Primer (100% over 50bp)
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG30690057.96447729537276Illumina RNA PCR Primer (100% over 37bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA25445476.6034388371830195Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC5783161.5008071513571315No Hit
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG4966371.2888392526379207Illumina RNA PCR Primer (100% over 36bp)
AAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG3620930.9396796281900514Illumina RNA PCR Primer (100% over 37bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC3573080.9272619150034132No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2932400.7609969101044503No Hit
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG1910590.49582358698556184No Hit
TAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG1887990.48995858556407756Illumina RNA PCR Primer (100% over 37bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1608260.41736491973966144No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1438920.37341893121248654No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT1431760.3715608157178924No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT1252240.3249729814176772Illumina DpnII expression Sequencing Primer (100% over 22bp)
CCACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGT1116870.2898426609563352Illumina DpnII expression Sequencing Primer (96% over 28bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1107270.28735133291889053Illumina RNA PCR Primer (100% over 45bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA965600.2505860784329754No Hit
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG965000.25043037043063515Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT776320.2014653939613582Illumina RNA PCR Primer (98% over 50bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGAC747980.19411078598415177No Hit
AAACTAACCAGCCTAGACTTGAGATCGTCGGACTGTAGAACTCTGAACGT709700.1841766154348412Illumina DpnII expression Sequencing Primer (96% over 28bp)
ATCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA699960.18164895553018381Illumina DpnII expression Sequencing Primer (100% over 25bp)
AAGCTCAGAGCATGTTTCTTATCACTGGACATCAGCGTATATTAAAGACA651340.16903141707387553No Hit
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA648040.16817502306100393Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC646000.16764561585304694No Hit
ATGTGTTCTTATTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT640510.16622088763163329Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGCTAGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC625060.16221140657137076Illumina RNA PCR Primer (100% over 32bp)
CGCGACCTCGGAAAGGGAGAGAGAACCCCCCCTCGCCTCGACCACGGCCA622380.16151591082758412No Hit
AAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG601230.15602720374508885Illumina RNA PCR Primer (100% over 36bp)
ACAGACCGAAAAACACAGGATGAATCTCAGTGGATCGTATCGATCGTCGG591500.15350213897380383No Hit
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC590640.15327895750378273Illumina DpnII expression Sequencing Primer (100% over 26bp)
GATATATGGGAATTTGCAGGGCAGCACCTAGATCGTCGGACTGTAGAACT585840.1520332934850604No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTTGCCGTATCATT552570.14339928475529123Illumina RNA PCR Primer (98% over 50bp)
ACCGTCAAAAGTTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC513770.13333016727061908Illumina RNA PCR Primer (100% over 32bp)
ACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGTGT506010.13131634377368465Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCTTCCTTAGGGAGTTACACAAGATCGTCGGACTGTAGAACTCTGAACGT495790.12866411746715503Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGGTCCGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT478070.12406554113137175Illumina RNA PCR Primer (100% over 35bp)
AGATTAAGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGG477700.12396952119659524No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGTCGTATCATT475590.12342194805503189Illumina RNA PCR Primer (98% over 50bp)
AGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG453540.11769967896902618Illumina Small RNA Adapter 1 (100% over 26bp)
CCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAACG408830.10609683766130214Illumina Small RNA Adapter 1 (100% over 26bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT402380.104422976636144No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT400030.10381312029364453No Hit
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC393180.10203545393359288No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)