FastQCFastQC Report
Wed 30 Apr 2025
1A3_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A3_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38533665
Total Bases5.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC73

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGGGGGG531105713.782901263090338TruSeq Adapter, Index 3 (96% over 33bp)
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGAT28033057.274950358342504TruSeq Adapter, Index 3 (97% over 37bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC23774236.169729767464371Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGGGGGT8144782.113679038835263TruSeq Adapter, Index 3 (96% over 33bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT5582491.448730609974421No Hit
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGA4578751.1882466928593478TruSeq Adapter, Index 3 (97% over 37bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGGGGTG3941001.0227420620384797TruSeq Adapter, Index 3 (96% over 33bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG3525880.915012885485977No Hit
CGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGAT3301550.8567962585443144TruSeq Adapter, Index 3 (97% over 37bp)
CGGACCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGAT1765010.45804363535106246TruSeq Adapter, Index 3 (97% over 37bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1561760.40529754955828884No Hit
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC1465040.38019741958103387No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGGGGTT1432820.37183589985536025TruSeq Adapter, Index 3 (96% over 33bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG1414090.3669752150489708No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1380380.3582270204508188No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGGTGGG1367520.35488967893399187TruSeq Adapter, Index 3 (96% over 33bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGGGGGC1343090.34854976810536964TruSeq Adapter, Index 3 (96% over 33bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG1187450.3081591122982981Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC1044760.27112915420840455Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGGCGGG995610.25837407368336235TruSeq Adapter, Index 3 (96% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGG991210.2572322149995335TruSeq Adapter, Index 3 (97% over 37bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGGAGGG981070.25460074975998265TruSeq Adapter, Index 3 (96% over 33bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG924830.24000571967395262No Hit
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA897250.2328483418330439Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGCAATGATGTCTTTAATATACGCTGATGTCCAGTGATAAGAAACATGC802910.20836585359840543No Hit
CGGACTGGCCGTGGTCGAGGCGAGGGGGGGTTCTCTCTCCCTTTCCGAGG778710.202085630837347No Hit
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGA705460.1830762788849698No Hit
TGAAAATCTTTGCTCTGAAGTGGATAGATCGGAAGAGCACACGTCTGAAC673020.17465766622510473Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGTGGGG664350.17240768559128752TruSeq Adapter, Index 3 (96% over 33bp)
TCAAGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC661710.17172257038099023Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT636560.16519580994955968No Hit
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT594660.15432220111946268Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTGAATAAGAACACATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC591610.15353068544089954Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATACGATCCACTGAGATTCATCCTGTGTTTTTCGGTCTGTAGATCGGAA582380.1511353773382314No Hit
GGGGGACTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTA576370.14957570218145613TruSeq Adapter, Index 3 (97% over 36bp)
TAGGTGCTGCCCTGCAAATTCCCATATATCAGATCGGAAGAGCACACGTC559110.14509650198080043No Hit
CCGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGA552550.14339409448854656TruSeq Adapter, Index 3 (97% over 37bp)
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT547380.14205241053504772Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGGTGGT487520.12651794216823134TruSeq Adapter, Index 3 (96% over 33bp)
ACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG476610.12368665165901037Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACTTTTGACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTA476480.12365291492516998TruSeq Adapter, Index 3 (97% over 36bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAATCTAGATCGGAA470290.12204652736769264No Hit
CCTGTGAATAGGCGGTTGGTCACAGCCCGATCAAGATAGTCGAACTCGAC460950.11962267279792876No Hit
TTGTGTAACTCCCTAAGGAAGGAGATCGGAAGAGCACACGTCTGAACTCC446260.11581042187396398Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAG443220.11502150132877316TruSeq Adapter, Index 3 (97% over 37bp)
GGAAGAGCACACGTCTAAACTCCAGTCACGTAGAGATCTGGGGGGGGGGG442590.11485800792631586TruSeq Adapter, Index 18 (96% over 31bp)
CCTGTGAATAGGCGGTTGCTCACAGCCCGACCAAGATAGTCGAACTCGAC440820.114398669319412No Hit
CGTGGTGGACTGCTTGCAGTGCTAGATCGGAAGAGCACACGTCTGAACTC426710.11073693613104282Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGGGAGG404080.1048641493094415TruSeq Adapter, Index 3 (96% over 33bp)
GGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCG391860.10169289632844423No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC386600.10032785617459435No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)