FastQCFastQC Report
Thu 20 Feb 2025
POC-57-TP2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-57-TP2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19127304
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCTTATATCTAGTAT289350.15127589335120098TruSeq Adapter, Index 9 (97% over 37bp)
GGGACTTCTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTTAA234410.12255255628289277No Hit
CGGGATACCATTCATACCGGTCAACAATTAATTGACAATTGATTACGCTA193610.10122179267919827No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)