FastQCFastQC Report
Thu 20 Feb 2025
POC-52-TP1_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-52-TP1_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16402600
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGACTTCTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTTAA1072940.6541280040969114No Hit
GCAGGTTTCACCTTTTATAAAAATCAAAAAGCTATGTTTTTGGTAAACAG783580.4777169473132308No Hit
GGGGACTTCTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTTA760720.46378013241803134No Hit
GTTTTTGGTAAACAGTCGAAGTTTAACTTTGCCGAGTTCCTTAAAAAAAT553940.33771475253923156No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTCTCTAATCTGGGGG474230.28911879823930353TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTCTCTAATCTAGGGG433020.2639947325424018TruSeq Adapter, Index 27 (97% over 37bp)
GGACTTCTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTTAAT370690.2259946593832685No Hit
GGCAGGTTTCACCTTTTATAAAAATCAAAAAGCTATGTTTTTGGTAAACA327270.19952324631460866No Hit
CGGGATACCATTCATACCGGTCAACAATTAATTGACAATTGATTACGCTA234570.1430078158340751No Hit
CGGCCATTTAATTGTCTTTATTAGTAAGCGGAACAAACATATTAAGATTC228000.13900235328545474No Hit
CTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATT224330.1367649031251143No Hit
TTCTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCA222370.13556997061441478No Hit
CGGGCAGGTTTCACCTTTTATAAAAATCAAAAAGCTATGTTTTTGGTAAA215200.13119871239925376No Hit
ATTTAATTGTCTTTATTAGTAAGCGGAACAAACATATTAAGATTCAACCA213180.12996720032190018No Hit
TAACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATAT203920.12432175386828917No Hit
AGGACGAGGATGGAGATTATGAAGAGCTGATGCTCGCCCTCCCGTCCCAG202840.12366332166851596No Hit
GGCTAGATGAGCAGAGAAGACCCCCGAAGAGCATCACCCCAACCCCAAAG200200.12205382073573702No Hit
AAACAGTCGAAGTTTAACTTTGCCGAGTTCCTTAAAAAAATGTTTTTCTT178990.10912294392352433No Hit
CAGGTTTCACCTTTTATAAAAATCAAAAAGCTATGTTTTTGGTAAACAGT170750.10409935010303245No Hit
CTGGTTCCAAGCCTACATTTTTATTGTCTAAATTTAATTTTTTTTTAAAC165370.1008193822930511No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)