FastQCFastQC Report
Thu 20 Feb 2025
POC-42-TP4_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-42-TP4_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16179587
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG711750.4399061607691222No Hit
GAAAAACATTTTTTTAAGGAACTCGGCAAAGTTAAACTTCGACTGTTTAC587550.3631427674884408No Hit
ATTTTTTTAAGGAACTCGGCAAAGTTAAACTTCGACTGTTTACCAAAAAC349590.2160685560144397No Hit
AAAAGAACTGTTTTTCTAATTAAAGAAAGAAAAAAAAAAAGAAAAACATT300580.18577730074321427No Hit
AAAGAACTGTTTTTCTAATTAAAGAAAGAAAAAAAAAAAGAAAAACATTT289440.17889208173237056No Hit
CAAAAACATAGCTTTTTGATTTTTATAAAAGGTGAAACCTGCCCGATGGT282270.1744605718304182No Hit
AAAAAAAAGAACTGTTTTTCTAATTAAAGAAAGAAAAAAAAAAAGAAAAA277180.17131463244395548No Hit
GTTGTAATAATTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAA273820.16923794161124137No Hit
CGATGGTTGAATCTTAATATGTTTGTTCCGCTTACTAATAAAGACAATTA246480.15234010608552614No Hit
GTTGAATCTTAATATGTTTGTTCCGCTTACTAATAAAGACAATTAAATGG202850.12537402839763462No Hit
AAAAAAGAAAAACATTTTTTTAAGGAACTCGGCAAAGTTAAACTTCGACT173410.1071782611014731No Hit
AAAAAAAGAAAAACATTTTTTTAAGGAACTCGGCAAAGTTAAACTTCGAC168480.10413121175466344No Hit
GTTTCTAAAAAAGAAATTTTTTATGAAGATTTTTTATAAAAATATATAAT166820.10310522759326304No Hit
AAAAAAAGAACTGTTTTTCTAATTAAAGAAAGAAAAAAAAAAAGAAAAAC163220.10088020170106937No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)