FastQCFastQC Report
Thu 20 Feb 2025
POC-255-TP3_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-255-TP3_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24339256
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1008320.4142772482445643No Hit
GTTGTAATAATTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAA741850.3047956765810754No Hit
GTTTTTTAAAAAGTAACGAAAATGAGCTATGACGCATGTTTAACTTTGAA529240.2174429653889174No Hit
CGATGGTTGAATCTTAATATGTTTGTTCCGCTTACTAATAAAGACAATTA474040.19476355398866751No Hit
GAAAAACATTTTTTTAAGGAACTCGGCAAAGTTAAACTTCGACTGTTTAC448950.18445510413301047No Hit
CAAAAACATAGCTTTTTGATTTTTATAAAAGGTGAAACCTGCCCGATGGT443400.18217483722592012No Hit
TTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTA439080.18039992676850927No Hit
GTTGAATCTTAATATGTTTGTTCCGCTTACTAATAAAGACAATTAAATGG419180.17222383461515833No Hit
ATTTTTTTAAGGAACTCGGCAAAGTTAAACTTCGACTGTTTACCAAAAAC351780.1445319446083315No Hit
CGAAAATGAGCTATGACGCATGTTTAACTTTGAAATTTTTTTATTGATGA314130.12906310694131323No Hit
CGCATGTTTAACTTTGAAATTTTTTTATTGATGAGACATTTTTGGTGTGT301640.12393147925310453No Hit
GTTTTGTTGGGGCGACAGTTTTTTAAAAAGTAACGAAAATGAGCTATGAC296060.12163888657894883No Hit
TTGTAATAATTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAAG293500.12058708778937204No Hit
GGATAACTAAGATAAACTAAAATTAGACGATTTTTTTCGAAATGGCGTTC283970.1166716024516115No Hit
GTTCAGTTGTAATAATTTTTACATAAGTAATCTAAATATTATTTTTTTTT271080.11137563120253142No Hit
GTTTGTTCCGCTTACTAATAAAGACAATTAAATGGCCGCGGTAACACTGA266890.10965413240240375No Hit
CGAGAAGTCCCCATGGAGCTTTACTGTAAGCTTATATATATAATTTTTTT266010.10929257656848673No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)