FastQCFastQC Report
Thu 20 Feb 2025
POC-219-TP4_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-219-TP4_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19455229
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1139930.5859247403358757No Hit
GTTGTAATAATTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAA401770.20651003388343567No Hit
GTTTTTTAAAAAGTAACGAAAATGAGCTATGACGCATGTTTAACTTTGAA271700.1396539716905928No Hit
CGATGGTTGAATCTTAATATGTTTGTTCCGCTTACTAATAAAGACAATTA234680.1206256682971966No Hit
GAAAAACATTTTTTTAAGGAACTCGGCAAAGTTAAACTTCGACTGTTTAC234300.1204303480570699No Hit
CAAAAACATAGCTTTTTGATTTTTATAAAAGGTGAAACCTGCCCGATGGT229290.11785520489118889No Hit
TTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTA226630.11648796321030196No Hit
GGATAACTAAGATAAACTAAAATTAGACGATTTTTTTCGAAATGGCGTTC216420.11124001675847661No Hit
GTTGAATCTTAATATGTTTGTTCCGCTTACTAATAAAGACAATTAAATGG206740.1062644906415648No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)