FastQCFastQC Report
Thu 20 Feb 2025
POC-219-TP4_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-219-TP4_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19455229
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGACTTCTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTTAA395940.20351341019938649No Hit
GGGGACTTCTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTTA340500.17501721516616434No Hit
CTCATTTTCGTTACTTTTTAAAAAACTGTCGCCCCAACAAAACTGTCTTA275080.14139129382645663No Hit
CGGGATACCATTCATACCGGTCAACAATTAATTGACAATTGATTACGCTA244610.12572969457208652No Hit
GTCGCAAACACTGTCGTTGATTAACTCTCAAACATTATTGCGCTGTTATC207240.10652149095752098No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)