FastQCFastQC Report
Thu 20 Feb 2025
POC-219-TP3_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-219-TP3_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21473974
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGACTTCTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTTAA4648512.1647180908387056No Hit
GGGGACTTCTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTTA3192301.4865902324367162No Hit
GGACTTCTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTTAAT1632900.7604088558549992No Hit
GCAGGTTTCACCTTTTATAAAAATCAAAAAGCTATGTTTTTGGTAAACAG1525600.7104413929159084No Hit
CTCATTTTCGTTACTTTTTAAAAAACTGTCGCCCCAACAAAACTGTCTTA1212850.5647999760081669No Hit
CTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATT1206730.5619500144686773No Hit
CGGGATACCATTCATACCGGTCAACAATTAATTGACAATTGATTACGCTA1136960.5294595215585155No Hit
TAACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATAT1067120.49693643104904567No Hit
TTCTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCA1033110.4810986545853133No Hit
CGGCCATTTAATTGTCTTTATTAGTAAGCGGAACAAACATATTAAGATTC962640.4482821856820726No Hit
ATTTAATTGTCTTTATTAGTAAGCGGAACAAACATATTAAGATTCAACCA956980.44564643693803485No Hit
CTAACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATA812420.3783277375673455No Hit
CTTCTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTC789180.36750533459712675No Hit
GTCATAGCTCATTTTCGTTACTTTTTAAAAAACTGTCGCCCCAACAAAAC741090.34511078387260785No Hit
GGCAGGTTTCACCTTTTATAAAAATCAAAAAGCTATGTTTTTGGTAAACA671000.3124712733656099No Hit
TGGGGACTTCTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTT644470.30011678322792046No Hit
GAAAAACTTTCGGGATACCATTCATACCGGTCAACAATTAATTGACAATT575070.26779859191410027No Hit
GTTTTTGGTAAACAGTCGAAGTTTAACTTTGCCGAGTTCCTTAAAAAAAT568660.264813583177478No Hit
TTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTTTTTT532770.2481003283323338No Hit
CGCTGTTATCCCCAGGGTAACTTTTATTTGTTTTCGTTATTTTTTTCATT519980.24214428125879264No Hit
CTTTATTAGTAAGCGGAACAAACATATTAAGATTCAACCATCGGGCAGGT491700.22897485113840596No Hit
CGAAAAAACAACATATTTTTGTTTTCTAATTGTAGATAGAAACCAAGCTG457790.2131836426736849No Hit
ATTTTCGTTACTTTTTAAAAAACTGTCGCCCCAACAAAACTGTCTTACTT450700.20988197154378596No Hit
CGCGGCCATTTAATTGTCTTTATTAGTAAGCGGAACAAACATATTAAGAT427820.19922721337000782No Hit
CGGGCAGGTTTCACCTTTTATAAAAATCAAAAAGCTATGTTTTTGGTAAA427490.19907353897327063No Hit
CTACATTATCACAGTCAGTGTTACCGCGGCCATTTAATTGTCTTTATTAG419050.19514319985671957No Hit
CACGCTTTTAACTCAAATCATGTATAACTTTAATGGATGAACAAACCAAC384410.17901204499921625No Hit
CCGGTCAACAATTAATTGACAATTGATTACGCTACATTATCACAGTCAGT383580.17862553060742276No Hit
TTTCGTACGAAAAAACAACATATTTTTGTTTTCTAATTGTAGATAGAAAC378130.17608757466130862No Hit
CGTCATAGCTCATTTTCGTTACTTTTTAAAAAACTGTCGCCCCAACAAAA374960.1746113690926514No Hit
CCCAGGGTAACTTTTATTTGTTTTCGTTATTTTTTTCATTCAAAATAACG361480.16833400282593244No Hit
GTCAGTGTTACCGCGGCCATTTAATTGTCTTTATTAGTAAGCGGAACAAA357620.1665364780640975No Hit
CCCCAGGGTAACTTTTATTTGTTTTCGTTATTTTTTTCATTCAAAATAAC356710.16611270927309496No Hit
CGCAAACACTGTCGTTGATTAACTCTCAAACATTATTGCGCTGTTATCCC355710.16564702928298228No Hit
GACTTCTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTTAATT353420.16458062210562424No Hit
GTCGCAAACACTGTCGTTGATTAACTCTCAAACATTATTGCGCTGTTATC343100.15977480460766133No Hit
CTTTTATTTGTTTTCGTTATTTTTTTCATTCAAAATAACGGATCAAAAAA339050.157888800647705No Hit
GCTGGTTCCAAGCCTACATTTTTATTGTCTAAATTTAATTTTTTTTTAAA332190.15469423591553197No Hit
TTTATTAGTAAGCGGAACAAACATATTAAGATTCAACCATCGGGCAGGTT329340.15336704794371084No Hit
CACGCTTTTTGCTTTTTATTAAAAAATAAATCCTTAAGTCTTACCGCGTC326990.15227269996694603No Hit
ATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTTTTT316460.1473690896710595No Hit
CCACAATCACCAGTTTTTGCTCACACAATTCATGATTAAATCACTTGGTT311730.1451664233178265No Hit
GTCAACAATTAATTGACAATTGATTACGCTACATTATCACAGTCAGTGTT309090.14393702814392903No Hit
GGGGCTTCTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTTAA307450.1431733129601442No Hit
GCGGAACAAACATATTAAGATTCAACCATCGGGCAGGTTTCACCTTTTAT303400.14128730900018785No Hit
CTCATCAATAAAAAAATTTCAAAGTTAAACATGCGTCATAGCTCATTTTC299470.13945718663904502No Hit
CTGATTTGAGCTTTCTCTTTTAACAAAAAATCTTTTCATTTATTGTTTGT299460.13945252983914389No Hit
TCTGATTTGAGCTTTCTCTTTTAACAAAAAATCTTTTCATTTATTGTTTG299430.13943855943944053No Hit
CAGGTTTCACCTTTTATAAAAATCAAAAAGCTATGTTTTTGGTAAACAGT295590.13765034827740782No Hit
CTAAAATTAGACGATTTTTTTCGAAATGGCGTTCAGTTGCTTATTGGTTG290720.13538248672555905No Hit
CTTTAATGGATGAACAAACCAACCCTTACGAGTGACTACACCGTAGGACG288010.13412049395235368No Hit
CAAACATATTAAGATTCAACCATCGGGCAGGTTTCACCTTTTATAAAAAT284910.13267688598300437No Hit
CATAGCTCATTTTCGTTACTTTTTAAAAAACTGTCGCCCCAACAAAACTG284720.13258840678488296No Hit
ATTTTTTTCATTCAAAATAACGGATCAAAAAACACACCAAAAATGTCTCA283200.13188057319991167No Hit
ATCACAGTCAGTGTTACCGCGGCCATTTAATTGTCTTTATTAGTAAGCGG283100.13183400520090038No Hit
GCCACAATCACCAGTTTTTGCTCACACAATTCATGATTAAATCACTTGGT276410.12871860606704655No Hit
GAACAAACATATTAAGATTCAACCATCGGGCAGGTTTCACCTTTTATAAA273980.12758700369107273No Hit
CGGGGACTTCTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTT273050.12715392130026795No Hit
CGCTACATTATCACAGTCAGTGTTACCGCGGCCATTTAATTGTCTTTATT272980.12712132370096005No Hit
GTAACTTTTATTTGTTTTCGTTATTTTTTTCATTCAAAATAACGGATCAA271860.12659976211203386No Hit
CTGGTTCCAAGCCTACATTTTTATTGTCTAAATTTAATTTTTTTTTAAAC266240.12398264056760057No Hit
GCTCATTTTCGTTACTTTTTAAAAAACTGTCGCCCCAACAAAACTGTCTT265620.12369391897373071No Hit
GGATACCATTCATACCGGTCAACAATTAATTGACAATTGATTACGCTACA264640.12323755258342027No Hit
CTGTTATCCCCAGGGTAACTTTTATTTGTTTTCGTTATTTTTTTCATTCA263840.12286500859133014No Hit
CGCTTTTTGCTTTTTATTAAAAAATAAATCCTTAAGTCTTACCGCGTCGG262320.12215717500635885No Hit
GAACAAACCAACCCTTACGAGTGACTACACCGTAGGACGTTACAATTCAA262020.12201747100932506No Hit
AAAAACTTTCGGGATACCATTCATACCGGTCAACAATTAATTGACAATTG256620.11950279906271657No Hit
GGAACAAACATATTAAGATTCAACCATCGGGCAGGTTTCACCTTTTATAA255010.11875305427863514No Hit
TTTAATTGTCTTTATTAGTAAGCGGAACAAACATATTAAGATTCAACCAT252480.11757488390365005No Hit
CTAATCACAAATTTTCTGAGATTTTTGCCACAATCACCAGTTTTTGCTCA252150.11742120950691287No Hit
CTCTCTTTCAACTTTCCTTTACAGTACTTTCATTATCAGTATAAACTAAT248710.11581927034092525No Hit
GTTCGATTAGCCTTTCACCCCTAATCACAAATTTTCTGAGATTTTTGCCA247660.11533030635130695No Hit
CTTCACTACAGATTTAATTTCATTGAATATTTTTTTAAGACAGAAAAACT245890.11450605276880749No Hit
AGCGGAACAAACATATTAAGATTCAACCATCGGGCAGGTTTCACCTTTTA244450.11383547358304522No Hit
GCTATTTCCAAGTTCGATTAGCCTTTCACCCCTAATCACAAATTTTCTGA241080.11226613201636548No Hit
CGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGA241030.11224284801685984No Hit
CACAAGCACAACTCCAATTTCACTCTCCACTACTCTCGGAATCTCGTTTG240770.11212177121943055No Hit
GGAAATTTGACTAAAAAAAAATTACTTAAAATTTTCCATTTTAAATATGC238260.11095291444424772No Hit
GTTACCGCGGCCATTTAATTGTCTTTATTAGTAAGCGGAACAAACATATT233760.10885735448874065No Hit
CACAACTCCAATTTCACTCTCCACTACTCTCGGAATCTCGTTTGATTTTT230010.1071110545258181No Hit
GTCGAAGTTTAACTTTGCCGAGTTCCTTAAAAAAATGTTTTTCTTTTTTT228240.10628680094331865No Hit
GTAGCATCTTCACTACAGATTTAATTTCATTGAATATTTTTTTAAGACAG226230.10535078416319214No Hit
AAACATATTAAGATTCAACCATCGGGCAGGTTTCACCTTTTATAAAAATC221620.10320399940877269No Hit
GTTATCCCCAGGGTAACTTTTATTTGTTTTCGTTATTTTTTTCATTCAAA221270.10304101141223325No Hit
TTCTGATTTGAGCTTTCTCTTTTAACAAAAAATCTTTTCATTTATTGTTT221210.10301307061282648No Hit
AAACAGTCGAAGTTTAACTTTGCCGAGTTCCTTAAAAAAATGTTTTTCTT217580.10132265224871745No Hit
TTCGGGATACCATTCATACCGGTCAACAATTAATTGACAATTGATTACGC215570.10038663546859095No Hit
ATAGAAACCAAGCTGTGTCACCACGCTTTTAACTCAAATCATGTATAACT215160.10019570667264477No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)