Bismark report for: ../../data/03-Peve-bismark/POR-83-TP3_R1_001.fastp-trim.fq.gz and ../../data/03-Peve-bismark/POR-83-TP3_R2_001.fastp-trim.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/srlab/sr320/github/timeseries_molecular/E-Peve/data/ with the specified options: -q --score-min L,0,-0.6 -p 12 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 29875318 Number of paired-end alignments with a unique best hit: 19369386 Mapping efficiency: 64.8% Sequence pairs with no alignments under any condition: 8051274 Sequence pairs did not map uniquely: 2454658 Sequence pairs which were discarded because genomic sequence could not be extracted: 3500 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 9699836 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 9666050 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 985072911 Total methylated C's in CpG context: 13222740 Total methylated C's in CHG context: 801199 Total methylated C's in CHH context: 3177494 Total methylated C's in Unknown context: 18999 Total unmethylated C's in CpG context: 142622877 Total unmethylated C's in CHG context: 171947878 Total unmethylated C's in CHH context: 653300723 Total unmethylated C's in Unknown context: 1251502 C methylated in CpG context: 8.5% C methylated in CHG context: 0.5% C methylated in CHH context: 0.5% C methylated in Unknown context (CN or CHN): 1.5% Bismark completed in 0d 2h 14m 14s