Bismark report for: ../../data/03-Peve-bismark/POR-72-TP3_R1_001.fastp-trim.fq.gz and ../../data/03-Peve-bismark/POR-72-TP3_R2_001.fastp-trim.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/srlab/sr320/github/timeseries_molecular/E-Peve/data/ with the specified options: -q --score-min L,0,-0.6 -p 12 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 33295631 Number of paired-end alignments with a unique best hit: 20665456 Mapping efficiency: 62.1% Sequence pairs with no alignments under any condition: 9795552 Sequence pairs did not map uniquely: 2834623 Sequence pairs which were discarded because genomic sequence could not be extracted: 3452 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 10349466 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 10312538 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 1045257328 Total methylated C's in CpG context: 13813436 Total methylated C's in CHG context: 832938 Total methylated C's in CHH context: 3306958 Total methylated C's in Unknown context: 20824 Total unmethylated C's in CpG context: 151216420 Total unmethylated C's in CHG context: 182623527 Total unmethylated C's in CHH context: 693464049 Total unmethylated C's in Unknown context: 1333771 C methylated in CpG context: 8.4% C methylated in CHG context: 0.5% C methylated in CHH context: 0.5% C methylated in Unknown context (CN or CHN): 1.5% Bismark completed in 0d 2h 27m 11s